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The map label for this gene is yqfA

Identifier: 157162359

GI number: 157162359

Start: 3070002

End: 3070661

Strand: Reverse

Name: yqfA

Synonym: EcHS_A3058

Alternate gene names: 157162359

Gene position: 3070661-3070002 (Counterclockwise)

Preceding gene: 157162360

Following gene: 157162356

Centisome position: 66.13

GC content: 52.12

Gene sequence:

>660_bases
ATGGTTCAGAAGCCCCTCATTAAGCAGGGATATTCGCTGGCAGAGGAAATTGCCAACAGCGTCAGTCACGGCATTGGGTT
GGTGTTTGGTATCGTTGGGCTGGTGTTGCTACTGGTTCAGGCGGTGGATCTCAATGCCAGCGCCACAGCGATAACCAGTT
ACAGCCTCTATGGCGGCAGTATGATCCTGCTGTTCCTCGCTTCGACGCTCTATCACGCCATTCCTCATCAACGGGCAAAA
ATGTGGCTGAAGAAATTTGACCATTGCGCTATTTACCTGTTGATTGCCGGAACCTACACGCCGTTTTTGCTGGTGGGGCT
GGATTCTCCGTTAGCGCGCGGGTTGATGATTGTTATCTGGAGCCTGGCATTGCTGGGTATTCTGTTTAAACTGACCATCG
CGCACCGATTCAAAATTTTATCTCTGGTGACCTATCTGGCGATGGGCTGGCTGTCGCTGGTGGTAATTTATGAAATGGCA
GTTAAGCTCGCGGCGGGCAGCGTTACCTTACTGGCGGTAGGCGGCGTGGTTTATTCGCTCGGGGTGATTTTCTACGTCTG
CAAACGCATTCCATACAACCATGCCATCTGGCACGGCTTCGTGCTCGGCGGTAGTGTGTGCCACTTTCTGGCGATCTATT
TGTATATTGGGCAGGCGTAA

Upstream 100 bases:

>100_bases
TCAAATCAGAATTTATTTTAGCTAACAGGTGTTCACTGGAACTATTCTCAGTTACGCTGGAGAGGTATACATGTGCGTGT
ACGTTTCTCCGGAGTAAGTT

Downstream 100 bases:

>100_bases
TGGGTATGCCTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCGGAGCACAACTGCCGGATTCGGCGTGAA
CGCCTTATCCGATCTACGGG

Product: hemolysin III family channel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDLNASATAITSYSLYGGSMILLFLASTLYHAIPHQRAK
MWLKKFDHCAIYLLIAGTYTPFLLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQA

Sequences:

>Translated_219_residues
MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDLNASATAITSYSLYGGSMILLFLASTLYHAIPHQRAK
MWLKKFDHCAIYLLIAGTYTPFLLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQA
>Mature_219_residues
MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDLNASATAITSYSLYGGSMILLFLASTLYHAIPHQRAK
MWLKKFDHCAIYLLIAGTYTPFLLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQA

Specific function: Unknown

COG id: COG1272

COG function: function code R; Predicted membrane protein, hemolysin III homolog

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0073 (Hly-III) family

Homologues:

Organism=Homo sapiens, GI52630445, Length=213, Percent_Identity=31.4553990610329, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI154759275, Length=208, Percent_Identity=30.7692307692308, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI154759277, Length=232, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1789266, Length=219, Percent_Identity=100, Blast_Score=430, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71996356, Length=217, Percent_Identity=31.7972350230415, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640324, Length=234, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQFA_ECO57 (P67155)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91100
- PIR:   G85945
- RefSeq:   NP_289468.1
- RefSeq:   NP_311798.1
- ProteinModelPortal:   P67155
- EnsemblBacteria:   EBESCT00000027191
- EnsemblBacteria:   EBESCT00000055281
- GeneID:   916413
- GeneID:   959462
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4237
- KEGG:   ecs:ECs3771
- GeneTree:   EBGT00050000008318
- HOGENOM:   HBG717325
- OMA:   RFNAWTH
- ProtClustDB:   PRK15087
- BioCyc:   ECOL83334:ECS3771-MONOMER
- GO:   GO:0019835
- InterPro:   IPR004254
- InterPro:   IPR005744
- PANTHER:   PTHR20855
- PANTHER:   PTHR20855:SF3
- TIGRFAMs:   TIGR01065

Pfam domain/function: PF03006 HlyIII

EC number: NA

Molecular weight: Translated: 23847; Mature: 23847

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17359a08)-; HASH(0x1725e7e4)-; HASH(0x172409c8)-; HASH(0x16fa61d0)-; HASH(0x16fe445c)-; HASH(0x16fd3b1c)-; HASH(0x17086658)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDLNASATAITSYSLYGGS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPFLLVGLDSPLARGLMIVIW
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
SLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMAVKLAAGSVTLLAVGGVVYSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDLNASATAITSYSLYGGS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPFLLVGLDSPLARGLMIVIW
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
SLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMAVKLAAGSVTLLAVGGVVYSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796