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The map label for this gene is qseD

Identifier: 157163773

GI number: 157163773

Start: 4562386

End: 4563297

Strand: Reverse

Name: qseD

Synonym: EcHS_A4555

Alternate gene names: 157163773

Gene position: 4563297-4562386 (Counterclockwise)

Preceding gene: 157163774

Following gene: 157163770

Centisome position: 98.27

GC content: 50.77

Gene sequence:

>912_bases
ATGGATGACTGTGGTGCGATTTTGCATAATATTGAAACCAAATGGCTTTATGATTTTCTGACCCTGGAAAAATGCCGCAA
TTTTTCCCAGGCGGCAGTCAGTCGCAACGTCTCGCAACCGGCATTCAGCCGCCGCATCCGTGCGCTGGAACAGGCGATTG
GTGTTGAATTGTTTAACCGCCAAGTAACGCCGCTGCAACTCTCAGAACAAGGAAAAATCTTCCATTCGCAGATCCGCCAT
CTGTTGCAACAGTTAGAGAGCAATCTGGCAGAGCTGCGTGGCGGCAGCGATTACGCGCAACGAAAAATCAAGATAGCCGC
TGCACACTCTCTTTCCCTCGGACTGTTACCGTCCATTATCAGCCAGATGCCGCCGCTCTTTACCTGGGCAATTGAAGCTA
TTGATGTCGATGAAGCGGTCGATAAACTGCGTGAAGGGCAAAGTGACTGTATTTTTTCCTTTCACGACGAAGATTTGCTG
GAAGCGCCGTTTGACCACATTCGCTTATTTGAATCTCAATTGTTCCCTGTCTGCGCCAGTGACGAACACGGAGAAGCACT
TTTTAACCTCGCGCAGCCACACTTTCCGTTACTGAATTACAGCCGCAACTCCTACATGGGGCGATTGATTAATCGCACCC
TGACGCGCCACAGTGAGTTAAGTTTCAGCACCTTTTTTGTCTCTTCGATGAGCGAGCTTTTAAAGCAGGTTGCCCTCGAC
GGCTGTGGGATTGCCTGGCTGCCGGAGTACGCCATCCAACAAGAAATTCGCAGCGGGAAGCTCGTTGTACTGAATCGGGA
CGAACTGGTGATCCCGATTCAGGCCTATGCATACCGAATGAATACCCGCATGAATCCAGTTGCCGAACGCTTCTGGCGTG
AACTGCGCGAGCTGGAGATTGTGCTTAGCTGA

Upstream 100 bases:

>100_bases
AAAGCCTGCGTGAAAGGAACGTTTGAAACGGCTTAAACGGTTGTCAGCCGCCCGAATGGACGTAGAATGCCCGCAAGGGC
GGCTGACAGAGTAAAACGTA

Downstream 100 bases:

>100_bases
CATTCTCCAGCATCGCACTACACCAACCGCCATTATCCCAGACCAGATCAAACGTTAGCGGTTCACCGTCGTGGTACAGC
ATTATCTCAAGGCTGATCGC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: Quorum-sensing regulator protein D

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRH
LLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
GCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWRELRELEIVLS

Sequences:

>Translated_303_residues
MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRH
LLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
GCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWRELRELEIVLS
>Mature_303_residues
MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRH
LLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
GCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWRELRELEIVLS

Specific function: Down-regulates expression of the flagellar regulon, decreasing motility

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790783, Length=303, Percent_Identity=100, Blast_Score=624, Evalue=1e-180,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): QSED_ECOLI (P39376)

Other databases:

- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U15029
- PIR:   S56552
- RefSeq:   AP_004819.1
- RefSeq:   NP_418747.1
- ProteinModelPortal:   P39376
- DIP:   DIP-12633N
- IntAct:   P39376
- STRING:   P39376
- EnsemblBacteria:   EBESCT00000000056
- EnsemblBacteria:   EBESCT00000014930
- GeneID:   948852
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW4290
- KEGG:   eco:b4327
- EchoBASE:   EB2454
- EcoGene:   EG12566
- eggNOG:   COG0583
- GeneTree:   EBGT00050000008333
- HOGENOM:   HBG625160
- OMA:   LRNDGLN
- ProtClustDB:   PRK10082
- BioCyc:   EcoCyc:G7924-MONOMER
- Genevestigator:   P39376
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34711; Mature: 34711

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNR
CCCHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
QVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSII
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHHHHHHHHHHHH
SQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCAS
HHCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCHHHHCCHHHHHHHHHHCCCCCCC
DEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWRELRELEI
CCCCHHCHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VLS
HCC
>Mature Secondary Structure
MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNR
CCCHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
QVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSII
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEHHHHHHHHHHHHHH
SQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCAS
HHCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCHHHHCCHHHHHHHHHHCCCCCCC
DEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQVALD
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWRELRELEI
CCCCHHCHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VLS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7876157