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The map label for this gene is rlmB [H]

Identifier: 157368686

GI number: 157368686

Start: 492117

End: 492848

Strand: Direct

Name: rlmB [H]

Synonym: Spro_0439

Alternate gene names: 157368686

Gene position: 492117-492848 (Clockwise)

Preceding gene: 157368685

Following gene: 157368687

Centisome position: 9.03

GC content: 59.7

Gene sequence:

>732_bases
ATGAGCGAAATTATTTACGGTATTCATGCCGTCAAGGCCCTGTTAGATAACGATCCGCAACGCTTCCTGGAAGTGTTTAT
CCTCAAAGGGCGCGACGATCGCCGCTTGCAGCCGCTGATCGCCGAGCTGGAAGCGACCGGTATTGTTATCCAGGTGGCTA
ACCGCCAGTGGCTGGATGAGAAGGTTGAGGGCGCGGTGCATCAGGGGATTATCGCCCGGGTGCGCGAAGGGCGTCAGTAT
CAGGAAAACGATTTGCCGGCGCTGTTGGAAAGCGTGGACACGCCTTTCCTGCTGGTGTTGGACGGCGTGACCGACCCGCA
CAACCTCGGCGCCTGTCTTCGCAGTGCCGATGCGGCCGGTGTACACGCGGTGATCGTCCCGCGCGATCGCTCTGCCCAGT
TGAATGCTACCGCCAAGAAAGTGGCCTGCGGCGCGGCGGAAAACGTTCCGTTGATTCGCGTCACCAACCTGGCGCGTACC
CTGCGTTTGCTGCAGGAAATGAACGTCTGGGTCGTCGGCACTGCCGGCGAAGCCGATCACACTTTGTTCCAGAGCAAAAT
GACTGGCCCGATGGCGCTGGTGATGGGGGCAGAGGGCGAAGGTATGCGTCGTCTGACTCGCGAGCACTGTGACGAGTTGA
TCAGCATCCCAATGGCCGGGACGGTGTCTTCGCTGAACGTATCAGTCGCCACCGGTATCTGTCTGTTTGAAGCGGTGCGT
CAACGCAGCTGA

Upstream 100 bases:

>100_bases
CCAAACGCGCCGGCAAGAAGAAAGGTGCTGAGCAGGCTTAAGCTGCCACACGTCATTATCACCGGCTGGCATACGCCAGC
CGCACAGTTAAAAGAGCATC

Downstream 100 bases:

>100_bases
TTTACCGTTTAGGGCGCAGAATTTTGCGCCCTTGTTGCTTTCTCAATTCCCACCCTGTGATCTCACCTGCGGCAGAAATC
CCCCTGACGACCATACTTAC

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSEIIYGIHAVKALLDNDPQRFLEVFILKGRDDRRLQPLIAELEATGIVIQVANRQWLDEKVEGAVHQGIIARVREGRQY
QENDLPALLESVDTPFLLVLDGVTDPHNLGACLRSADAAGVHAVIVPRDRSAQLNATAKKVACGAAENVPLIRVTNLART
LRLLQEMNVWVVGTAGEADHTLFQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGTVSSLNVSVATGICLFEAVR
QRS

Sequences:

>Translated_243_residues
MSEIIYGIHAVKALLDNDPQRFLEVFILKGRDDRRLQPLIAELEATGIVIQVANRQWLDEKVEGAVHQGIIARVREGRQY
QENDLPALLESVDTPFLLVLDGVTDPHNLGACLRSADAAGVHAVIVPRDRSAQLNATAKKVACGAAENVPLIRVTNLART
LRLLQEMNVWVVGTAGEADHTLFQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGTVSSLNVSVATGICLFEAVR
QRS
>Mature_242_residues
SEIIYGIHAVKALLDNDPQRFLEVFILKGRDDRRLQPLIAELEATGIVIQVANRQWLDEKVEGAVHQGIIARVREGRQYQ
ENDLPALLESVDTPFLLVLDGVTDPHNLGACLRSADAAGVHAVIVPRDRSAQLNATAKKVACGAAENVPLIRVTNLARTL
RLLQEMNVWVVGTAGEADHTLFQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGTVSSLNVSVATGICLFEAVRQ
RS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=222, Percent_Identity=32.4324324324324, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=84.3621399176955, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1788935, Length=240, Percent_Identity=28.75, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790083, Length=150, Percent_Identity=32, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=253, Percent_Identity=28.4584980237154, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=31.5151515151515, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26507; Mature: 26376

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIIYGIHAVKALLDNDPQRFLEVFILKGRDDRRLQPLIAELEATGIVIQVANRQWLDE
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHH
KVEGAVHQGIIARVREGRQYQENDLPALLESVDTPFLLVLDGVTDPHNLGACLRSADAAG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCC
VHAVIVPRDRSAQLNATAKKVACGAAENVPLIRVTNLARTLRLLQEMNVWVVGTAGEADH
EEEEEEECCCCCCCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEEECCCCHH
TLFQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGTVSSLNVSVATGICLFEAVR
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHH
QRS
HCC
>Mature Secondary Structure 
SEIIYGIHAVKALLDNDPQRFLEVFILKGRDDRRLQPLIAELEATGIVIQVANRQWLDE
CHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCHHHHH
KVEGAVHQGIIARVREGRQYQENDLPALLESVDTPFLLVLDGVTDPHNLGACLRSADAAG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCC
VHAVIVPRDRSAQLNATAKKVACGAAENVPLIRVTNLARTLRLLQEMNVWVVGTAGEADH
EEEEEEECCCCCCCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEEECCCCHH
TLFQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGTVSSLNVSVATGICLFEAVR
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHH
QRS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]