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The map label for this gene is pitB [H]

Identifier: 157162467

GI number: 157162467

Start: 3179702

End: 3181201

Strand: Reverse

Name: pitB [H]

Synonym: EcHS_A3167

Alternate gene names: 157162467

Gene position: 3181201-3179702 (Counterclockwise)

Preceding gene: 157162468

Following gene: 157162465

Centisome position: 68.51

GC content: 52.13

Gene sequence:

>1500_bases
ATGCTAAATTTATTTGTTGGCCTTGATATATACACAGGGCTTTTGTTATTGCTTGCTCTGGCATTTGTGTTGTTCTACGA
AGCAATCAATGGTTTTCATGACACGGCGAATGCGGTGGCAACCGTTATTTATACTCGTGCCATGCAACCACAACTTGCTG
TGGTGATGGCGGCATTTTTTAACTTTTTTGGCGTGTTATTGGGCGGACTTAGCGTTGCCTATGCCATTGTCCATATGTTG
CCAACCGATTTGTTGCTGAATATGGGGTCAACCCACGGCCTGGCGATGGTCTTTTCCATGCTGCTGGCGGCGATTATCTG
GAACCTGGGAACGTGGTTCTTCGGTTTACCGGCCTCCAGTTCGCACACCTTGATTGGTGCGATTATCGGCATCGGTTTAA
CCAACGCGCTGTTAACCGGCTCATCGGTGATGGATGCGTTAAACCTGCGTGAAGTGACCAAAATTTTCTCCTCGCTGATT
GTTTCCCCTATCGTCGGCCTGGTCATTGCGGGAGGCCTGATATTCCTGCTGCGACGCTACTGGAGCGGGACGAAAAAGCG
TGACCGTATTCACCGCATTCCGGAAGATCGCAAAAAGAAAAAAGGCAAACGTAAACCGCCATTCTGGACGCGTATTGCGC
TGATTGTTTCCGCTGCGGGCGTGGCGTTTTCGCACGGCGCGAACGACGGACAAAAAGGGATCGGCCTGGTAATGCTGGTA
CTGGTGGGGATTGCCCCTGCTGGCTTCGTCGTCAATATGAATGCGTCCGGCTATGAAATTACCCGTACCCGCGATGCCGT
TACCAACTTCGAACACTACCTGCAACAGCATCCTGAACTGCCGCAGAAGTTGATTGCGATGGAACCTCCATTGCCTGCAG
CATCGACTGATGGCACGCAAGTAACAGAGTTTCACTGTCATCCGGCAAATACCTTTGATGCTATTGCGCGCGTTAAAACG
ATGCTGCCAGGCAATATGGAAAGTTACGAGCCGTTAACCGTGAGTCAGCGCAGCCAGCTGCGCCGCATTATGCTGTGCAT
CTCTGATACCTCCGCGAAGCTGGCGAAACTGCCAGGCGTCAGTAAAGAAGACCAGAACCTGCTGAAAAAACTGCGCAGCG
ATATGTTAAGCACCATTGAGTACGCTCCGGTGTGGATCATCATGGCGGTAGCACTGGCGCTCGGCATTGGCACCATGATT
GGCTGGCGTCGTGTAGCGATGACCATCGGTGAGAAGATTGGTAAGCGCGGCATGACGTATGCGCAAGGCATGGCGGCACA
AATGACGGCGGCAGTGTCTATCGGCCTTGCCAGTTATATTGGGATGCCTGTCTCCACAACACACGTCCTCTCGTCTGCGG
TAGCAGGGACGATGGTGGTGGACGGCGGTGGGTTACAGCGTAAAACGGTAACCAGCATCCTGATGGCGTGGGTGTTTACT
TTACCGGCGGCAATTTTTCTTTCTGGTGGGCTGTACTGGATAGCATTGCAGTTGATTTAA

Upstream 100 bases:

>100_bases
ACTCTTTTTATCGTTAAAAAATGATATTTCACTTTGCCCATGCCGTTAAAGTCACTGATAATGCGTCCGTTCGTAAATTC
AAAATGGCGTAATCTAATAT

Downstream 100 bases:

>100_bases
CCTTCCTGAAAATGCCCGGTCCTGGCGATCGGGCATTTCCATTTTTGACTAGTGATAACCACGCGCGGTCATAAAATCCG
TAATCGCTTTTTCTACATCA

Product: low-affinity inorganic phosphate transporter 2

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKT
MLPGNMESYEPLTVSQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMI
GWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAIFLSGGLYWIALQLI

Sequences:

>Translated_499_residues
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKT
MLPGNMESYEPLTVSQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMI
GWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAIFLSGGLYWIALQLI
>Mature_499_residues
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKT
MLPGNMESYEPLTVSQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMI
GWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAIFLSGGLYWIALQLI

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789360, Length=499, Percent_Identity=99.5991983967936, Blast_Score=1007, Evalue=0.0,
Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=80.7615230460922, Blast_Score=796, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53854; Mature: 53854

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFF
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
SHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
HCCCCHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGTQ
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCE
VTEFHCHPANTFDAIARVKTMLPGNMESYEPLTVSQRSQLRRIMLCISDTSAKLAKLPGV
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
SKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAMTIGEKIGKRGMTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGLQRKTVTSILMAWVFT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHH
LPAAIFLSGGLYWIALQLI
HHHHHHHHCCHHHHEEECC
>Mature Secondary Structure
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAFF
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
SHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
HCCCCHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGTQ
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCE
VTEFHCHPANTFDAIARVKTMLPGNMESYEPLTVSQRSQLRRIMLCISDTSAKLAKLPGV
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
SKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAMTIGEKIGKRGMTY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGLQRKTVTSILMAWVFT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHH
LPAAIFLSGGLYWIALQLI
HHHHHHHHCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 7775463 [H]