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The map label for this gene is agaI [H]

Identifier: 157162626

GI number: 157162626

Start: 3337195

End: 3337950

Strand: Direct

Name: agaI [H]

Synonym: EcHS_A3333

Alternate gene names: 157162626

Gene position: 3337195-3337950 (Clockwise)

Preceding gene: 157162625

Following gene: 157162627

Centisome position: 71.87

GC content: 50.13

Gene sequence:

>756_bases
ATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAA
CTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGGATGCCGTGATTTGCCTGG
CGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCCACCAGCAGCAGGTTGATGTCAGCCAGCTC
ACCTTCGTGAAGCTCGACGAATGGGTGGATCTGCCATTAACGATGCCTAGTACCTGCGAAACTTTCCTGCAACAGCATAT
CGTGCAGCCGCTGGGGCTACGTGAAGACCAGCTCATCAGCTTTCGCTCCGAAGAGATAAATGAGACAGAGTGCGAACGGG
TAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAAC
GAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAC
CGCGGGTCGCCCCGTGACTCGTGGGATCACCTTAGGCCTGAAGGATATTCTCAATGCCCGCGAAGTTTTGTTACTGGTGA
CTGGCGAAGGAAAGCAGGATGCGACAGATCGTTTTCTCACGGCTAAAGTCTCTACCGCTATCCCGGCTTCATTTTTATGG
CTGCACAGTAACTTTATTTGTTTAATTAACACCTAA

Upstream 100 bases:

>100_bases
GATGTATTACTTCCTGCGGGTGAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTT
CCGCATTCGGCATTTTGTAA

Downstream 100 bases:

>100_bases
CCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGT
TGAACATAACGCCGATAGCA

Product: galactosamine-6-phosphate isomerase

Products: D-Tagatose 6-Phosphate; NH3. [C]

Alternate protein names: Galactosamine-6-phosphate deaminase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQL
TFVKLDEWVDLPLTMPSTCETFLQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGKQDATDRFLTAKVSTAIPASFLW
LHSNFICLINT

Sequences:

>Translated_251_residues
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQL
TFVKLDEWVDLPLTMPSTCETFLQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGKQDATDRFLTAKVSTAIPASFLW
LHSNFICLINT
>Mature_251_residues
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQL
TFVKLDEWVDLPLTMPSTCETFLQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGKQDATDRFLTAKVSTAIPASFLW
LHSNFICLINT

Specific function: N-acetylgalactosamine utilization. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=239, Percent_Identity=26.3598326359833, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI13027378, Length=238, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1789530, Length=251, Percent_Identity=99.203187250996, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1786893, Length=241, Percent_Identity=27.8008298755187, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI48994958, Length=233, Percent_Identity=26.1802575107296, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=225, Percent_Identity=28.4444444444444, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581960, Length=238, Percent_Identity=24.7899159663866, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920764, Length=238, Percent_Identity=24.7899159663866, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: 5.3.1.- [C]

Molecular weight: Translated: 27755; Mature: 27755

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
LTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPSTCETFLQQHIVQPLGLREDQLIS
HHHHHHHHHHHHHHCCHHHHHEEEHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
FRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLNEPGESLQPACHISQLDARTQ
HHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGKQDATDRFLTAKVSTAIPASFLW
HHHHHHHCCCCCHHHHHCCHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHE
LHSNFICLINT
EECCEEEEEEC
>Mature Secondary Structure
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
LTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPSTCETFLQQHIVQPLGLREDQLIS
HHHHHHHHHHHHHHCCHHHHHEEEHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
FRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLNEPGESLQPACHISQLDARTQ
HHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGKQDATDRFLTAKVSTAIPASFLW
HHHHHHHCCCCCHHHHHCCHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHE
LHSNFICLINT
EECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Galactosamine 6-Phosphate; H2O [C]

Specific reaction: D-Galactosamine 6-Phosphate + H2O = D-Tagatose 6-Phosphate + NH3. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931310; 9278503; 8932697 [H]