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The map label for this gene is treF

Identifier: 157162998

GI number: 157162998

Start: 3710904

End: 3712553

Strand: Direct

Name: treF

Synonym: EcHS_A3722

Alternate gene names: 157162998

Gene position: 3710904-3712553 (Clockwise)

Preceding gene: 157162992

Following gene: 157163000

Centisome position: 79.92

GC content: 53.76

Gene sequence:

>1650_bases
ATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTA
TGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCCGCCTCTGATGCGCTGA
CGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGAC
TGCGCACCCAAAATGGACCCGCTGGATATTTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA
GTTTGTTGAAAATCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGC
ATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACAGGATCACATTCCGTGGTCTTCTCTACTGGCGCTGCCGCAG
TCATATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATTGGGACTCCTATTTCACCATGCTGGGGCTGGCGGAAAG
TGGTCGGGAAGATTTGCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTATGGTCACATCCCCAACGGCA
ACCGCACCTATTATTTGAGCCGATCGCAACCACCGGTTTTTGCGCTGATGGTGGAGTTGTTTGAAGAAGATGGTGTACGC
GGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTGATCCCTAATCA
GGCCTATCGCCATGTTGTGCGGATGCCGGACGGATCGCTGCTCAACCGTTATTGGGACGATCGCGACACGCCGCGTGACG
AATCCTGGCTTGAGGACGTTGAAACCGCGAAACATTCTGGTCGCCCGCCCAACGAGGTGTACCGCGATTTACGCGCGGGA
GCGGCCTCAGGTTGGGATTACTCTTCCCGTTGGCTGCGTGATACTGGTCGTCTGGCGAGCATTCGTACCACCCAGTTCAT
CCCCATCGATCTGAATGCCTTCCTGTTTAAACTGGAGAGCGCCATCGCCAACATCTCGGCGCTGAAAGGCGAGAAAGAGA
CAGAAGCGCTGTTCCGCCAGAAGGCCAGTGCCCGTCGCGATGCGGTAAACCGTTACCTCTGGGATGATGAAAACGGCATC
TACCGCGATTACGACTGGCGACGCGAACAACTGGCGCTGTTTTCCGCTGCCGCCATTGTGCCGCTCTATGTCGGCATGGC
GAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTACGCAGCCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGT
ACGAAACCGGTGAACAGTGGGATAAACCCAATGGCTGGGCACCGTTACAATGGATGGCAATTCAGGGATTTAAAATGTAT
GGCGATGACCTTCTGGGTGATGAAATCGCGCGCAGCTGGCTGAAAACGGTGAATCAGTTCTATCTGGAACAGCACAAAAT
GATCGAGAAATACCATATTGCCGATGGTGTTCCCCGCGAAGGCGGCGGTGGCGAGTATCCGTTGCAGGATGGGTTTGGCT
GGACTAACGGTGTGGTACGCCGTTTAATTGGTTTGTACGGCGAACCATAA

Upstream 100 bases:

>100_bases
CGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCCGATAATTAGCGACGA
ATTTCGGAGGTTGGATCCTT

Downstream 100 bases:

>100_bases
TATTTTTACAGCCAGCCGCTAACTTCCTGCTGGCTGTAAAATTATCCTCTTCAGGAGGAGATATTTAACATCATTGCCGC
CTGGGTGCGATTTTTCACTT

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Sequences:

>Translated_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP
>Mature_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Specific function: Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family

Homologues:

Organism=Homo sapiens, GI116284412, Length=525, Percent_Identity=34.2857142857143, Blast_Score=255, Evalue=7e-68,
Organism=Escherichia coli, GI1789936, Length=549, Percent_Identity=99.8178506375228, Blast_Score=1130, Evalue=0.0,
Organism=Escherichia coli, GI1787447, Length=487, Percent_Identity=50.9240246406571, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17542196, Length=527, Percent_Identity=31.8785578747628, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI25141398, Length=529, Percent_Identity=31.5689981096408, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17565078, Length=510, Percent_Identity=31.5686274509804, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI25148109, Length=546, Percent_Identity=30.2197802197802, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71987755, Length=570, Percent_Identity=28.0701754385965, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320204, Length=473, Percent_Identity=27.6955602536998, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319473, Length=481, Percent_Identity=28.8981288981289, Blast_Score=143, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24656680, Length=550, Percent_Identity=31.4545454545455, Blast_Score=233, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24656675, Length=550, Percent_Identity=31.4545454545455, Blast_Score=233, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24656661, Length=535, Percent_Identity=31.588785046729, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17933716, Length=535, Percent_Identity=31.588785046729, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24656670, Length=535, Percent_Identity=31.588785046729, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24656685, Length=486, Percent_Identity=32.0987654320988, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI22024178, Length=533, Percent_Identity=26.078799249531, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI45551104, Length=376, Percent_Identity=26.3297872340426, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28573474, Length=297, Percent_Identity=26.9360269360269, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TREF_ECO24 (A7ZT60)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001464989.1
- ProteinModelPortal:   A7ZT60
- SMR:   A7ZT60
- STRING:   A7ZT60
- EnsemblBacteria:   EBESCT00000021105
- GeneID:   5586140
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4007
- eggNOG:   COG1626
- GeneTree:   EBGT00050000010574
- HOGENOM:   HBG485982
- OMA:   FWMDGAD
- ProtClustDB:   PRK13270
- BioCyc:   ECOL331111:ECE24377A_4007-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01059
- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232
- PANTHER:   PTHR23403
- PRINTS:   PR00744

Pfam domain/function: PF01204 Trehalase; SSF48208 Glyco_trans_6hp

EC number: =3.2.1.28

Molecular weight: Translated: 63715; Mature: 63715

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00927 TREHALASE_1; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC
>Mature Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA