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The map label for this gene is grcA [H]

Identifier: 157162060

GI number: 157162060

Start: 2755681

End: 2756064

Strand: Reverse

Name: grcA [H]

Synonym: EcHS_A2736

Alternate gene names: 157162060

Gene position: 2756064-2755681 (Counterclockwise)

Preceding gene: 157162068

Following gene: 157162058

Centisome position: 59.35

GC content: 47.4

Gene sequence:

>384_bases
ATGATTACAGGTATCCAGATTACTAAAGCCGCTAACGACGATCTGCTGAACTCTTTCTGGCTGCTGGACAGCGAAAAAGG
CGAAGCACGTTGCATCGTTGCAAAAGCAGGTTATGCAGAAGATGAAGTGGTTGCAGTAAGCAAACTGGGTGACATTGAAT
ACCGTGAAGTTCCAGTAGAAGTGAAACCAGAAGTTCGCGTTGAAGGTGGTCAACACCTGAACGTTAACGTTCTGCGTCGT
GAAACTCTGGAAGATGCAGTTAAGCATCCGGAAAAATATCCGCAGCTGACCATCCGTGTATCCGGTTATGCAGTTCGCTT
TAACTCTCTGACTCCGGAACAGCAGCGCGACGTTATCGCTCGTACCTTTACTGAAAGCCTGTAA

Upstream 100 bases:

>100_bases
AGGGTGGTTGAGGAGTATATATACACTCAAGCAACAATGGTTTTACCAATTGGCCGCGACAGGCTGAACAAATCAAATAA
TTTTGCCGGGGAGGCATCAC

Downstream 100 bases:

>100_bases
GGCTTAAGTAGCAAGTTACTAAGCGGAAGAGGGCGACCTCTTCCGCTTTTTTTCGTTAATAGAAAATGCGTACCGCGCAA
TAGACCAGCAACAGGGCAAG

Product: autonomous glycyl radical cofactor GrcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVVAVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRR
ETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIARTFTESL

Sequences:

>Translated_127_residues
MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVVAVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRR
ETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIARTFTESL
>Mature_127_residues
MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVVAVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRR
ETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIARTFTESL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins [H]

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain [H]

Homologues:

Organism=Escherichia coli, GI1788933, Length=127, Percent_Identity=100, Blast_Score=261, Evalue=1e-71,
Organism=Escherichia coli, GI1787131, Length=64, Percent_Identity=76.5625, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI48994926, Length=64, Percent_Identity=76.5625, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI1787044, Length=56, Percent_Identity=50, Blast_Score=66, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140 [H]

Pfam domain/function: PF01228 Gly_radical [H]

EC number: NA

Molecular weight: Translated: 14284; Mature: 14284

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVVAVSKLGDIEYREVPVE
CCCCEEEEECCCCCHHHEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCEEEECCEE
VKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIA
ECCCEEECCCCEEEEEEHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHH
RTFTESL
HHHHHCC
>Mature Secondary Structure
MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVVAVSKLGDIEYREVPVE
CCCCEEEEECCCCCHHHEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCEEEECCEE
VKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIA
ECCCEEECCCCEEEEEEHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHH
RTFTESL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA