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The map label for this gene is kefG [H]

Identifier: 157162828

GI number: 157162828

Start: 3524950

End: 3525504

Strand: Reverse

Name: kefG [H]

Synonym: EcHS_A3548

Alternate gene names: 157162828

Gene position: 3525504-3524950 (Counterclockwise)

Preceding gene: 157162833

Following gene: 157162827

Centisome position: 75.92

GC content: 56.58

Gene sequence:

>555_bases
ATGATGTCTCAGCCAGCGAAAGTTTTGCTGCTGTATGCCCATCCGGAATCTCAGGACTCGGTGGCAAACCGGGTACTGCT
TAAACCGGCCACGCAGCTCAGCAATGTTACCGTGCACGACCTTTACGCGCACTATCCCGATTTTTTTATTGATATCCCCC
GTGAGCAGGCATTACTGCGCGAGCACGAGGTGATTGTCTTTCAGCATCCTCTTTATACCTATAGCTGCCCGGCGCTACTG
AAAGAGTGGCTGGACCGGGTATTAAGTCGTGGTTTTGCCAGCGGGCCGGGAGGAAACCAACTGGCGGGAAAGTACTGGCG
TAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGC
GCCCCTTTGAACTGGCGGCGGGCATGTGCCGGATGCATTGGTTAAGTCCCATCATTATTTACTGGGCGAGACGGCAAAGC
GCACAGGAGCTGGCGAGCCACGCCAGAGCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGA

Upstream 100 bases:

>100_bases
ACTTATCATTATGATAATGTAATGTATGGGCGAGCTGCCGCACCGGGCGCAATGGTAGCCCAAAACAGCGACTATACACA
AAAACCATACCGGGAGGGGG

Downstream 100 bases:

>100_bases
TGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATCGCGGCTG
GGTATTGGCGCTGTGTTGGG

Product: glutathione-regulated potassium-efflux system ancillary protein KefG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MMSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHYPDFFIDIPREQALLREHEVIVFQHPLYTYSCPALL
KEWLDRVLSRGFASGPGGNQLAGKYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQS
AQELASHARAYGDWLANPLSPGGR

Sequences:

>Translated_184_residues
MMSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHYPDFFIDIPREQALLREHEVIVFQHPLYTYSCPALL
KEWLDRVLSRGFASGPGGNQLAGKYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQS
AQELASHARAYGDWLANPLSPGGR
>Mature_184_residues
MMSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHYPDFFIDIPREQALLREHEVIVFQHPLYTYSCPALL
KEWLDRVLSRGFASGPGGNQLAGKYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQS
AQELASHARAYGDWLANPLSPGGR

Specific function: Required for full activity of kefB [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefG subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789750, Length=184, Percent_Identity=100, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1786231, Length=165, Percent_Identity=40.6060606060606, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 20958; Mature: 20958

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHYPDFFIDIPREQALLR
CCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCHHEEECCHHHHHHH
EHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAGKYWRSVITTGEPESAYR
HCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
YDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSAQELASHARAYGDWLANPLS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGGR
CCCC
>Mature Secondary Structure
MMSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHYPDFFIDIPREQALLR
CCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCHHEEECCHHHHHHH
EHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAGKYWRSVITTGEPESAYR
HCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
YDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSAQELASHARAYGDWLANPLS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGGR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA