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The map label for this gene is glpX2

Identifier: 157162389

GI number: 157162389

Start: 3102728

End: 3103693

Strand: Reverse

Name: glpX2

Synonym: EcHS_A3088

Alternate gene names: 157162389

Gene position: 3103693-3102728 (Counterclockwise)

Preceding gene: 157162390

Following gene: 157162388

Centisome position: 66.84

GC content: 56.63

Gene sequence:

>966_bases
ATGATGTCCCTGGCGTGGCCATTATTTCGCGTTACGGAACAGGCAGCGTTGGCTGCCTGGCCGCAAACCGGATGTGGCGA
CAAAAATAAAATTGATGGCCTGGCGGTCACCGCGATGCGCCAGGCATTAAACGACGTCGCTTTTCGTGGACGAGTGGTTA
TCGGCGAAGGAGAAATTGACCATGCGCCGATGCTGTGGATTGGCGAAGAGGTGGGCAAAGGCGATGGGCCAGAAGTCGAT
ATCGCGGTTGATCCCATTGAAGGCACGCGGATGGTGGCGATGGGGCAGAGCAATGCGCTGGCGGTCATGGCTTTCGCCCC
TCGAGATAGCCTGTTACATGCGCCCGATATGTATATGAAAAAGCTGGTTGTTAATCGGCTGGCGGCGGGCGCGATTGATC
TGTCTTTGCCTCTGACGGACAACCTGCGCAACGTGGCGAAAGCGTTAGGCAAACCGCTGGATAAGCTGCGCATGGTTACG
CTGGATAAACCGCGCCTGAGTGCCGCGATTGAGGAAGCAACACAGCTGGGCGTGAAGGTTTTTGCCCTGCCGGATGGCGA
TGTCGCCGCCAGCGTGCTGACCTGCTGGCAGGATAATCCTTACGATGTGATGTACACCATCGGCGGTGCGCCAGAAGGCG
TGATTTCTGCCTGTGCCGTTAAAGCATTAGGCGGGGATATGCAGGCCGAGCTGATTGACTTTTGCCAGGCTAAAGGGGAT
TACACGGAAAATCGGCAGATTGCAGAGCAGGAGCGCAAGCGTTGCAAGGCAATGGGCGTTGACGTTAACCGTGTGTACTC
GCTCGATGAACTGGTGAGGGGGAACGATATCCTCTTTAGCGCTACGGGCGTGACGGGCGGTGAGCTGGTGAACGGTATCC
AACAGACGGCGAATGGGGTGCGGACGCAGACATTACTGATCGGCGGCGCGGACCAAACGTGTAATATAATAGACTCCCTG
CATTGA

Upstream 100 bases:

>100_bases
AGTTGGCGCGGCTGGTTGAGGAAACGCACGGCATCTGGAATGAACAGGCCGAGAAATATCTGCTGGCACAATTTGGCGTT
GATATCGGGGAGGCCGCGCA

Downstream 100 bases:

>100_bases
TGGTGATCTATCGGAAGACCAATGGCGACGCTGACAGAAGATGATGTGCTTGAGCAACTGGATGCACAGGACAATTTATT
TTCATTTATGAAAACTGCGC

Product: fructose-1,6-bisphosphatase II-like protein

Products: NA

Alternate protein names: FBPase 2 class 2; D-fructose-1,6-bisphosphate 1-phosphohydrolase 2 class 2

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALNDVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVD
IAVDPIEGTRMVAMGQSNALAVMAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISACAVKALGGDMQAELIDFCQAKGD
YTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGNDILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSL
H

Sequences:

>Translated_321_residues
MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALNDVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVD
IAVDPIEGTRMVAMGQSNALAVMAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISACAVKALGGDMQAELIDFCQAKGD
YTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGNDILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSL
H
>Mature_321_residues
MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALNDVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVD
IAVDPIEGTRMVAMGQSNALAVMAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISACAVKALGGDMQAELIDFCQAKGD
YTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGNDILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSL
H

Specific function: Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate. Also displays a low activity toward glucose 1,6-bisphosphate, and no activity against ribulose 1,5- bisphosphate, fructose 2,6-bisphosphate, or fructose 1-phosphate

COG id: COG1494

COG function: function code G; Fructose-1,6-bisphosphatase/sedoheptulose 1,7-bisphosphatase and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FBPase class 2 family

Homologues:

Organism=Escherichia coli, GI1789298, Length=321, Percent_Identity=100, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1790360, Length=319, Percent_Identity=57.9937304075235, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPX2_ECOLI (P21437)

Other databases:

- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X14436
- PIR:   A65078
- RefSeq:   AP_003488.1
- RefSeq:   NP_417405.1
- ProteinModelPortal:   P21437
- SMR:   P21437
- STRING:   P21437
- EnsemblBacteria:   EBESCT00000001112
- EnsemblBacteria:   EBESCT00000018408
- GeneID:   947410
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2897
- KEGG:   eco:b2930
- EchoBASE:   EB1226
- EcoGene:   EG11245
- eggNOG:   COG1494
- GeneTree:   EBGT00050000011384
- HOGENOM:   HBG284837
- OMA:   IQRVANG
- ProtClustDB:   PRK12388
- BioCyc:   EcoCyc:EG11245-MONOMER
- BioCyc:   MetaCyc:EG11245-MONOMER
- Genevestigator:   P21437
- InterPro:   IPR004464
- PIRSF:   PIRSF004532
- TIGRFAMs:   TIGR00330

Pfam domain/function: PF03320 FBPase_glpX

EC number: =3.1.3.11

Molecular weight: Translated: 34324; Mature: 34324

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: BINDING 118-118 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALNDVAFRGRVVIGEGEID
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCC
HAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAVMAFAPRDSLLHAPDMYMK
CCCEEEECHHHCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHH
KLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVTLDKPRLSAAIEEATQLGVKV
HHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCHHHHEEEEECCHHHHHHHHHHHHCCEEE
FALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISACAVKALGGDMQAELIDFCQAKGD
EEECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
YTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGNDILFSATGVTGGELVNGIQQTANGV
CCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCC
RTQTLLIGGADQTCNIIDSLH
EEEEEEEECCCCHHHHHHCCC
>Mature Secondary Structure
MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALNDVAFRGRVVIGEGEID
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCC
HAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAVMAFAPRDSLLHAPDMYMK
CCCEEEECHHHCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHH
KLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVTLDKPRLSAAIEEATQLGVKV
HHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCHHHHEEEEECCHHHHHHHHHHHHCCEEE
FALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISACAVKALGGDMQAELIDFCQAKGD
EEECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
YTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGNDILFSATGVTGGELVNGIQQTANGV
CCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCC
RTQTLLIGGADQTCNIIDSLH
EEEEEEEECCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 2546007