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The map label for this gene is nupG

Identifier: 157162426

GI number: 157162426

Start: 3134632

End: 3135888

Strand: Direct

Name: nupG

Synonym: EcHS_A3125

Alternate gene names: 157162426

Gene position: 3134632-3135888 (Clockwise)

Preceding gene: 157162425

Following gene: 157162428

Centisome position: 67.51

GC content: 49.72

Gene sequence:

>1257_bases
ATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTC
CTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTA
TGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCT
ATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATAT
GCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGC
CAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCGAATTAAGCCACATG
CAGCTGTATATTGGCGCAGCACTTTCCGCCATTCTGGTTCTGTTTACCCTGACTCTGCCGCATATTCCGGTTGCTAAACA
GCAAGCGAATCAGAGCTGGACAACCCTGCTGGGCCTCGATGCATTCGCGCTGTTTAAAAACAAGCGTATGGCAATCTTCT
TTATCTTCTCAATGCTGCTGGGCGCGGAACTGCAGATTACCAACATGTTCGGTAATACCTTCCTGCACAGCTTCGACAAA
GATCCGATGTTTGCCAGCAGCTTTATTGTGCAGCATGCGTCAATCATCATGTCGATTTCGCAGATCTCTGAAACCCTGTT
CATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGC
GTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGC
GCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGG
GATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCC
AAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCG
ATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAA

Upstream 100 bases:

>100_bases
ACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCAT
CACGATGTGAGGAAATTAAC

Downstream 100 bases:

>100_bases
TTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTC
ACGCCATCCGCATCGGTTTC

Product: nucleoside permease NupG

Products: NA

Alternate protein names: Nucleoside-transport system protein nupG

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGA
ITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDK
DPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGC
AFDFFNISGSVFVEKEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSVVLAFAFMA
MFKYKHVRVPTGTQTVSH

Sequences:

>Translated_418_residues
MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGA
ITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDK
DPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGC
AFDFFNISGSVFVEKEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSVVLAFAFMA
MFKYKHVRVPTGTQTVSH
>Mature_418_residues
MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGA
ITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDK
DPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGC
AFDFFNISGSVFVEKEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSVVLAFAFMA
MFKYKHVRVPTGTQTVSH

Specific function: Transports nucleosides with a high affinity. Driven by a proton motive force

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli xapB and yegT

Homologues:

Organism=Escherichia coli, GI87082192, Length=418, Percent_Identity=100, Blast_Score=843, Evalue=0.0,
Organism=Escherichia coli, GI1788745, Length=418, Percent_Identity=58.3732057416268, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1788415, Length=417, Percent_Identity=30.4556354916067, Blast_Score=191, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUPG_ECOLI (P0AFF4)

Other databases:

- EMBL:   X06174
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_003521.1
- RefSeq:   NP_417439.4
- ProteinModelPortal:   P0AFF4
- STRING:   P0AFF4
- EnsemblBacteria:   EBESCT00000002361
- EnsemblBacteria:   EBESCT00000002362
- EnsemblBacteria:   EBESCT00000002363
- EnsemblBacteria:   EBESCT00000002364
- EnsemblBacteria:   EBESCT00000016941
- GeneID:   946282
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2932
- KEGG:   eco:b2964
- EchoBASE:   EB0658
- EcoGene:   EG10664
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008944
- HOGENOM:   HBG347023
- OMA:   GIKRVML
- ProtClustDB:   CLSK880543
- BioCyc:   EcoCyc:NUPG-MONOMER
- Genevestigator:   P0AFF4
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR004740
- TIGRFAMs:   TIGR00889

Pfam domain/function: SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 46390; Mature: 46390

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1749b6b8)-; HASH(0x17479ca4)-; HASH(0x11f2fd28)-; HASH(0x1669df9c)-; HASH(0x17138c68)-; HASH(0x16fcbac8)-; HASH(0x1552cb50)-; HASH(0x155142a0)-; HASH(0x16fcba38)-; HASH(0x1746f70c)-; HASH(0x173896fc)-; HASH(0x1693638c)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGI
CCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHMQLYIGAALSAILVLFTLTLP
HHCCCCCEEECCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDK
CCCCCHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCC
DPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHC
GDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKEVSPAIRASAQGMFLMMTNGFGCIL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCEEEEEECCCHHHH
GGIVSGKVVEMYTQNGITDWQTVWLIFAGYSVVLAFAFMAMFKYKHVRVPTGTQTVSH
HHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCH
>Mature Secondary Structure
MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGI
CCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHMQLYIGAALSAILVLFTLTLP
HHCCCCCEEECCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDK
CCCCCHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCC
DPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHC
GDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKEVSPAIRASAQGMFLMMTNGFGCIL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCEEEEEECCCHHHH
GGIVSGKVVEMYTQNGITDWQTVWLIFAGYSVVLAFAFMAMFKYKHVRVPTGTQTVSH
HHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3311747; 9278503