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The map label for this gene is hpaC

Identifier: 157163791

GI number: 157163791

Start: 4583664

End: 4584176

Strand: Reverse

Name: hpaC

Synonym: EcHS_A4575

Alternate gene names: 157163791

Gene position: 4584176-4583664 (Counterclockwise)

Preceding gene: 157163792

Following gene: 157163790

Centisome position: 98.72

GC content: 54.39

Gene sequence:

>513_bases
ATGCAATTAGATGAACAGCGCCTGCGCTTTCGTGACGCGATGGCCAGCCTGTCGGCAGCGGTAAATATTATCACCACCGA
GGGCGACGCCGGACAATGCGGGATTACGGCAACGGCCGTCTGCTCGGTCACGGATACACCACCATCGCTGATGGTGTGCA
TTAACGCCAACAGTGCGATGAACCCGGTTTTTCAGGGCAACGGTAAGTTGTGCGTCAACGTCCTCAACCATGAGCAGGAA
CTGATGGCACGCCACTTCGCGGGCATGACAGGCATGGCGATGGAAGAGCGTTTTAGCCTCTCATGCTGGCAAAAAGGTCC
GCTGGCGCAGCCGGTGCTGAAAGGTTCGCTGGCCAGTCTTGAAGGTGAGATCCGCGATGTGCAGGCAATTGGCACACATC
TGGTGTATCTGGTGGAGATTAAAAACATCATCCTCAGTGCAGAAGGTCACGGACTTATCTACTTTAAACGCCGTTTCCAT
CCGGTGATGCTGGAAATGGAAGCTGCGATTTAA

Upstream 100 bases:

>100_bases
CGGAATACGACCAGAACGGCTGGACTGTGCCGCACTTGCACAACAACGACGATATCAACATGCTGGATAAGCTGCTGAAA
TAACGCAGCAGGAGGTTAAG

Downstream 100 bases:

>100_bases
GCTCCAGCCCCTTCACGCATTCGTCGAAGGGGCATTTTTATTGTGATCTCAATCACATATCCACCCGATGAATAATTCTT
GCCCAACCGCTCACCGCCAT

Product: 4-hydroxyphenylacetate 3-monooxygenase, reductase component

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-monooxygenase small component; Flavin:NADH reductase

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQE
LMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
PVMLEMEAAI

Sequences:

>Translated_170_residues
MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQE
LMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
PVMLEMEAAI
>Mature_170_residues
MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQE
LMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
PVMLEMEAAI

Specific function: Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large hpaB component or to other electron acceptors such as cytochrome c and Fe(3+) ion

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily

Homologues:

Organism=Escherichia coli, GI87081819, Length=161, Percent_Identity=44.7204968944099, Blast_Score=140, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HPAC_ECOLX (Q57501)

Other databases:

- EMBL:   Z29081
- EMBL:   Z37980
- PIR:   C55349
- ProteinModelPortal:   Q57501
- SMR:   Q57501
- BioCyc:   MetaCyc:MONOMER-2842
- InterPro:   IPR002563
- InterPro:   IPR011982
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- SMART:   SM00903
- TIGRFAMs:   TIGR02296

Pfam domain/function: PF01613 Flavin_Reduct; SSF50475 FMN_binding

EC number: 1.14.13.3

Molecular weight: Translated: 18522; Mature: 18522

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAM
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCC
NPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASL
CCEEECCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHCCEECCCCCCCCCHHHHHHHHHH
EGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPVMLEMEAAI
CCHHHHHHHHHHHHHHEEEEEEHEEECCCCEEEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAM
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCC
NPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASL
CCEEECCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHCCEECCCCCCCCCHHHHHHHHHH
EGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPVMLEMEAAI
CCHHHHHHHHHHHHHHEEEEEEHEEECCCCEEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10633095