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The map label for this gene is emrK

Identifier: 157161841

GI number: 157161841

Start: 2513581

End: 2514744

Strand: Reverse

Name: emrK

Synonym: EcHS_A2505

Alternate gene names: 157161841

Gene position: 2514744-2513581 (Counterclockwise)

Preceding gene: 157161844

Following gene: 157161840

Centisome position: 54.16

GC content: 41.84

Gene sequence:

>1164_bases
GTGGAACAGATTAATTCAAATAAAAAACATTCTAACAGAAGAAAATACTTTTCTTTATTGGCGGTAGTTTTATTTATTGC
GTTTTCAGGTGCCTATGCCTATTGGTCAATGGAATTAGAAGACATGATTAGTACAGATGACGCCTATGTCACGGGGAATG
CAGATCCAATTTCTGCACAAGTCTCAGGTAGTGTCACTGTCGTTAATCATAAAGATACGAACTACGTTCGACAAGGTGAC
ATTTTAGTTTCACTGGATAAAACTGATGCCACTATCGCACTCAATAAAGCTAAAAATAATCTGGCAAATATTGTTCGGCA
AACGAATAAACTATACTTACAGGATAAACAATACAGTGCCGAAGTCGCTTCAGCACGTATTCAGTATCAACAATCTTTAG
AAGATTATAACCGTCGAGTGCCGTTAGCGAAGCAGGGGGTTATTTCAAAAGAAACGCTGGAGCATACCAAAGATACGTTA
ATAAGTAGCAAAGCGGCATTGAATGCCGCTATCCAGGCTTATAAAGCGAATAAAGCTTTAGTAATGAACACACCATTAAA
CCGTCAGCCACAAGTCGTTGAAGCGGCGGATGCAACTAAAGAAGCCTGGTTGGCGCTTAAACGTACGGATATTAAGAGTC
CGGTTACCGGCTATATTGCCCAGAGAAGTGTTCAGGTCGGCGAAACAGTGAGCCCCGGACAATCGTTAATGGCTGTCGTA
CCGGCACGTCAAATGTGGGTTAATGCCAACTTTAAAGAAACACAACTCACGGATGTACGGATTGGTCAATCGGTCAATAT
TATCAGCGATCTTTATGGTGAAAATGTTGTGTTTCATGGTCGGGTGACAGGGATCAATATGGGAACCGGCAATGCGTTCT
CCTTATTACCTGCACAAAATGCGACAGGGAACTGGATCAAAATCGTTCAGCGTGTACCGGTTGAAGTTTCTCTTGATCCA
AAAGAACTCATGGAACATCCCTTGCGTATTGGTTTATCGATGACAGCAACTATTGATACGAAGAACGAAGACATTGCCGA
GATGCCTGAGCTGGCTTCAACCGTGACCTCCATGCCGGCTTATACCAGTAAGGCTTTAGTTATCGATACCAGTCCGATAG
AAAAAGAAATTAGCAACATTATTTCGCATAATGGACAACTTTAA

Upstream 100 bases:

>100_bases
CCTGGGATAATGTGCAACACATGCACTGTGTTTGATATGAAGAATGAATGCTCTTTTCATTCAATTCATAAATTTCATCT
ATGAGAAATGAGAGATAATA

Downstream 100 bases:

>100_bases
TGGCAATCACTAAATCAACTCCGGCACCATTAACCGGTGGGACGTTATGGTGCGTCACTATTGCATTGTCATTAGCGACA
TTTATGCAAATGTTGGATTC

Product: drug resistance MFS transporter, membrane fusion protein (MFP) subunit EmrK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL

Sequences:

>Translated_387_residues
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL
>Mature_387_residues
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI1788711, Length=387, Percent_Identity=100, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1789041, Length=378, Percent_Identity=49.4708994708995, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1790519, Length=324, Percent_Identity=27.7777777777778, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=27.9742765273312, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789637, Length=310, Percent_Identity=26.1290322580645, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790024, Length=238, Percent_Identity=22.6890756302521, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EMRK_ECOLI (P52599)

Other databases:

- EMBL:   D78168
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E65010
- RefSeq:   AP_002968.1
- RefSeq:   NP_416869.1
- ProteinModelPortal:   P52599
- SMR:   P52599
- STRING:   P52599
- EnsemblBacteria:   EBESCT00000003825
- EnsemblBacteria:   EBESCT00000017330
- GeneID:   946840
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2365
- KEGG:   eco:b2368
- EchoBASE:   EB3067
- EcoGene:   EG13282
- eggNOG:   COG1566
- GeneTree:   EBGT00050000009283
- HOGENOM:   HBG624965
- OMA:   TATIDTR
- ProtClustDB:   CLSK880372
- BioCyc:   EcoCyc:G7233-MONOMER
- Genevestigator:   P52599
- InterPro:   IPR005694
- InterPro:   IPR006143
- TIGRFAMs:   TIGR00998

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 42586; Mature: 42586

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15c0af24)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCEEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
YTSKALVIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCH
>Mature Secondary Structure
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCEEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
YTSKALVIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503