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The map label for this gene is hpaB [H]

Identifier: 157163792

GI number: 157163792

Start: 4584194

End: 4585756

Strand: Reverse

Name: hpaB [H]

Synonym: EcHS_A4576

Alternate gene names: 157163792

Gene position: 4585756-4584194 (Counterclockwise)

Preceding gene: 157163793

Following gene: 157163791

Centisome position: 98.76

GC content: 54.57

Gene sequence:

>1563_bases
ATGAAACCAGAAGATTTCCGCGCCAGTACCCAACGTCCGTTCACCGGGGAAGAGTATCTGAAAAGCCTGCAGGATGGTCG
CGAGATCTATATCTATGGCGAGCGAGTGAAAGACGTCACTACTCATCCGGCATTTCGTAATGCGGCAGCGTCTGTTGCCC
AACTGTACGACGCGCTGCACAAACCGGAGATGCAGGACTCTCTGTGCTGGAACACCGACACCGGCAGCGGCGGCTATACC
CATAAATTCTTCCGCGTGGCGAAAAGTGCCGACGACCTGCGCCAGCAACGCGACGCCATCGCTGAGTGGTCACGCCTGAG
CTATGGCTGGATGGGCCGTACCCCAGACTACAAAGCTGCTTTCGGTTGCGCACTGGGCGCGAATCCGGGCTTTTACGGTC
AGTTCGAGCAGAACGCCCGTAACTGGTACACCCGTATTCAGGAAACTGGCCTCTACTTTAACCACGCGATTGTTAACCCA
CCGATCGATCGTCATTTGCCGACCGATAAAGTAAAAGACGTTTACATCAAGCTGGAAAAAGAGACTGACGCCGGGATTAT
CGTCAGCGGTGCGAAAGTGGTTGCCACCAACTCGGCGCTGACTCACTACAACATGATTGGCTTCGGCTCGGCACAAGTGA
TGGGCGAAAACCCGGACTTCGCACTGATGTTCGTTGCGCCAATGGATGCCGATGGCGTGAAATTAATCTCCCGCGCCTCT
TATGAGATGGTCGCGGGTGCTACCGGCTCGCCATACGACTACCCGCTCTCCAGCCGCTTCGATGAGAACGATGCGATTCT
GGTGATGGATAACGTGCTGATCCCATGGGAAAACGTACTGATCTACCGCGATTTCGATCGCTGCCGTCGCTGGACAATGG
AAGGCGGTTTTGCCCGTATGTATCCGCTGCAAGCCTGTGTGCGCCTGGCAGTGAAACTCGACTTCATTACAGCACTGCTG
AAAAAATCACTCGAATGTACCGGCACCCTGGAGTTCCGTGGTGTACAGGCCGATCTCGGTGAAGTGGTGGCGTGGCGCAA
CACCTTCTGGGCATTGAGTGACTCGATGTGTTCTGAAGCGACGCCGTGGGTCAACGGGGCTTATTTACCGGATCATGCCG
CACTGCAAACCTATCGCGTACTGGCACCAATGGCCTACGCGAAGATCAAAAACATTATCGAACGCAACGTTACCAGTGGC
CTGATCTATCTCCCTTCCAGTGCCCGTGACCTGAACAATCCGCAGATCGACCAGTATCTGGCGAAGTATGTGCGCGGTTC
GAATGGTATGGATCACGTCCAGCGCATCAAGATCCTCAAACTGATGTGGGACGCCATTGGCAGCGAGTTTGGTGGTCGCC
ACGAACTGTATGAAATCAACTACTCCGGTAGCCAGGATGAGATTCGCCTACAGTGTCTGCGCCAGGCACAAAGCTCCGGC
AATATGGACAAGATGATGGCGATGGTTGATCGCTGCCTGTCGGAATACGACCAGAACGGCTGGACTGTGCCGCACTTGCA
CAACAACGACGATATCAACATGCTGGATAAGCTGCTGAAATAA

Upstream 100 bases:

>100_bases
AAAGTGTCTTCCCGTAATCGCCCCTGCAAGCTTCAATTGAATAACAAAAACAACACACAAAATTAACAATCGTATTCCGA
TTAATACTGTAGAGGTCGAC

Downstream 100 bases:

>100_bases
CGCAGCAGGAGGTTAAGATGCAATTAGATGAACAGCGCCTGCGCTTTCGTGACGCGATGGCCAGCCTGTCGGCAGCGGTA
AATATTATCACCACCGAGGG

Product: 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYT
HKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRAS
YEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALL
KKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSG
LIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSG
NMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK

Sequences:

>Translated_520_residues
MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYT
HKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRAS
YEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALL
KKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSG
LIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSG
NMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK
>Mature_520_residues
MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYT
HKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRAS
YEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALL
KKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSG
LIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSG
NMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK

Specific function: Utilizes FADH(2) supplied by hpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012688 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 58865; Mature: 58865

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQLYDALH
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
KPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAA
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRHLPTDKVKDVYIKLEK
HEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEC
ETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRAS
CCCCCEEEECCEEEEECCCHHEEEEEECCCCEEECCCCCEEEEEEECCCCCCHHHHHHCC
YEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARM
HHHEECCCCCCCCCCCCCCCCCCCEEEEECCEECCCCCEEEEECHHHHHHHHCCCCCHHH
YPLQACVRLAVKLDFITALLKKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSEA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
TPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSGLIYLPSSARDLNNPQIDQYL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHH
AKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
NMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHC
>Mature Secondary Structure
MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQLYDALH
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
KPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAA
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNPPIDRHLPTDKVKDVYIKLEK
HEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEC
ETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDADGVKLISRAS
CCCCCEEEECCEEEEECCCHHEEEEEECCCCEEECCCCCEEEEEEECCCCCCHHHHHHCC
YEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARM
HHHEECCCCCCCCCCCCCCCCCCCEEEEECCEECCCCCEEEEECHHHHHHHHCCCCCHHH
YPLQACVRLAVKLDFITALLKKSLECTGTLEFRGVQADLGEVVAWRNTFWALSDSMCSEA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
TPWVNGAYLPDHAALQTYRVLAPMAYAKIKNIIERNVTSGLIYLPSSARDLNNPQIDQYL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHH
AKYVRGSNGMDHVQRIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
NMDKMMAMVDRCLSEYDQNGWTVPHLHNNDDINMLDKLLK
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10653707 [H]