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The map label for this gene is ispB [H]

Identifier: 157368719

GI number: 157368719

Start: 522892

End: 523863

Strand: Reverse

Name: ispB [H]

Synonym: Spro_0472

Alternate gene names: 157368719

Gene position: 523863-522892 (Counterclockwise)

Preceding gene: 157368724

Following gene: 157368717

Centisome position: 9.61

GC content: 54.42

Gene sequence:

>972_bases
ATGAACCTAGAGCAAATTACCGAGTTAACCGCGCAAGATATGGCGGCCGTGAACGCAACAATTCTCGAACAGCTGAATTC
CGATGTCACGCTCATCAATCAGCTTGGCTATTACATTATCAGTGGTGGCGGTAAACGCATCCGCCCGATGATCGCCGTTC
TGGCGGCGCGGGCTTTGCATTATCAGGGTGATAAACACATTACCGTTGCCGCGCTGATCGAATTCATTCACACCGCAACT
CTACTGCATGATGACGTGGTGGATGAATCAGATATGCGCCGCGGCAAGGCCACCGCCAATGCCGCCTTCGGCAATGCCGC
CAGCGTCCTGGTCGGCGACTTCATCTATACGCGTGCTTTCCAGATGATGACCAGTCTGGAATCGCTGCGCGTGCTGGCAC
TGATGTCTGAGGCGGTCAACGTGATTGCCGAAGGTGAAGTTCTGCAGTTAATGAACGTCAACGATCCTGACATTACCGAA
GAAAGCTACATGCGGGTGATCTACAGCAAAACCGCACGTCTGTTTGAAGCGGCGGCGCAATCTTCAGCAATCCTGTCAGA
CGCTAGCGTTGCAGAAGAAAAAGCGCTGCAGGATTACGGTCGCTATCTCGGCACCGCTTTCCAGTTGATCGACGATCTGC
TCGATTACAGTGCCGACGGCAGTACACTGGGTAAAAATACCGGTGACGATCTCAATGAAGGGAAGCCGACATTGCCGCTA
CTGCACGCCATGCATAACAGCGAAGGCGAGCAGAAAGCCATGATCCGCGGGGCGATTGAACAGGGTAACGGCCGTCATTT
GCTGGAACCGGTGTTGCAAGCCATGCAGCAATGCGGCTCACTGGAGTATACCCGCCAACGGGCTGAGGAAGAGGCTGACA
AGGCCATTGCCGCCTTGCAGGTTCTACCCGAATCGCCACATCGTTCCGCGCTGGAAGGATTAGCGCACCTTGCGGTCCAA
CGCGATTTTTAA

Upstream 100 bases:

>100_bases
GGCCGTTTTTCAAGTACAATCAGAGGGACAGTTACCGCCATGTACCGATGTGAGCAGCGATCTCAACGCGGTGAAGAGAA
ACACAGCTGAAAAAAATACA

Downstream 100 bases:

>100_bases
GAAACTATAAGAATATTATTGTTTCCACAGACCCGCACTGCGGGTCTGATCATTTATCTGCTGAAATACGTGCCAGCAAG
CTACCGATCCCTTCTCGTAT

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLEQITELTAQDMAAVNATILEQLNSDVTLINQLGYYIISGGGKRIRPMIAVLAARALHYQGDKHITVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLESLRVLALMSEAVNVIAEGEVLQLMNVNDPDITE
ESYMRVIYSKTARLFEAAAQSSAILSDASVAEEKALQDYGRYLGTAFQLIDDLLDYSADGSTLGKNTGDDLNEGKPTLPL
LHAMHNSEGEQKAMIRGAIEQGNGRHLLEPVLQAMQQCGSLEYTRQRAEEEADKAIAALQVLPESPHRSALEGLAHLAVQ
RDF

Sequences:

>Translated_323_residues
MNLEQITELTAQDMAAVNATILEQLNSDVTLINQLGYYIISGGGKRIRPMIAVLAARALHYQGDKHITVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLESLRVLALMSEAVNVIAEGEVLQLMNVNDPDITE
ESYMRVIYSKTARLFEAAAQSSAILSDASVAEEKALQDYGRYLGTAFQLIDDLLDYSADGSTLGKNTGDDLNEGKPTLPL
LHAMHNSEGEQKAMIRGAIEQGNGRHLLEPVLQAMQQCGSLEYTRQRAEEEADKAIAALQVLPESPHRSALEGLAHLAVQ
RDF
>Mature_323_residues
MNLEQITELTAQDMAAVNATILEQLNSDVTLINQLGYYIISGGGKRIRPMIAVLAARALHYQGDKHITVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLESLRVLALMSEAVNVIAEGEVLQLMNVNDPDITE
ESYMRVIYSKTARLFEAAAQSSAILSDASVAEEKALQDYGRYLGTAFQLIDDLLDYSADGSTLGKNTGDDLNEGKPTLPL
LHAMHNSEGEQKAMIRGAIEQGNGRHLLEPVLQAMQQCGSLEYTRQRAEEEADKAIAALQVLPESPHRSALEGLAHLAVQ
RDF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=324, Percent_Identity=31.4814814814815, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4758430, Length=286, Percent_Identity=25.5244755244755, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI83700220, Length=286, Percent_Identity=25.5244755244755, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=85.0931677018634, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1786623, Length=243, Percent_Identity=28.8065843621399, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=311, Percent_Identity=31.1897106109325, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=302, Percent_Identity=35.0993377483444, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325188, Length=242, Percent_Identity=26.4462809917355, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24651612, Length=300, Percent_Identity=33, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI281365769, Length=278, Percent_Identity=23.3812949640288, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24660002, Length=278, Percent_Identity=23.3812949640288, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35219; Mature: 35219

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEQITELTAQDMAAVNATILEQLNSDVTLINQLGYYIISGGGKRIRPMIAVLAARALH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHH
YQGDKHITVAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLESLRVLALMSEAVNVIAEGEVLQLMNVNDPDITEESYMRVIYSKTARLFEAAAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSAILSDASVAEEKALQDYGRYLGTAFQLIDDLLDYSADGSTLGKNTGDDLNEGKPTLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LHAMHNSEGEQKAMIRGAIEQGNGRHLLEPVLQAMQQCGSLEYTRQRAEEEADKAIAALQ
HHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VLPESPHRSALEGLAHLAVQRDF
HCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLEQITELTAQDMAAVNATILEQLNSDVTLINQLGYYIISGGGKRIRPMIAVLAARALH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHH
YQGDKHITVAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLESLRVLALMSEAVNVIAEGEVLQLMNVNDPDITEESYMRVIYSKTARLFEAAAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSAILSDASVAEEKALQDYGRYLGTAFQLIDDLLDYSADGSTLGKNTGDDLNEGKPTLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LHAMHNSEGEQKAMIRGAIEQGNGRHLLEPVLQAMQQCGSLEYTRQRAEEEADKAIAALQ
HHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VLPESPHRSALEGLAHLAVQRDF
HCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]