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The map label for this gene is mutY [H]

Identifier: 157162423

GI number: 157162423

Start: 3131901

End: 3132983

Strand: Direct

Name: mutY [H]

Synonym: EcHS_A3122

Alternate gene names: 157162423

Gene position: 3131901-3132983 (Clockwise)

Preceding gene: 157162416

Following gene: 157162425

Centisome position: 67.45

GC content: 55.31

Gene sequence:

>1083_bases
TTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATA
CGGGCGAAAAACGCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTC
AGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTC
GACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAGTGGC
GACCTTACACGGCGGTAAATTCCCGGAAACCTTTGAAGAAGTCGCGGCGTTACCGGGCGTCGGGCGTTCCACCGCAGGCG
CGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCT
GTAAGCGGCTGGCCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGT
GGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGCTCTGTCCGC
TACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAG
CGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGG
TTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACC
TGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCA
TTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTGGCGGCTCCCGT
GGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAG

Upstream 100 bases:

>100_bases
AAAACGGGCATTATCCAAAGTTAGTTGCCGGATGCAAGCATGATAAGGCCGTGGCTGCGGAAAGTTCCGGTTTACACCCT
GCCGTCGCTGTGCTGCAATC

Downstream 100 bases:

>100_bases
CGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGA
TTTTCAGCTGTACCCCGGCG

Product: adenine DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPPTTVNSVTMQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQVATVIPYFERFMARFPTVTDLANAPL
DEVLHLWTGLGYYARARNLHKAAQQVATLHGGKFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYA
VSGWPGKKEVENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWALYPGKKPKQTLPE
RTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQRQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSS
FTGCMDEGNALWYNLAQPPSVGLAAPVERLLQQLRTGAPV

Sequences:

>Translated_360_residues
MPPTTVNSVTMQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQVATVIPYFERFMARFPTVTDLANAPL
DEVLHLWTGLGYYARARNLHKAAQQVATLHGGKFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYA
VSGWPGKKEVENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWALYPGKKPKQTLPE
RTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQRQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSS
FTGCMDEGNALWYNLAQPPSVGLAAPVERLLQQLRTGAPV
>Mature_359_residues
PPTTVNSVTMQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQVATVIPYFERFMARFPTVTDLANAPLD
EVLHLWTGLGYYARARNLHKAAQQVATLHGGKFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAV
SGWPGKKEVENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWALYPGKKPKQTLPER
TGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQRQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSF
TGCMDEGNALWYNLAQPPSVGLAAPVERLLQQLRTGAPV

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI6912520, Length=361, Percent_Identity=36.2880886426593, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI115298648, Length=361, Percent_Identity=36.2880886426593, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI190358497, Length=361, Percent_Identity=36.2880886426593, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI115298654, Length=361, Percent_Identity=36.01108033241, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI115298652, Length=361, Percent_Identity=36.01108033241, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI115298650, Length=361, Percent_Identity=36.01108033241, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1789331, Length=350, Percent_Identity=100, Blast_Score=728, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 40178; Mature: 40046

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTTVNSVTMQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQVATVIP
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
YFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGGKFPETFEE
HHHHHHHHCCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEVENKLWSLSEQ
HHHCCCCCCCHHHHHEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWALYPGKKPKQTLPE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCHHHCCC
RTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQRQIAADNLTQLTAF
CCCEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLAAPVERLLQQLRTGAPV
HHHHHHHEEEEEEHHCCHHHHHCHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PPTTVNSVTMQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQVATVIP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
YFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGGKFPETFEE
HHHHHHHHCCCHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEVENKLWSLSEQ
HHHCCCCCCCHHHHHEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWALYPGKKPKQTLPE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCHHHCCC
RTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQRQIAADNLTQLTAF
CCCEEEEEECCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLAAPVERLLQQLRTGAPV
HHHHHHHEEEEEEHHCCHHHHHCHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]