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The map label for this gene is purC [H]

Identifier: 157161937

GI number: 157161937

Start: 2618812

End: 2619525

Strand: Reverse

Name: purC [H]

Synonym: EcHS_A2607

Alternate gene names: 157161937

Gene position: 2619525-2618812 (Counterclockwise)

Preceding gene: 157161938

Following gene: 157161936

Centisome position: 56.41

GC content: 52.52

Gene sequence:

>714_bases
ATGCAAAAGCAAGCTGAGTTGTATCGTGGTAAAGCGAAAACCGTATACAGCACGGAAAACCCGGACCTGTTGGTGCTCGA
ATTCCGCAATGATACGTCAGCAGGGGATGGCGCGCGCATTGAGCAGTTTGATCGCAAAGGTATGGTGAACAACAAGTTCA
ACTACTTCATTATGAGCAAACTGGCTGAAGCGGGTATCCCGACTCAAATGGAGCGTCTGCTCTCCGATACCGAATGTCTG
GTGAAAAAGCTGGATATGGTGCCGGTTGAGTGTGTCGTGCGTAACCGTGCTGCTGGCTCTCTGGTGAAACGTCTTGGAAT
CGAAGAAGGTATTGAGCTGAACCCGCCGCTGTTCGATCTGTTCCTGAAAAACGACGCCATGCACGATCCGATGGTCAACG
AATCTTACTGCGAAACCTTTGGCTGGGTGAGCAAAGAGAACCTGGCGCGTATGAAAGAGCTGACCTACAAAGCGAACGAC
GTGCTGAAAAAACTGTTCGATGATGCTGGTCTGATTCTGGTCGACTTCAAGCTGGAATTTGGTCTGTACAAAGGCGAAGT
GGTACTGGGTGATGAGTTCTCCCCGGACGGCAGCCGCCTGTGGGACAAAGAAACGCTGGAGAAAATGGACAAAGACCGTT
TCCGCCAGAGCCTCGGTGGCCTGATCGAGGCCTATGAAGCCGTCGCCCGCCGCCTGGGTGTACAGCTGGACTGA

Upstream 100 bases:

>100_bases
GTAGTTCGGATAAGGCGTTCACGCCGCATCCGACAAAACATCCGGCACACCAGACAGCAAAAGATTTTAAAACGTTAATT
CACACCCAGGAGTGATAAAG

Downstream 100 bases:

>100_bases
TTTTTCTGTTCATCATCTTGCCGTGCTGCTGGCACGGCAAGACAACCATTCTCGTAAGATGTGCATTGAACGCTCATTCC
TCCGCCATTTCATCCCGCTT

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFDRKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECL
VKKLDMVPVECVVRNRAAGSLVKRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSLGGLIEAYEAVARRLGVQLD

Sequences:

>Translated_237_residues
MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFDRKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECL
VKKLDMVPVECVVRNRAAGSLVKRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSLGGLIEAYEAVARRLGVQLD
>Mature_237_residues
MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFDRKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECL
VKKLDMVPVECVVRNRAAGSLVKRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSLGGLIEAYEAVARRLGVQLD

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=221, Percent_Identity=32.1266968325792, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI5453539, Length=221, Percent_Identity=32.1266968325792, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI119220559, Length=228, Percent_Identity=30.7017543859649, Blast_Score=101, Evalue=5e-22,
Organism=Escherichia coli, GI1788820, Length=237, Percent_Identity=100, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17531275, Length=219, Percent_Identity=27.8538812785388, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI18860083, Length=222, Percent_Identity=30.6306306306306, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24583917, Length=204, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=9e-16,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 26995; Mature: 26995

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFDRKGMVNNKFNYFIMSK
CCCHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLVKRLGIEEGIELNPPLFDL
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHH
FLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKANDVLKKLFDDAGLILVDFKLEF
HCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
GLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSLGGLIEAYEAVARRLGVQLD
CEEECEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFDRKGMVNNKFNYFIMSK
CCCHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLVKRLGIEEGIELNPPLFDL
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHH
FLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKANDVLKKLFDDAGLILVDFKLEF
HCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
GLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSLGGLIEAYEAVARRLGVQLD
CEEECEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA