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The map label for this gene is mpl [H]

Identifier: 157368712

GI number: 157368712

Start: 517301

End: 518680

Strand: Direct

Name: mpl [H]

Synonym: Spro_0465

Alternate gene names: 157368712

Gene position: 517301-518680 (Clockwise)

Preceding gene: 157368710

Following gene: 157368716

Centisome position: 9.49

GC content: 58.19

Gene sequence:

>1380_bases
ATGCGCATTCACATTCTTGGGATCTGTGGCACCTTTATGGGCGGGCTGGCGGTGCTGGCTCGTTCGCTAGGGCATGAGGT
GACCGGCTCTGACGCTAATGTCTATCCGCCGATGAGCACGCTGTTGGAGAAAGAAGGGATCGCACTGATCCAGGGCTACG
ATCCGGCTCAGTTGGATCCTGCACCGGATCTGGTGATCATCGGTAACGCCATGACGCGCGGTAATCCGTGTGTTGAAGCA
GTACTGGAGCAGGGCATCCCTTACGTTTCCGGCCCGCAGTGGCTGCATGATTATGTGCTGCGCGATCGCTGGGTACTGGC
CGTTGCCGGGACACACGGCAAGACCACCACCGCCGGCATGGCAACCTGGATCCTGGAAGCCTGCGGCTACCAGCCGGGCT
TTGTTATCGGTGGCGTGCCGGGCAACTTTGACGTTTCTGCTCGCCTGGGGGGCAGCCCGTTCTTTGTCATCGAAGCCGAT
GAATATGACTGCGCGTTCTTCGACAAGCGTTCCAAGTTTGTGCATTACAGCCCGCGTACGCTGATCCTGAACAATCTGGA
GTTTGATCACGCCGATATCTTCGACGATCTGAAAGCGATCCAGAAACAGTTCCACCATCTGGTGCGTTTGGTGCCAGGCA
AGGGCAAAATTATTTTGCCTGATAACGACAGCCACCTGAAACAGGTGATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTG
GTTGGGGAAGAAGGCGCATGGCGTGCACAGAAACTGACGCCGGACGCCAGCCATTATGCGGTTTTCCTTAATGGTGAGCA
GGTGGGCGAAGTTAAGTGGTCGCTGGTCGGTGAACACAACATGCATAACGGTTTGATGGCCATTGCCGCCACGCGCCATG
TGGGTGTGTTGCCTGAAGATGCCTGCCGCGCGCTCGGCGATTTTATCAATGCCCGCCGTCGTCTGGAGCTGCGTGGTGAA
GCCAATGGCGTCACGGTATATGACGACTTTGCCCATCATCCAACGGCAATTCTGGCGACCCTGGCTGCGTTGCGTAGCAA
GGTGGGTGGCACTGCCCGCATTCTGGCGGTGCTTGAGCCGCGCTCCAACACCATGAAAATGGGCATCAGCAAGAACGATC
TGGCCCCGTCGCTGGGTCGTGCCGATGAAGTCTTCCTGTTCCAGCCGCACCATATCCCCTGGCAGGTGGCGGAAGTGGCG
GAAGCCTGCGTACAGCCGGCCCACTGGAGCGCCGATCTGGATACGCTGGTGGATATGATCGTGAAAACGGCTCAGCCGGG
CGACCATATTCTGGTGATGAGTAACGGCGGCTTTGGGGGTATTCACGATCGGCTGCTGGAGGCTTTGGCCAACAAACCCG
ATCCGAAAGCCACCTATTGA

Upstream 100 bases:

>100_bases
GTAGCCCAAAGATGAACGCTATTCATAGGCAAATCCATTTCTTCTGGACGATTCTGAGCGTTAGAATGTGCCAACATTCG
ACGTACTCTATGAGAAAACT

Downstream 100 bases:

>100_bases
TTTTGATGACAAAGTCGTCTTTGAACCCGAGATACTCGGGCCTAGCGCACCGAGGGGCCATTATGGGACACATCCATGTG
TCCCACCCTGCGGGCCGACT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRIHILGICGTFMGGLAVLARSLGHEVTGSDANVYPPMSTLLEKEGIALIQGYDPAQLDPAPDLVIIGNAMTRGNPCVEA
VLEQGIPYVSGPQWLHDYVLRDRWVLAVAGTHGKTTTAGMATWILEACGYQPGFVIGGVPGNFDVSARLGGSPFFVIEAD
EYDCAFFDKRSKFVHYSPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRLVPGKGKIILPDNDSHLKQVMAMGCWSEQEL
VGEEGAWRAQKLTPDASHYAVFLNGEQVGEVKWSLVGEHNMHNGLMAIAATRHVGVLPEDACRALGDFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEPRSNTMKMGISKNDLAPSLGRADEVFLFQPHHIPWQVAEVA
EACVQPAHWSADLDTLVDMIVKTAQPGDHILVMSNGGFGGIHDRLLEALANKPDPKATY

Sequences:

>Translated_459_residues
MRIHILGICGTFMGGLAVLARSLGHEVTGSDANVYPPMSTLLEKEGIALIQGYDPAQLDPAPDLVIIGNAMTRGNPCVEA
VLEQGIPYVSGPQWLHDYVLRDRWVLAVAGTHGKTTTAGMATWILEACGYQPGFVIGGVPGNFDVSARLGGSPFFVIEAD
EYDCAFFDKRSKFVHYSPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRLVPGKGKIILPDNDSHLKQVMAMGCWSEQEL
VGEEGAWRAQKLTPDASHYAVFLNGEQVGEVKWSLVGEHNMHNGLMAIAATRHVGVLPEDACRALGDFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEPRSNTMKMGISKNDLAPSLGRADEVFLFQPHHIPWQVAEVA
EACVQPAHWSADLDTLVDMIVKTAQPGDHILVMSNGGFGGIHDRLLEALANKPDPKATY
>Mature_459_residues
MRIHILGICGTFMGGLAVLARSLGHEVTGSDANVYPPMSTLLEKEGIALIQGYDPAQLDPAPDLVIIGNAMTRGNPCVEA
VLEQGIPYVSGPQWLHDYVLRDRWVLAVAGTHGKTTTAGMATWILEACGYQPGFVIGGVPGNFDVSARLGGSPFFVIEAD
EYDCAFFDKRSKFVHYSPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRLVPGKGKIILPDNDSHLKQVMAMGCWSEQEL
VGEEGAWRAQKLTPDASHYAVFLNGEQVGEVKWSLVGEHNMHNGLMAIAATRHVGVLPEDACRALGDFINARRRLELRGE
ANGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEPRSNTMKMGISKNDLAPSLGRADEVFLFQPHHIPWQVAEVA
EACVQPAHWSADLDTLVDMIVKTAQPGDHILVMSNGGFGGIHDRLLEALANKPDPKATY

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=454, Percent_Identity=86.5638766519824, Blast_Score=823, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=286, Percent_Identity=30.0699300699301, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49998; Mature: 49998

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHILGICGTFMGGLAVLARSLGHEVTGSDANVYPPMSTLLEKEGIALIQGYDPAQLDP
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCC
APDLVIIGNAMTRGNPCVEAVLEQGIPYVSGPQWLHDYVLRDRWVLAVAGTHGKTTTAGM
CCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILEACGYQPGFVIGGVPGNFDVSARLGGSPFFVIEADEYDCAFFDKRSKFVHYSPRT
HHHHHHHHCCCCCEEECCCCCCCEEEEEECCCEEEEEECCCCCEEEECCCCCEEEECCCE
LILNNLEFDHADIFDDLKAIQKQFHHLVRLVPGKGKIILPDNDSHLKQVMAMGCWSEQEL
EEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHHHCCCCHHHH
VGEEGAWRAQKLTPDASHYAVFLNGEQVGEVKWSLVGEHNMHNGLMAIAATRHVGVLPED
CCCCCCCCHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHH
ACRALGDFINARRRLELRGEANGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEP
HHHHHHHHHHHHHEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
RSNTMKMGISKNDLAPSLGRADEVFLFQPHHIPWQVAEVAEACVQPAHWSADLDTLVDMI
CCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHDRLLEALANKPDPKATY
HHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRIHILGICGTFMGGLAVLARSLGHEVTGSDANVYPPMSTLLEKEGIALIQGYDPAQLDP
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCC
APDLVIIGNAMTRGNPCVEAVLEQGIPYVSGPQWLHDYVLRDRWVLAVAGTHGKTTTAGM
CCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHH
ATWILEACGYQPGFVIGGVPGNFDVSARLGGSPFFVIEADEYDCAFFDKRSKFVHYSPRT
HHHHHHHHCCCCCEEECCCCCCCEEEEEECCCEEEEEECCCCCEEEECCCCCEEEECCCE
LILNNLEFDHADIFDDLKAIQKQFHHLVRLVPGKGKIILPDNDSHLKQVMAMGCWSEQEL
EEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHHHCCCCHHHH
VGEEGAWRAQKLTPDASHYAVFLNGEQVGEVKWSLVGEHNMHNGLMAIAATRHVGVLPED
CCCCCCCCHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHH
ACRALGDFINARRRLELRGEANGVTVYDDFAHHPTAILATLAALRSKVGGTARILAVLEP
HHHHHHHHHHHHHEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECC
RSNTMKMGISKNDLAPSLGRADEVFLFQPHHIPWQVAEVAEACVQPAHWSADLDTLVDMI
CCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VKTAQPGDHILVMSNGGFGGIHDRLLEALANKPDPKATY
HHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]