Click here to switch to the map view.

The map label for this gene is atoC

Identifier: 157161704

GI number: 157161704

Start: 2362848

End: 2364233

Strand: Direct

Name: atoC

Synonym: EcHS_A2361

Alternate gene names: 157161704

Gene position: 2362848-2364233 (Clockwise)

Preceding gene: 157161703

Following gene: 157161705

Centisome position: 50.88

GC content: 48.85

Gene sequence:

>1386_bases
ATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTAAGCACCGCTTTTGCACTACA
AGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGA
TGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATT
CTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTT
TGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACC
AGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTGCAAAGACACCGCC
AAAATTGCCCTTTCTCAGGCCAGCGTCTTGATTAGCGGTGAAAGCGGCACCGGGAAAGAGTTGATTGCCAGAGCGATTCA
CTACAATTCGCGGCGGGCAAAGGGGCCGTTCATTAAAGTCAACTGCGCGGCGCTGCCGGAATCGTTGCTCGAAAGTGAAC
TGTTTGGTCATGAAAAAGGTGCATTTACTGGTGCACAAACCTTGCGTCAGGGATTATTTGAACGAGCCAACGAAGGTACT
CTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGA
ACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATTGCTGCCACCAACCGTGACTTGCAGGCAATGGTAA
AAGAAGGCACCTTCCGTGAAGATCTCTTTTATCGCCTTAACGTTATTCATTTAATACTGCCGCCTCTGCGCGATCGCCGG
GAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCC
GATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCG
TGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTA
AAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGA
ACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAAT
ACGGTATCGATCCGGCGGATGTATAA

Upstream 100 bases:

>100_bases
TTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAAC
CCGCAGGGAAATCAGACTGT

Downstream 100 bases:

>100_bases
CACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCC
AATTTATCCCTTCATATTCA

Product: acetoacetate metabolism regulatory protein AtoC

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLFADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVI
LMTAYAEVETAVEALRCGAFDYVIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHEKGAFTGAQTLRQGLFERANEGT
LLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIKVDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRR
EDISLLANHFLQKFSSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQPVCNAGEV
KTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYKLQEYGIDPADV

Sequences:

>Translated_461_residues
MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLFADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVI
LMTAYAEVETAVEALRCGAFDYVIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHEKGAFTGAQTLRQGLFERANEGT
LLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIKVDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRR
EDISLLANHFLQKFSSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQPVCNAGEV
KTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYKLQEYGIDPADV
>Mature_460_residues
TAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLFADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVIL
MTAYAEVETAVEALRCGAFDYVIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTAK
IALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHEKGAFTGAQTLRQGLFERANEGTL
LLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIKVDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRRE
DISLLANHFLQKFSSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQPVCNAGEVK
TAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYKLQEYGIDPADV

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=100, Blast_Score=949, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=447, Percent_Identity=48.3221476510067, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=42.9487179487179, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788905, Length=455, Percent_Identity=40.2197802197802, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=48.1586402266289, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=46.2732919254658, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1789233, Length=306, Percent_Identity=47.7124183006536, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI87082152, Length=296, Percent_Identity=46.2837837837838, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=43.0817610062893, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786524, Length=338, Percent_Identity=42.603550295858, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1787583, Length=318, Percent_Identity=40.5660377358491, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=38.4615384615385, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87081858, Length=325, Percent_Identity=31.0769230769231, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787487, Length=112, Percent_Identity=37.5, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786784, Length=107, Percent_Identity=33.6448598130841, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786911, Length=121, Percent_Identity=33.8842975206612, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=161, Percent_Identity=26.7080745341615, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789402, Length=107, Percent_Identity=34.5794392523364, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786599, Length=109, Percent_Identity=36.697247706422, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787375, Length=107, Percent_Identity=29.9065420560748, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=126, Percent_Identity=30.952380952381, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=104, Percent_Identity=36.5384615384615, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=212, Percent_Identity=25.9433962264151, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786838, Length=131, Percent_Identity=32.824427480916, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=29.4117647058824, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790552, Length=123, Percent_Identity=28.4552845528455, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATOC_ECOLI (Q06065)

Other databases:

- EMBL:   U17902
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   L13078
- PIR:   B64992
- RefSeq:   AP_002819.1
- RefSeq:   NP_416724.1
- ProteinModelPortal:   Q06065
- SMR:   Q06065
- DIP:   DIP-9190N
- STRING:   Q06065
- EnsemblBacteria:   EBESCT00000003393
- EnsemblBacteria:   EBESCT00000015461
- GeneID:   947444
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2214
- KEGG:   eco:b2220
- EchoBASE:   EB1619
- EcoGene:   EG11668
- eggNOG:   COG2204
- GeneTree:   EBGT00050000008655
- HOGENOM:   HBG740884
- OMA:   REERRII
- ProtClustDB:   PRK11361
- BioCyc:   EcoCyc:ATOC-MONOMER
- Genevestigator:   Q06065
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00448

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat; SSF52172 CheY_like; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 52177; Mature: 52046

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50110 RESPONSE_REGULATORY; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLFADIHPDVVLMDIRMPE
CCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEEECCCCEEEEEECCCC
MDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDYVIKPFDLDELNLIVQRAL
CHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHHH
QLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTAKIALSQASVLISGESGTGKE
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCEEEEECCCCCHHH
LIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHEKGAFTGAQTLRQGLFERANEGT
HHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
LLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIKVDIRIIAATNRDLQAMVKEGTFRE
EEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHHHHHHHCCCHHH
DLFYRLNVIHLILPPLRDRREDISLLANHFLQKFSSENQRDIIDIDPMAMSLLTAWSWPG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCC
NIRELSNVIERAVVMNSGPIIFSEDLPPQIRQPVCNAGEVKTAPVGERNLKEEIKRVEKR
CHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHH
IIMEVLEQQEGNRTRTALMLGISRRALMYKLQEYGIDPADV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLFADIHPDVVLMDIRMPE
CCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEEECCCCEEEEEECCCC
MDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDYVIKPFDLDELNLIVQRAL
CHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHHH
QLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTAKIALSQASVLISGESGTGKE
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCEEEEECCCCCHHH
LIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHEKGAFTGAQTLRQGLFERANEGT
HHHHHHHCCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
LLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIKVDIRIIAATNRDLQAMVKEGTFRE
EEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHHHHHHHCCCHHH
DLFYRLNVIHLILPPLRDRREDISLLANHFLQKFSSENQRDIIDIDPMAMSLLTAWSWPG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCC
NIRELSNVIERAVVMNSGPIIFSEDLPPQIRQPVCNAGEVKTAPVGERNLKEEIKRVEKR
CHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHH
IIMEVLEQQEGNRTRTALMLGISRRALMYKLQEYGIDPADV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225