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The map label for this gene is napD

Identifier: 157161688

GI number: 157161688

Start: 2343729

End: 2343992

Strand: Reverse

Name: napD

Synonym: EcHS_A2345

Alternate gene names: 157161688

Gene position: 2343992-2343729 (Counterclockwise)

Preceding gene: 157161689

Following gene: 157161687

Centisome position: 50.48

GC content: 54.55

Gene sequence:

>264_bases
ATGCACACTAACTGGCAAGTTTGCAGCCTGGTCGTGCAGGCCAAAAGCGAACGAATTTCAGACATCAGCACCCAACTGAA
CGCCTTTCCCGGCTGTGAAGTTGCTGTCAGCGACGCGCCGAGCGGTCAGTTGATTGTGGTGGTGGAAGCAGAAGACAGCG
AAACGCTGATCCAAACCATTGAGTCAGTACGCAACGTAGAGGGCGTGCTGGCGGTGTCGCTGGTTTATCACCAGCAGGAA
GAGCAAGGTGAGGAAACACCATGA

Upstream 100 bases:

>100_bases
TCCGGGATCTACCAGCCGCAACTTAATAGCCAACTTTGCAACGGATGTGGAGCATGTGCAGCCAGCTGTCCGGTATCAGC
CATAACTGCGGAGTATCTCC

Downstream 100 bases:

>100_bases
AACTCAGTCGTCGTAGCTTTATGAAAGCTAACGCCGTTGCGGCCGCTGCGGCGGCTGCCGGTCTCAGCGTGCCGGGCGTT
GCCCGCGCCGTTGTTGGTCA

Product: assembly protein for periplasmic nitrate reductase

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 87; Mature: 87

Protein sequence:

>87_residues
MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQE
EQGEETP

Sequences:

>Translated_87_residues
MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQE
EQGEETP
>Mature_87_residues
MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQE
EQGEETP

Specific function: Plays a role in the correct assembly of subunits of the periplasmic napAB enzyme

COG id: COG3062

COG function: function code P; Uncharacterized protein involved in formation of periplasmic nitrate reductase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788535, Length=87, Percent_Identity=100, Blast_Score=175, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPD_ECO57 (P0A9I6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85860
- PIR:   H91015
- RefSeq:   NP_288787.1
- RefSeq:   NP_311123.1
- ProteinModelPortal:   P0A9I6
- SMR:   P0A9I6
- MINT:   MINT-1226944
- EnsemblBacteria:   EBESCT00000028400
- EnsemblBacteria:   EBESCT00000059804
- GeneID:   916802
- GeneID:   957465
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3464
- KEGG:   ecs:ECs3096
- GeneTree:   EBGT00050000010847
- HOGENOM:   HBG466530
- OMA:   AKSERTS
- ProtClustDB:   PRK10553
- BioCyc:   ECOL83334:ECS3096-MONOMER
- GO:   GO:0005737
- InterPro:   IPR005623

Pfam domain/function: PF03927 NapD

EC number: NA

Molecular weight: Translated: 9469; Mature: 9469

Theoretical pI: Translated: 3.85; Mature: 3.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQLIVVVEAEDSETLIQTI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHH
ESVRNVEGVLAVSLVYHQQEEQGEETP
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQLIVVVEAEDSETLIQTI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHH
ESVRNVEGVLAVSLVYHQQEEQGEETP
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796