Click here to switch to the map view.

The map label for this gene is sgbH

Identifier: 157163059

GI number: 157163059

Start: 3777880

End: 3778542

Strand: Direct

Name: sgbH

Synonym: EcHS_A3785

Alternate gene names: 157163059

Gene position: 3777880-3778542 (Clockwise)

Preceding gene: 157163058

Following gene: 157163060

Centisome position: 81.36

GC content: 59.43

Gene sequence:

>663_bases
ATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCGCGACGTGACGCTGTTAAAAGA
CAGCGTCGATATCGTCGAAGCGGGCACCATTCTCTGTTTAAACGAAGGGCTTGGCGCGGTGAAAGCCTTGCGCGAACAGT
GCCCGGACAAAATCATCGTTGCTGACTGGAAGGTCGCCGACGCTGGTGAAACGCTCGCGCAACAGGCGTTTGGCGCAGGC
GCTAACTGGATGACCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGG
TGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTGCGGCAGGCCA
TTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCA
GATATCGGCCTTGAGCTTTCCATTACTGGCGGGATTACCCCTGCTGACCTGCCGCTGTTTAAAGATATCCGCGTGAAAGC
GTTTATTGCCGGGCGGGCACTGGCAGGCGCGGCAAATCCGGCGCAAGTGGCTGGCGATTTCCATGCGCAAATCGACGCTA
TCTGGGGAGGTGCGCGTGCGTAA

Upstream 100 bases:

>100_bases
ATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCC
CGCATTAAGGAGCACACATT

Downstream 100 bases:

>100_bases
TCATCCGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCG
GTTTTGATTTTGTCGAAATG

Product: 3-keto-L-gulonate-6-phosphate decarboxylase

Products: NA

Alternate protein names: KGPDC; 3-dehydro-L-gulonate-6-phosphate decarboxylase

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGLGAVKALREQCPDKIIVADWKVADAGETLAQQAFGAG
ANWMTIICAAPLATVEKGHAMAQRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGARA

Sequences:

>Translated_220_residues
MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGLGAVKALREQCPDKIIVADWKVADAGETLAQQAFGAG
ANWMTIICAAPLATVEKGHAMAQRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGARA
>Mature_219_residues
SRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGLGAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGA
NWMTIICAAPLATVEKGHAMAQRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALSD
IGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGARA

Specific function: Catalyzes the decarboxylation of 3-keto-L-gulonate-6-P into L-xylulose-5-P. May be involved in the utilization of 2,3- diketo-L-gulonate

COG id: COG0269

COG function: function code G; 3-hexulose-6-phosphate synthase and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPS/KGPDC family. KGPDC subfamily

Homologues:

Organism=Escherichia coli, GI1790006, Length=220, Percent_Identity=100, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790640, Length=216, Percent_Identity=45.8333333333333, Blast_Score=209, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SGBH_ECOLI (P37678)

Other databases:

- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S47802
- RefSeq:   AP_004211.1
- RefSeq:   NP_418038.1
- ProteinModelPortal:   P37678
- SMR:   P37678
- DIP:   DIP-10873N
- IntAct:   P37678
- MINT:   MINT-1281843
- STRING:   P37678
- EnsemblBacteria:   EBESCT00000003839
- EnsemblBacteria:   EBESCT00000003840
- EnsemblBacteria:   EBESCT00000016930
- GeneID:   948098
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3553
- KEGG:   eco:b3581
- EchoBASE:   EB2193
- EcoGene:   EG12285
- eggNOG:   COG0269
- GeneTree:   EBGT00050000008924
- HOGENOM:   HBG417610
- OMA:   ILCISAG
- ProtClustDB:   PRK13305
- BioCyc:   EcoCyc:EG12285-MONOMER
- BioCyc:   MetaCyc:EG12285-MONOMER
- Genevestigator:   P37678
- InterPro:   IPR013785
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.85

Molecular weight: Translated: 23445; Mature: 23314

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: BINDING 11-11 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGLGAVKALREQCPDKIIV
CCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHCCCCEEE
ADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMAQRCGGEIQIELFGNWTLD
EEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
DARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALSDIGLELSITGGITPADLPLF
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHH
KDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGARA
HHCEEEEEEECHHHCCCCCHHHHCCCHHHEEEEECCCCCC
>Mature Secondary Structure 
SRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGLGAVKALREQCPDKIIV
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHCCCCEEE
ADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMAQRCGGEIQIELFGNWTLD
EEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
DARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALSDIGLELSITGGITPADLPLF
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHH
KDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGARA
HHCEEEEEEECHHHCCCCCHHHHCCCHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9274005; 11741871