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The map label for this gene is speG

Identifier: 157161043

GI number: 157161043

Start: 1678319

End: 1678879

Strand: Direct

Name: speG

Synonym: EcHS_A1657

Alternate gene names: 157161043

Gene position: 1678319-1678879 (Clockwise)

Preceding gene: 157161042

Following gene: 157161045

Centisome position: 36.14

GC content: 46.88

Gene sequence:

>561_bases
ATGCCAAGCGCCCACAGTGTTAAGCTACGCCCGCTGGAGCGTGAAGATTTACGCTATGTACATCAACTCGACAATAACGC
CAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTATGATAAGCATATTCACG
ATCAGAGCGAACGGCGCTTTGTGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTT
CATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGC
AATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACA
TTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATT
CGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATA
G

Upstream 100 bases:

>100_bases
GCCTACTGGGAGCCCAATACTTTGCGCTGCCTGGACCGTCGAACTGGCCGCGTTATTACCCCCTAACCTGTTATTGATTT
AAGGAATGTAAGGACACGTT

Downstream 100 bases:

>100_bases
CATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAG
CTGACAGTCCACCGGATTAG

Product: diamine N-acetyltransferase

Products: NA

Alternate protein names: Diamine acetyltransferase; SAT

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHV
HRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
RMCIFQHQYLAEHKTPGQTLLKPTAQ

Sequences:

>Translated_186_residues
MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHV
HRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
RMCIFQHQYLAEHKTPGQTLLKPTAQ
>Mature_185_residues
PSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVH
RRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAIR
MCIFQHQYLAEHKTPGQTLLKPTAQ

Specific function: Regulates polyamine concentration by their degradation. Acts on spermidine, forming N(1)- and N(8)-acetylspermidine

COG id: COG1670

COG function: function code J; Acetyltransferases, including N-acetylases of ribosomal proteins

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Escherichia coli, GI1787867, Length=186, Percent_Identity=100, Blast_Score=389, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATDA_ECO57 (P0A952)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90915
- PIR:   G85763
- RefSeq:   NP_288019.1
- RefSeq:   NP_310317.1
- ProteinModelPortal:   P0A952
- SMR:   P0A952
- EnsemblBacteria:   EBESCT00000027418
- EnsemblBacteria:   EBESCT00000056020
- GeneID:   915270
- GeneID:   961524
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2571
- KEGG:   ecs:ECs2290
- GeneTree:   EBGT00050000010536
- HOGENOM:   HBG742014
- OMA:   LGFMVEG
- ProtClustDB:   PRK15130
- BioCyc:   ECOL83334:ECS2290-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000182
- InterPro:   IPR016181
- Gene3D:   G3DSA:3.40.630.30

Pfam domain/function: PF00583 Acetyltransf_1; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.1.57

Molecular weight: Translated: 21887; Mature: 21756

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51186 GNAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRF
CCCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEE
VVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKL
EEEECCCCCCEEEEEEHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHEEEEE
YLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAIRMCIFQHQYLAEHKTPGQTL
EEEEECCCCCEEEEEEHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LKPTAQ
CCCCCC
>Mature Secondary Structure 
PSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRF
CCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEE
VVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKL
EEEECCCCCCEEEEEEHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHEEEEE
YLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAIRMCIFQHQYLAEHKTPGQTL
EEEEECCCCCEEEEEEHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LKPTAQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796