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The map label for this gene is birA [H]

Identifier: 157368517

GI number: 157368517

Start: 305170

End: 306132

Strand: Direct

Name: birA [H]

Synonym: Spro_0268

Alternate gene names: 157368517

Gene position: 305170-306132 (Clockwise)

Preceding gene: 157368516

Following gene: 157368519

Centisome position: 5.6

GC content: 49.64

Gene sequence:

>963_bases
ATGAAGGACACCAAAGTTCCCCTTAAGCTCATTTCTATACTGGCTGATGGCGATTTCCATTCTGGTGAGCATCTTGGCGA
GTTGCTGGGCATGAGTCGTGCCGCTATTAATAAACATATCCAGACCATCCGGGAATGGGGTTTGGATGTGTTTACCGTGC
CCGGCAAGGGCTATAGCCTGCCGAGCCCGATCCAGTTACTGGATGTTGAATGTATTCTTGACCAACTGGTTGATAAACGT
GTCACGGTGCTGCCGGTTGTGGACTCCACCAACCAATATTTGCTGGATCGTATTACCGAACTGCAATCTGGCGATGCCTG
TGTTGCTGAGTACCAGCAGGCCGGTCGTGGCCGTCGCGGTCGTCAATGGATCTCACCATTTGGCGCCAATCTGTATCTCT
CCATGTTTTGGCGTCTGGATCAGGGGCCTGCAGCCGCAATGGGGCTGAGTCTGGTGATTGGCATCGTGATGGCCGAGGTG
CTCAAAGACCTTGGGGCACAAGATGTCAGGGTTAAATGGCCAAACGACTTATACCTTAACGATCGCAAGTTAGCTGGTAT
CTTGGTCGAATTAACAGGAAAAACCGGTGATGCGGCGCAACTGGTCATTGGTGCCGGTATCAATTTGGCAATGCGTGAAA
CCAATGCCAATGCGATTAATCAAGGCTGGATAAACCTGCAAGAAGCGGGGATTAATATCGATCGTAATGAATTGGCCGCC
ACCTTACTCAATGAATTGCGTCAATCGCTGCGGCAATTTGAGATTGATGGTTTAGCGCCATTTATTGCGCGCTGGCGTGC
TTTGGACAACTTTATCGATCGGCCGGTTAAACTGTTAATTGGTGAACAACAAATTTTTGGTATAGCACGAGGCATTGATC
AACAAGGGGCATTATTGCTCGATCAGGACGGTGTGATTAAACCGTTCATTGGTGGGGAAATCTCTCTACGCAGCGCCGAG
TAA

Upstream 100 bases:

>100_bases
ATGTACGTAATACCGTAGCGAAAAAATTCGACGTATGGCTTGAGCCGGAAGTTCGCTTTATCGGTGCGGTTGGTGAGTTG
AATGCCGTAGAGGTTCTTTC

Downstream 100 bases:

>100_bases
TTATCATTAAATATAAGGGGCCGGTAGGCCCCTGCTTATTATTAAATTATTTTCTTAAACGTACGTTTTCAACCGCGTGA
TTAGCACTCTTCGTCATGAT

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKDTKVPLKLISILADGDFHSGEHLGELLGMSRAAINKHIQTIREWGLDVFTVPGKGYSLPSPIQLLDVECILDQLVDKR
VTVLPVVDSTNQYLLDRITELQSGDACVAEYQQAGRGRRGRQWISPFGANLYLSMFWRLDQGPAAAMGLSLVIGIVMAEV
LKDLGAQDVRVKWPNDLYLNDRKLAGILVELTGKTGDAAQLVIGAGINLAMRETNANAINQGWINLQEAGINIDRNELAA
TLLNELRQSLRQFEIDGLAPFIARWRALDNFIDRPVKLLIGEQQIFGIARGIDQQGALLLDQDGVIKPFIGGEISLRSAE

Sequences:

>Translated_320_residues
MKDTKVPLKLISILADGDFHSGEHLGELLGMSRAAINKHIQTIREWGLDVFTVPGKGYSLPSPIQLLDVECILDQLVDKR
VTVLPVVDSTNQYLLDRITELQSGDACVAEYQQAGRGRRGRQWISPFGANLYLSMFWRLDQGPAAAMGLSLVIGIVMAEV
LKDLGAQDVRVKWPNDLYLNDRKLAGILVELTGKTGDAAQLVIGAGINLAMRETNANAINQGWINLQEAGINIDRNELAA
TLLNELRQSLRQFEIDGLAPFIARWRALDNFIDRPVKLLIGEQQIFGIARGIDQQGALLLDQDGVIKPFIGGEISLRSAE
>Mature_320_residues
MKDTKVPLKLISILADGDFHSGEHLGELLGMSRAAINKHIQTIREWGLDVFTVPGKGYSLPSPIQLLDVECILDQLVDKR
VTVLPVVDSTNQYLLDRITELQSGDACVAEYQQAGRGRRGRQWISPFGANLYLSMFWRLDQGPAAAMGLSLVIGIVMAEV
LKDLGAQDVRVKWPNDLYLNDRKLAGILVELTGKTGDAAQLVIGAGINLAMRETNANAINQGWINLQEAGINIDRNELAA
TLLNELRQSLRQFEIDGLAPFIARWRALDNFIDRPVKLLIGEQQIFGIARGIDQQGALLLDQDGVIKPFIGGEISLRSAE

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=320, Percent_Identity=77.5, Blast_Score=516, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24644246, Length=221, Percent_Identity=26.6968325791855, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35217; Mature: 35217

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTKVPLKLISILADGDFHSGEHLGELLGMSRAAINKHIQTIREWGLDVFTVPGKGYSL
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PSPIQLLDVECILDQLVDKRVTVLPVVDSTNQYLLDRITELQSGDACVAEYQQAGRGRRG
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
RQWISPFGANLYLSMFWRLDQGPAAAMGLSLVIGIVMAEVLKDLGAQDVRVKWPNDLYLN
CHHHCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEC
DRKLAGILVELTGKTGDAAQLVIGAGINLAMRETNANAINQGWINLQEAGINIDRNELAA
CCCEEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHCCCEEEECCCCCCCHHHHHH
TLLNELRQSLRQFEIDGLAPFIARWRALDNFIDRPVKLLIGEQQIFGIARGIDQQGALLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCCCEEE
DQDGVIKPFIGGEISLRSAE
CCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MKDTKVPLKLISILADGDFHSGEHLGELLGMSRAAINKHIQTIREWGLDVFTVPGKGYSL
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PSPIQLLDVECILDQLVDKRVTVLPVVDSTNQYLLDRITELQSGDACVAEYQQAGRGRRG
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
RQWISPFGANLYLSMFWRLDQGPAAAMGLSLVIGIVMAEVLKDLGAQDVRVKWPNDLYLN
CHHHCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEC
DRKLAGILVELTGKTGDAAQLVIGAGINLAMRETNANAINQGWINLQEAGINIDRNELAA
CCCEEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHCCCEEEECCCCCCCHHHHHH
TLLNELRQSLRQFEIDGLAPFIARWRALDNFIDRPVKLLIGEQQIFGIARGIDQQGALLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCCCEEE
DQDGVIKPFIGGEISLRSAE
CCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]