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The map label for this gene is otsA [H]

Identifier: 157368828

GI number: 157368828

Start: 648308

End: 648955

Strand: Reverse

Name: otsA [H]

Synonym: Spro_0583

Alternate gene names: 157368828

Gene position: 648955-648308 (Counterclockwise)

Preceding gene: 157368829

Following gene: 157368827

Centisome position: 11.91

GC content: 57.87

Gene sequence:

>648_bases
ATGTCTCGTTTGGTTTTGATCTCGAATAAACAGTGTGACAGCAAAGACCTGGCCTGCGCCACGCAGGCGCTATCCTCCGT
TGACGCCAGCGATGATGAGCAAGGCCTGTGGCTGGGCTGGAACGGCGACGTGCAGAACTTTGGCGCCGCGCCGCTGCGCC
CGGTCAGCCATCAGCACCGCAGCGGCTATCAGCAAGTGACCTTTCCGCTGTCGACCGAAGAGTTTCACTTCCACTATCAG
GGTTACTATCACGATGGCCTGTGGCCGGTGTTCCATAACCACCCGGAGAAGGCGCATTTCACGGCCGAGAACTACCGCGC
TTACCGCCAGCTCAACCAGCACTTCGCCGATATCGCCTGCGAGTATGTGTGCCCGAACGACATCATCTGCATTGATGATT
ATCAGCTGCTGCCCTGCGGCCAGGCGCTGAAGGAACAGGGGCTGTTGAACCCCTGCGCGTTCTTCTTCCATTTGCCGTTC
CCGTCGGCGGCGCTGCTCAGGCAGATCCCCGAGCACCAGGGGTTAATCGAGTCGCTATTTTTCTACGACCTGATTGGCTT
TCAGTCGCTGGACGATCGCAACAACTTCCTCAATTATCTGGCCAATGAACCCGGTCGAGATGCTGCCCGACGATCGACTG
CAGGTTAA

Upstream 100 bases:

>100_bases
GCAGGTCACCGCCGCGGAAGTCAGTTGGATGCTTTAACCCGCCGGGTTCTGCATTCATCGGGCGAATATATTCGCCCCAT
AATTGAACAAGGAACAAAAC

Downstream 100 bases:

>100_bases
CGGCCATATCTTTGCCACCGGCATCTTCCCGGCCGGGATCAACGCCCGCAACATCTACTGACCCACCGCCCGGTTGGCGT
TGCCGCACAGCGCCCAACCG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSRLVLISNKQCDSKDLACATQALSSVDASDDEQGLWLGWNGDVQNFGAAPLRPVSHQHRSGYQQVTFPLSTEEFHFHYQ
GYYHDGLWPVFHNHPEKAHFTAENYRAYRQLNQHFADIACEYVCPNDIICIDDYQLLPCGQALKEQGLLNPCAFFFHLPF
PSAALLRQIPEHQGLIESLFFYDLIGFQSLDDRNNFLNYLANEPGRDAARRSTAG

Sequences:

>Translated_215_residues
MSRLVLISNKQCDSKDLACATQALSSVDASDDEQGLWLGWNGDVQNFGAAPLRPVSHQHRSGYQQVTFPLSTEEFHFHYQ
GYYHDGLWPVFHNHPEKAHFTAENYRAYRQLNQHFADIACEYVCPNDIICIDDYQLLPCGQALKEQGLLNPCAFFFHLPF
PSAALLRQIPEHQGLIESLFFYDLIGFQSLDDRNNFLNYLANEPGRDAARRSTAG
>Mature_214_residues
SRLVLISNKQCDSKDLACATQALSSVDASDDEQGLWLGWNGDVQNFGAAPLRPVSHQHRSGYQQVTFPLSTEEFHFHYQG
YYHDGLWPVFHNHPEKAHFTAENYRAYRQLNQHFADIACEYVCPNDIICIDDYQLLPCGQALKEQGLLNPCAFFFHLPFP
SAALLRQIPEHQGLIESLFFYDLIGFQSLDDRNNFLNYLANEPGRDAARRSTAG

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=204, Percent_Identity=32.3529411764706, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI115533558, Length=157, Percent_Identity=26.1146496815287, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115533560, Length=157, Percent_Identity=26.1146496815287, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319602, Length=141, Percent_Identity=32.6241134751773, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323917, Length=137, Percent_Identity=28.4671532846715, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323537, Length=137, Percent_Identity=29.1970802919708, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920676, Length=185, Percent_Identity=28.6486486486486, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 24499; Mature: 24368

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVLISNKQCDSKDLACATQALSSVDASDDEQGLWLGWNGDVQNFGAAPLRPVSHQHR
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
SGYQQVTFPLSTEEFHFHYQGYYHDGLWPVFHNHPEKAHFTAENYRAYRQLNQHFADIAC
CCCEEEEEECCCCEEEEEEEEEEECCCCHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHE
EYVCPNDIICIDDYQLLPCGQALKEQGLLNPCAFFFHLPFPSAALLRQIPEHQGLIESLF
HEECCCCEEEECCCEECCCCHHHHHCCCCCHHHHEEECCCCHHHHHHHCCHHHHHHHHHH
FYDLIGFQSLDDRNNFLNYLANEPGRDAARRSTAG
HHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SRLVLISNKQCDSKDLACATQALSSVDASDDEQGLWLGWNGDVQNFGAAPLRPVSHQHR
CCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
SGYQQVTFPLSTEEFHFHYQGYYHDGLWPVFHNHPEKAHFTAENYRAYRQLNQHFADIAC
CCCEEEEEECCCCEEEEEEEEEEECCCCHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHE
EYVCPNDIICIDDYQLLPCGQALKEQGLLNPCAFFFHLPFPSAALLRQIPEHQGLIESLF
HEECCCCEEEECCCEECCCCHHHHHCCCCCHHHHEEECCCCHHHHHHHCCHHHHHHHHHH
FYDLIGFQSLDDRNNFLNYLANEPGRDAARRSTAG
HHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA