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The map label for this gene is ypfJ

Identifier: 157161936

GI number: 157161936

Start: 2617781

End: 2618644

Strand: Reverse

Name: ypfJ

Synonym: EcHS_A2606

Alternate gene names: 157161936

Gene position: 2618644-2617781 (Counterclockwise)

Preceding gene: 157161937

Following gene: 157161935

Centisome position: 56.39

GC content: 53.94

Gene sequence:

>864_bases
ATGCGTTGGCAAGGGCGACGTGAAAGTGACAATGTTGAAGACAGGCGCAACAGCTCTGGTGGTCCGTCTATGGGCGGTCC
CGGTTTTCGCCTGCCGAGCGGTAAAGGCGGGCTGATTTTACTGATAGTCGTGCTGGTTGCAGGCTACTATGGCGTTGATT
TAACCGGGTTGATGACCGGACAGCCGGTTTCCCAACAGCAATCAACGCGGTCAATTAGCCCAAATGAAGACGAAGCCGCA
AAATTCACCTCGGTGATTCTGGCAACTACGGAAGACACCTGGGGACAGCAGTTCGAGAAGATGGGTAAAACCTATCAGCA
ACCGAAGCTGGTCATGTACCGTGGAATGACGCGTACCGGCTGCGGGGCGGGCCAGTCCATAATGGGGCCATTCTATTGCC
CGGCGGATGGCACGGTTTATATCGATCTCTCCTTCTATGATGACATGAAAGACAAACTTGGCGCGGATGGCGATTTTGCC
CAGGGGTACGTTATCGCCCATGAAGTCGGTCATCATGTGCAGAAACTGTTAGGCATCGAGCCGAAAGTTCGTCAACTGCA
ACAAAACGCGACGCAGGCGGAAGTGAACCGCTTATCTGTGCGTATGGAGCTCCAGGCCGACTGTTTTGCCGGTGTCTGGG
GGCATAGTATGCAGCAGCAAGGCGTTCTGGAAACCGGCGATCTGGAAGAGGCGCTGAACGCGGCGCAGGCCATCGGCGAT
GACCGTTTACAACAGCAAAGTCAGGGGCGAGTAGTACCAGACAGTTTCACTCATGGCACTTCTCAGCAACGCTACAGCTG
GTTTAAACGTGGTTTCGACAGCGGCGATCCGGCACAATGCAATACTTTTGGTAAAAGCATTTAA

Upstream 100 bases:

>100_bases
GAACGCTCATTCCTCCGCCATTTCATCCCGCTTTCCTGTGGTAATCTTCCTCTGAACCACCTACGATCATTTCTATAATG
AATATATTGTTGAGGTATCT

Downstream 100 bases:

>100_bases
CTTCCGGGGCAGGGATGGCTGAACTGACTGCGCTTCACACATTAACAGCGCAAATGAAACGTGAAGGGATCCGCCGCTTG
CTGGTGTTGAGCGGGGAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVVLVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAA
KFTSVILATTEDTWGQQFEKMGKTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQQQGVLETGDLEEALNAAQAIGD
DRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCNTFGKSI

Sequences:

>Translated_287_residues
MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVVLVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAA
KFTSVILATTEDTWGQQFEKMGKTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQQQGVLETGDLEEALNAAQAIGD
DRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCNTFGKSI
>Mature_287_residues
MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVVLVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAA
KFTSVILATTEDTWGQQFEKMGKTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQQQGVLETGDLEEALNAAQAIGD
DRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCNTFGKSI

Specific function: Unknown

COG id: COG2321

COG function: function code R; Predicted metalloprotease

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788819, Length=287, Percent_Identity=100, Blast_Score=595, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YPFJ_ECO57 (P64431)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91046
- PIR:   E85890
- RefSeq:   NP_289028.1
- RefSeq:   NP_311364.1
- ProteinModelPortal:   P64431
- EnsemblBacteria:   EBESCT00000026196
- EnsemblBacteria:   EBESCT00000057655
- GeneID:   915256
- GeneID:   957737
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3734
- KEGG:   ecs:ECs3337
- GeneTree:   EBGT00050000011939
- HOGENOM:   HBG321906
- OMA:   QKVYIDL
- ProtClustDB:   CLSK880404
- BioCyc:   ECOL83334:ECS3337-MONOMER
- InterPro:   IPR007343

Pfam domain/function: PF04228 Zn_peptidase

EC number: NA

Molecular weight: Translated: 31461; Mature: 31461

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15a3d4bc)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVVLVAGYYGVDLTGLMTG
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
QPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMGKTYQQPKLVMYRGMTRTG
CCCCHHHHHCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
CGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFAQGYVIAHEVGHHVQKLLGIE
CCCCCCCCCCEECCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQQQGVLETGDLEEALNAAQAIGD
HHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCH
DRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCNTFGKSI
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCCCH
>Mature Secondary Structure
MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVVLVAGYYGVDLTGLMTG
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
QPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMGKTYQQPKLVMYRGMTRTG
CCCCHHHHHCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
CGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFAQGYVIAHEVGHHVQKLLGIE
CCCCCCCCCCEECCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQQQGVLETGDLEEALNAAQAIGD
HHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCH
DRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCNTFGKSI
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796