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The map label for this gene is napF [H]

Identifier: 157161689

GI number: 157161689

Start: 2343982

End: 2344476

Strand: Reverse

Name: napF [H]

Synonym: EcHS_A2346

Alternate gene names: 157161689

Gene position: 2344476-2343982 (Counterclockwise)

Preceding gene: 157161691

Following gene: 157161688

Centisome position: 50.49

GC content: 54.95

Gene sequence:

>495_bases
GTGAAGATTGATGCATCCCGTCGGGGCATACTCACTGGTCGCTGGCGCAAAGCCAGTAACGGTATCCGTCCGCCCTGGTC
CGGTGATGAATCTCATTTTCTGACCCATTGCACCCGCTGCGACGCCTGTATTAATGCTTGTGAAAACAACATTCTGCAAC
GCGGCGCGGGCGGCTATCCGAGCGTTAATTTCAAAAATAACGAGTGCAGCTTCTGTTACGACTGCGCGCAGGCTTGCCCC
GAATCGCTATTTTCTCCGCGCCACACCAGGGCCTGGGATTTGCAGTTCACCATTGGGGACGCGTGCCTGGCGTATCAGTC
TGTTGAATGCCGCCGCTGTCAGGATAGCTGTGAGCCAATGGCAATTATCTTTCGCCCGACGCTCTCCGGGATCTACCAGC
CGCAACTTAATAGCCAACTTTGCAACGGATGTGGAGCATGTGCAGCCAGCTGTCCGGTATCAGCCATAACTGCGGAGTAT
CTCCATGCACACTAA

Upstream 100 bases:

>100_bases
CATCCTTTAATATCTTAGCGGCTATAAAAATGGCTTATTAATTATGCGGTTTATTTGGTCGCTCTCAATTTTCAGAGCGC
GTTAATGATGGAAGGTCAAT

Downstream 100 bases:

>100_bases
CTGGCAAGTTTGCAGCCTGGTCGTGCAGGCCAAAAGCGAACGAATTTCAGACATCAGCACCCAACTGAACGCCTTTCCCG
GCTGTGAAGTTGCTGTCAGC

Product: ferredoxin-type protein

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYPSVNFKNNECSFCYDCAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
LHAH

Sequences:

>Translated_164_residues
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYPSVNFKNNECSFCYDCAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
LHAH
>Mature_164_residues
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYPSVNFKNNECSFCYDCAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
LHAH

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=164, Percent_Identity=99.390243902439, Blast_Score=339, Evalue=6e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18092; Mature: 18092

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
11.0 %Cys+Met (Translated Protein)
9.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
11.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
AIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEYLHAH
EEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
AIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEYLHAH
EEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]