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The map label for this gene is aaeR

Identifier: 157162721

GI number: 157162721

Start: 3433108

End: 3434037

Strand: Direct

Name: aaeR

Synonym: EcHS_A3432

Alternate gene names: 157162721

Gene position: 3433108-3434037 (Clockwise)

Preceding gene: 157162716

Following gene: 157162729

Centisome position: 73.93

GC content: 52.8

Gene sequence:

>930_bases
ATGGAACGACTAAAACGCATGTCGGTGTTTGCCAAAGTAGTTGAATTTGGCTCTTTTACCGCCGCCGCCAGACAGCTACA
GATGAGCGTTTCGTCCATCAGTCAGACGGTGTCAAAACTGGAAGATGAGTTGCAGGTAAAGCTGTTAAACCGTAGTACAC
GCAGCATTGGTCTGACTGAAGCCGGCAGAATTTACTACCAGGGCTGCCGTCGGATGCTCCATGAAGTGCAGGATGTTCAT
GAGCAACTGTATGCCTTCAATAACACCCCCATCGGGACGCTGCGCATTGGCTGTTCTTCAACTATGGCACAAAATGTTCT
CGCCGGGCTGACAGCAAAAATGCTGAAAGAATACCCAGGTTTGAGCGTCAATCTGGTTACCGGAATTCCTGCCCCCGACC
TGATTGCCGATGGTCTGGATGTGGTAATCCGCGTCGGCGCGTTGCAGGATTCCAGCCTGTTTTCCCGCCGTCTGGGCGCG
ATGCCGATGGTGGTGTGCGCCGCGAAAAGCTATCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCA
TTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCC
CACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCG
CTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTACCAGTCAGATCCACGCCCGGT
TTATGCGTTGTATACCGAAAAAGATAAACTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTATTTTGTTG
AGGTCGGTAAATTGTTTCAGGAGATGCATGGGCGCGGGAAAGAGAAGTAA

Upstream 100 bases:

>100_bases
AGAGCAACATTGCTGTAGATTGATATTTAATATATTAGCGTAACTGTTATGCTGTTATCTATATTATGTGATCTAAATCA
CTTTTAAGTCAGAGTGAATA

Downstream 100 bases:

>100_bases
TTCATATTGTACTGTTACGTTGTTCAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGG
GACCGTTCGTGCACGTAGAA

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGAGIAYVP
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK

Sequences:

>Translated_309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGAGIAYVP
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK
>Mature_309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGAGIAYVP
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK

Specific function: Activates transcription of the aaeXAB operon

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789639, Length=309, Percent_Identity=100, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=30.7692307692308, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=31.5972222222222, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787128, Length=304, Percent_Identity=27.3026315789474, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=29.6928327645051, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=28.1021897810219, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=27.2413793103448, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI157672245, Length=121, Percent_Identity=33.0578512396694, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788481, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=26, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=126, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788706, Length=141, Percent_Identity=29.7872340425532, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788297, Length=123, Percent_Identity=30.0813008130081, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786508, Length=147, Percent_Identity=27.891156462585, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): AAER_ECO57 (P67664)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91143
- RefSeq:   NP_289811.1
- RefSeq:   NP_312143.1
- ProteinModelPortal:   P67664
- SMR:   P67664
- EnsemblBacteria:   EBESCT00000024575
- EnsemblBacteria:   EBESCT00000059652
- GeneID:   916027
- GeneID:   958678
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4602
- KEGG:   ecs:ECs4116
- GeneTree:   EBGT00050000008331
- HOGENOM:   HBG685425
- OMA:   KVQVCIN
- ProtClustDB:   PRK10632
- BioCyc:   ECOL83334:ECS4116-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34517; Mature: 34517

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
LSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGAMPMVVCAAKSYLTQYGIPEK
CEEEEEECCCCHHHHHCCHHHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGAGIAYVP
CCCCCCCCEEEEEECCCCCEEEECCCCCCEEECCCCCEECCCHHHHHHHHHHCCCHHHHH
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQ
HHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
EMHGRGKEK
HHHCCCCCC
>Mature Secondary Structure
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKMLKEYPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
LSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGAMPMVVCAAKSYLTQYGIPEK
CEEEEEECCCCHHHHHCCHHHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGAGIAYVP
CCCCCCCCEEEEEECCCCCEEEECCCCCCEEECCCCCEECCCHHHHHHHHHHCCCHHHHH
LMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTDYFVEVGKLFQ
HHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
EMHGRGKEK
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796