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The map label for this gene is kefB [H]

Identifier: 157162827

GI number: 157162827

Start: 3523145

End: 3524950

Strand: Reverse

Name: kefB [H]

Synonym: EcHS_A3547

Alternate gene names: 157162827

Gene position: 3524950-3523145 (Counterclockwise)

Preceding gene: 157162828

Following gene: 157162826

Centisome position: 75.91

GC content: 54.98

Gene sequence:

>1806_bases
ATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATCGCGGCT
GGGTATTGGCGCTGTGTTGGGATATTTGCTGGCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCG
ATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTT
TGGCAACTGCGGCGTTCGATTTTTGGCGTAGGCGCGGCACAGGTGCTGTTAAGCGCGGCGTTGCTGGCGGGATTATTGAT
GCTGACGGATTTCGCCTGGCAGGCGGCGGTGGTCGGTGGCATTGGCCTTGCGATGTCTTCAACTGCAATGGCGTTGCAAT
TGATGCGTGAGAAAGGGATGAATCGCAGCGAATCCGGCCAGTTAGGTTTCTCGGTACTGCTGTTTCAGGATCTGGCGGTG
ATCCCGGCACTGGCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGATCGGCATGAAGGT
GCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTGCAGCTTCTGGCGTGC
GGGAAGTGTTCACCGCCGCGACGCTGCTGCTGGTGTTGGGTTCCGCATTGTTTATGGATGCGCTGGGGCTGTCGATGGCG
CTCGGTACATTTATTGCGGGCGTGCTGCTGGCGGAAAGTGAATATCGCCATGAACTGGAAACGGCTATCGATCCCTTTAA
AGGCTTGCTGCTCGGTTTGTTCTTTATCTCTGTCGGCATGTCGCTCAATCTCGGGGTGCTTTATACCCATCTGTTGTGGG
TAGTGATAAGCGTGGTTGTGCTGGTGGCGGTGAAAATTCTCGTGTTGTATCTGCTGGCGCGATTGTATGGCGTGCGTAGC
TCAGAGCGGATGCAGTTTGCTGGCGTGTTGAGTCAGGGCGGCGAGTTTGCCTTTGTCCTCTTTTCTACCGCTTCTTCGCA
ACGCTTATTCCAGGGCGACCAGATGGCGCTGTTGCTGGTGACGGTGACGCTTTCCATGATGACCACGCCGTTGCTGATGA
AGCTGGTGGATAAATGGCTATCCCGCCAGTTTAACGGACCGGAAGAAGAAGACGAAAAACCGTGGGTCAACGATGATAAA
CCCCAGGTCATTGTCGTGGGCTTCGGGCGTTTTGGTCAGGTGATTGGTCGTTTGCTGATGGCAAATAAAATGCGCATTAC
CGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGCGACGCCACGCAGG
TCGATCTTTTACGTTCTGCGGGTGCAGAGGCCGCTGAGTCTATCGTGATCACCTGTAACGAACCGGAAGACACCATGAAG
CTGGTGGAAATATGCCAGCAGCACTTTCCGCATTTGCATATTCTTGCGCGAGCGCGCGGACGTGTGGAAGCGCATGAGTT
ATTACAGGCAGGGGTGACGCAGTTTTCCCGTGAAACATTCTCCAGTGCGTTAGAGCTGGGGCGCAAGACGCTGGTCACGC
TTGGCATGCATCCGCATCAGGCGCAGCGGGCGCAACTGCATTTTCGCCGCCTGGATATGCGGATGCTGCGAGAGCTCATC
CCAATGCATGCCGATACTGTACAAATTTCTCGCGCCAGGGAAGCCCGACGCGAACTGGAAGAGATTTTCCAGCGTGAAAT
GCAACAAGAACGACGCCAGCTGGACGGCTGGGATGAATTTGAGTAG

Upstream 100 bases:

>100_bases
TTATTTACTGGGCGAGACGGCAAAGCGCACAGGAGCTGGCGAGCCACGCCAGAGCCTACGGTGACTGGCTGGCAAATCCG
CTGTCTCCAGGAGGCCGCTG

Downstream 100 bases:

>100_bases
AGGGTAAAGATGGCAATCCGAAAACGTTTTATTGCGGGCGCAAAATGCCCGGCCTGTCAGGCACAGGATTCAATGGCGAT
GTGGCGCGAAAATAATATTG

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKL
WQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRS
SERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDK
PQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMK
LVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELI
PMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE

Sequences:

>Translated_601_residues
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKL
WQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRS
SERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDK
PQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMK
LVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELI
PMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE
>Mature_601_residues
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKL
WQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRS
SERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDK
PQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMK
LVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELI
PMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=358, Percent_Identity=26.8156424581006, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI1789749, Length=601, Percent_Identity=99.8336106489185, Blast_Score=1198, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=607, Percent_Identity=43.8220757825371, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1786685, Length=527, Percent_Identity=30.1707779886148, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66426; Mature: 66426

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LVAVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCHHHHHHH
TVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVVGFGRFGQVIGRLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
ANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMK
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCHHHCCEEEEECCCHHHHH
LVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQ
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCH
AQRAQLHFRRLDMRMLRELIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
E
C
>Mature Secondary Structure
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LVAVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCHHHHHHH
TVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVVGFGRFGQVIGRLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
ANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMK
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCHHHCCEEEEECCCHHHHH
LVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQ
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCH
AQRAQLHFRRLDMRMLRELIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA