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The map label for this gene is arsB [H]

Identifier: 157162980

GI number: 157162980

Start: 3691446

End: 3692735

Strand: Direct

Name: arsB [H]

Synonym: EcHS_A3704

Alternate gene names: 157162980

Gene position: 3691446-3692735 (Clockwise)

Preceding gene: 157162979

Following gene: 157162981

Centisome position: 79.5

GC content: 53.95

Gene sequence:

>1290_bases
ATGTTACTGGCAGGCGCTATCTTTGTCCTGACCATCGTATTGGTTATCTGGCAGCCGAAAGGTTTAGGCATCGGCTGGAG
TGCAACGCTCGGCGCAGTACTGGCGTTAGTTACGGGCGTGGTCCATCCGGGTGATATTCCGGTGGTGTGGAATATCGTCT
GGAACGCGACGGCTGCGTTTATCGCCGTCATTATCATCAGCCTGCTGCTGGATGAGTCCGGCTTTTTTGAATGGGCGGCG
CTGCACGTCTCACGCTGGGGTAATGGTCGTGGTCGCTTGCTGTTTACCTGGATTGTCCTGCTCGGTGCTGCCGTTGCCGC
CCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAG
GCACTACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCACTTATTGTCTCCAACCTGGTG
AATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCTCGGTGATGGTGCCGGTGGATATCGCCGCGAT
TGTTGCCACGCTGGTGATGCTACATCTCTATTTTCGCAAAGATATTCCGCAGAACTACGATATGGCGCTGCTGAAATCTC
CCGCAGAAGCGATCAAAGATCCTGCTACGTTCAAAACTGGCTGGGTTGTTTTACTGCTTCTGCTGGTGGGATTTTTCGTC
CTGGAACCGCTCGGCATTCCGGTGAGCGCCATTGCAGCTGTGGGCGCGCTGATATTATTTGTCGTCGCTAAACGCGGTCA
TGCGATTAATACGGGTAAAGTCCTGCGCGGTGCCCCCTGGCAGATTGTCATCTTCTCGCTCGGCATGTATCTGGTGGTTT
ATGGCCTGCGCAATGCCGGATTAACGGAATATCTTTCTGGCGTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCG
ACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTC
CATTGATGGCAGCACGGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGA
AAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACTATCAGCTGGGGA
TATTACTTCCGTACAGGGATTATCATGACCCTGCCTGTGCTGTTTGTGACGCTGGCTGCGCTGGCGCTACGTCTCTCTTT
CACTTTGTAA

Upstream 100 bases:

>100_bases
TGGCTCGACAAAACTGTTCCGGGGACAGTAAGAACATTTGCAGTTAAAAATTTAGCTAAACACATATGAATTTTCAGATG
TGTTTTATCCGGGAGGCATT

Downstream 100 bases:

>100_bases
TGAGATACTGATATGAGCAACATTACCATTTATCACAACCCGGCCTGCGGCACGTCGCGTAATACGCTGGAGATGATCCG
CAACAGCGGCACAGAACCGA

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTL

Sequences:

>Translated_429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTL
>Mature_429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTL

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=429, Percent_Identity=100, Blast_Score=830, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45498; Mature: 45498

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAF
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEA
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLSFTL
HHHHHHCCC
>Mature Secondary Structure
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAF
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEA
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLSFTL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]