Click here to switch to the map view.

The map label for this gene is amiB [H]

Identifier: 157368675

GI number: 157368675

Start: 478431

End: 480224

Strand: Direct

Name: amiB [H]

Synonym: Spro_0428

Alternate gene names: 157368675

Gene position: 478431-480224 (Clockwise)

Preceding gene: 157368674

Following gene: 157368676

Centisome position: 8.78

GC content: 58.81

Gene sequence:

>1794_bases
ATGACGTATGCGTTGAGAAAACTATTCGTAATCGCCCTGATCGCCGTATTGGGGCCTTTGGGTGCGGCCAGTGCGCTGGC
CGCATCGTCGCTGTCTGATATTAAAGTCACCAATGCCCAACGTGAAGCCACCGTCTCGGTGAGTTTCAACGGCCCGCCGG
ATTATGCCTTTTTCCCACTGCACGGCCCGGACCGCGTGGTCCTGGACGTTAACCAGAAGGGCAAGGTCGGCGGTCTGCCG
CTGAATTTTAGCGGTCAAAACCTGGTGAAAAGTATCCGCTCCAGTACGCCGAAAGACGCCCAGAGTGTGCGGCTGGTGTT
TGACCTGACACAACGTTCGAAGACCCGGGTGGCCACACGCCAAAGCGGCAGTATCTATACCGTGGTGTTTACCATTGCCG
CCGAGGGTAGCGCTAATACCAACATAGTGCGTAAAGCTCCGGTACCTGCGCCGGTGGTGAGCCAGCCAACGCGTAAAGCG
CCAGTCGTGGTGAGTGAGCCTGATGAGCCGGCACCGGTGCGTAAAGCACCAAGCGGTTCGAATCCGTTCAGCAACAAGAC
AACCGTAGTTGCGGGGACCGCATCGGAAATTACGCCGCGCAGCAGCCGCGTTTCCGCAGGTTCCGGCGATCGGGTAGTGG
TAGCCATTGACGCCGGTCACGGCGGGCAGGATCCGGGCGCTATCGGCCCGAACGGCCTGAAAGAGAAGAATGTCACTATC
GCTATTGCTCGTCGCCTGCAGGCGTTGTTGGATGCAGATCCTCAGTTCAAACCGGTCCTGACGCGCAATGGTGACTACTT
CATCTCGGTGATGGGACGTTCCGACGTAGCGCGCAAGCAGGGCGCCAACGTGCTGATCTCCATTCACGCCGATGCCGCGC
CAAACCGCAGTGCCAACGGGGCCTCGGTTTGGGTTCTGTCCAACCGCCGGGCCAACAGTGAAATGGCGGGCTGGCTGGAA
CAGCATGAGAAACAGTCTGAATTGCTGGGCGGTGCGGGCGATTTGCTGGCTAACAGCCAGGCGGATCCTTACCTGAGCCA
GGCAGTGCTGGATCTGCAATTCGGTCACTCGCAGCGTGTGGGTTACGATGTTGCGGTCAAAGTCTTGCAGCAGTTACAGA
GCGTCGGTTCATTGCATAAACGTCGTCCGGAACACGCCAGCCTTGGCGTGCTGCGTTCGCCGGACATTCCATCATTGCTG
GTGGAAACAGGATTTATCAGTAATAGCACGGAGGAGCGGCTGCTCGGCAGTAGCGCGTATCAGGAAAAAATTGCCAGGGC
GATCCACAACGGCCTGCGTAGTTATTTCCTGGCGCATCCGCTACAAGCCGACCCAAAGGTGGAAAACCGACCGCTGGACG
TCGCAGCGGCGGTTAACTCCTCAACACCAGACGTCAGTCAGCCTGCACCGATCGTCAGTTCGGCCGGCAGCAGCCGTATT
AGCGGTAAAACGCAGATTCACGTAGTGAAGCGGGCGGAAACGCTCTCCGGGATCGCCGACAGCTATGGCACTACCATGGC
GGCGTTGCGTGACCTGAACAAGCTGAAGAAGGATGGCGTATGGGTCGGCCAGCGCCTCAAGGTGCCGGCGGGTAAATCCG
CCGCTGCAGCCAGCACGGTCGCCAAGGCGAAACCCAGCGTGAAGAAGCCATCGAAGCATAAAGTGGCCCGCGGTGATACT
TTGACTTCTATTGCCAGCCGTTATGGTGTCAGCGTCAGCGATCTGAAACGCGTGAATAAACTGAAGTCGGACGTCGCGCC
GCTGGATCGGACGCTGACCATTCCGCAGGCCTAG

Upstream 100 bases:

>100_bases
AGCTATCAGGATCAGGGGCGAGAAGCAAAAATTCAGGCGGTTTCCGCCTACGGCAGCCAGCTTTTAGACCGTATTCACGG
GCCACAGGAATGACGCCGCA

Downstream 100 bases:

>100_bases
CGCCCACATACAGGAGTCAGCATGCCCATCCAGGTGTTACCGCCCCAGCTTGCCAACCAGATTGCCGCCGGTGAAGTGGT
CGAGCGGCCCGCGTCGGTGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTYALRKLFVIALIAVLGPLGAASALAASSLSDIKVTNAQREATVSVSFNGPPDYAFFPLHGPDRVVLDVNQKGKVGGLP
LNFSGQNLVKSIRSSTPKDAQSVRLVFDLTQRSKTRVATRQSGSIYTVVFTIAAEGSANTNIVRKAPVPAPVVSQPTRKA
PVVVSEPDEPAPVRKAPSGSNPFSNKTTVVAGTASEITPRSSRVSAGSGDRVVVAIDAGHGGQDPGAIGPNGLKEKNVTI
AIARRLQALLDADPQFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSANGASVWVLSNRRANSEMAGWLE
QHEKQSELLGGAGDLLANSQADPYLSQAVLDLQFGHSQRVGYDVAVKVLQQLQSVGSLHKRRPEHASLGVLRSPDIPSLL
VETGFISNSTEERLLGSSAYQEKIARAIHNGLRSYFLAHPLQADPKVENRPLDVAAAVNSSTPDVSQPAPIVSSAGSSRI
SGKTQIHVVKRAETLSGIADSYGTTMAALRDLNKLKKDGVWVGQRLKVPAGKSAAAASTVAKAKPSVKKPSKHKVARGDT
LTSIASRYGVSVSDLKRVNKLKSDVAPLDRTLTIPQA

Sequences:

>Translated_597_residues
MTYALRKLFVIALIAVLGPLGAASALAASSLSDIKVTNAQREATVSVSFNGPPDYAFFPLHGPDRVVLDVNQKGKVGGLP
LNFSGQNLVKSIRSSTPKDAQSVRLVFDLTQRSKTRVATRQSGSIYTVVFTIAAEGSANTNIVRKAPVPAPVVSQPTRKA
PVVVSEPDEPAPVRKAPSGSNPFSNKTTVVAGTASEITPRSSRVSAGSGDRVVVAIDAGHGGQDPGAIGPNGLKEKNVTI
AIARRLQALLDADPQFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSANGASVWVLSNRRANSEMAGWLE
QHEKQSELLGGAGDLLANSQADPYLSQAVLDLQFGHSQRVGYDVAVKVLQQLQSVGSLHKRRPEHASLGVLRSPDIPSLL
VETGFISNSTEERLLGSSAYQEKIARAIHNGLRSYFLAHPLQADPKVENRPLDVAAAVNSSTPDVSQPAPIVSSAGSSRI
SGKTQIHVVKRAETLSGIADSYGTTMAALRDLNKLKKDGVWVGQRLKVPAGKSAAAASTVAKAKPSVKKPSKHKVARGDT
LTSIASRYGVSVSDLKRVNKLKSDVAPLDRTLTIPQA
>Mature_596_residues
TYALRKLFVIALIAVLGPLGAASALAASSLSDIKVTNAQREATVSVSFNGPPDYAFFPLHGPDRVVLDVNQKGKVGGLPL
NFSGQNLVKSIRSSTPKDAQSVRLVFDLTQRSKTRVATRQSGSIYTVVFTIAAEGSANTNIVRKAPVPAPVVSQPTRKAP
VVVSEPDEPAPVRKAPSGSNPFSNKTTVVAGTASEITPRSSRVSAGSGDRVVVAIDAGHGGQDPGAIGPNGLKEKNVTIA
IARRLQALLDADPQFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSANGASVWVLSNRRANSEMAGWLEQ
HEKQSELLGGAGDLLANSQADPYLSQAVLDLQFGHSQRVGYDVAVKVLQQLQSVGSLHKRRPEHASLGVLRSPDIPSLLV
ETGFISNSTEERLLGSSAYQEKIARAIHNGLRSYFLAHPLQADPKVENRPLDVAAAVNSSTPDVSQPAPIVSSAGSSRIS
GKTQIHVVKRAETLSGIADSYGTTMAALRDLNKLKKDGVWVGQRLKVPAGKSAAAASTVAKAKPSVKKPSKHKVARGDTL
TSIASRYGVSVSDLKRVNKLKSDVAPLDRTLTIPQA

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=457, Percent_Identity=61.2691466083151, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI87082163, Length=422, Percent_Identity=32.4644549763033, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788776, Length=229, Percent_Identity=43.2314410480349, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 63022; Mature: 62891

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYALRKLFVIALIAVLGPLGAASALAASSLSDIKVTNAQREATVSVSFNGPPDYAFFPL
CCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCEEEEEC
HGPDRVVLDVNQKGKVGGLPLNFSGQNLVKSIRSSTPKDAQSVRLVFDLTQRSKTRVATR
CCCCEEEEEECCCCCCCCEECCCCHHHHHHHHHCCCCCCHHHEEEEEEEHHCHHHHHHCC
QSGSIYTVVFTIAAEGSANTNIVRKAPVPAPVVSQPTRKAPVVVSEPDEPAPVRKAPSGS
CCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
NPFSNKTTVVAGTASEITPRSSRVSAGSGDRVVVAIDAGHGGQDPGAIGPNGLKEKNVTI
CCCCCCEEEEEECCHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHH
AIARRLQALLDADPQFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSANG
HHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCCCC
ASVWVLSNRRANSEMAGWLEQHEKQSELLGGAGDLLANSQADPYLSQAVLDLQFGHSQRV
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCC
GYDVAVKVLQQLQSVGSLHKRRPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAY
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCHHH
QEKIARAIHNGLRSYFLAHPLQADPKVENRPLDVAAAVNSSTPDVSQPAPIVSSAGSSRI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCC
SGKTQIHVVKRAETLSGIADSYGTTMAALRDLNKLKKDGVWVGQRLKVPAGKSAAAASTV
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEECCCCCCHHHHHHH
AKAKPSVKKPSKHKVARGDTLTSIASRYGVSVSDLKRVNKLKSDVAPLDRTLTIPQA
HHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCC
>Mature Secondary Structure 
TYALRKLFVIALIAVLGPLGAASALAASSLSDIKVTNAQREATVSVSFNGPPDYAFFPL
CHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCEEEEEC
HGPDRVVLDVNQKGKVGGLPLNFSGQNLVKSIRSSTPKDAQSVRLVFDLTQRSKTRVATR
CCCCEEEEEECCCCCCCCEECCCCHHHHHHHHHCCCCCCHHHEEEEEEEHHCHHHHHHCC
QSGSIYTVVFTIAAEGSANTNIVRKAPVPAPVVSQPTRKAPVVVSEPDEPAPVRKAPSGS
CCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
NPFSNKTTVVAGTASEITPRSSRVSAGSGDRVVVAIDAGHGGQDPGAIGPNGLKEKNVTI
CCCCCCEEEEEECCHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHH
AIARRLQALLDADPQFKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSANG
HHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCCCC
ASVWVLSNRRANSEMAGWLEQHEKQSELLGGAGDLLANSQADPYLSQAVLDLQFGHSQRV
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCC
GYDVAVKVLQQLQSVGSLHKRRPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAY
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCHHH
QEKIARAIHNGLRSYFLAHPLQADPKVENRPLDVAAAVNSSTPDVSQPAPIVSSAGSSRI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCC
SGKTQIHVVKRAETLSGIADSYGTTMAALRDLNKLKKDGVWVGQRLKVPAGKSAAAASTV
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEECCCCCCHHHHHHH
AKAKPSVKKPSKHKVARGDTLTSIASRYGVSVSDLKRVNKLKSDVAPLDRTLTIPQA
HHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]