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The map label for this gene is yneF

Identifier: 157160994

GI number: 157160994

Start: 1627729

End: 1628568

Strand: Reverse

Name: yneF

Synonym: EcHS_A1604

Alternate gene names: 157160994

Gene position: 1628568-1627729 (Counterclockwise)

Preceding gene: 157160995

Following gene: 157160993

Centisome position: 35.07

GC content: 50.24

Gene sequence:

>840_bases
ATGATGCCCGCCATCGCGCTTATTGTGTCGGTTATTGCCTCGGTAGTCATTGGCGGAGCGGGGAGTCTGGCGTTCCCGCT
GCCTGCATTAATCTGGTGTGCAGTGCGCTATACGCCGCAGGTAACATGTCTGTTGACCTTTGTCACCGGTGCGGTGGAAA
TCGTACTGGTGGCAAATTCGGTGATTGATATCTCGGTCGGTTCGCCGTTCTCCATTCCGCAAATGTTCTCCGCACGTCTC
GGTATTGCCACGATGGCGATATGCCCAATTATGGTTTCTTTTAGCGTGGCAGCGATCAATTCGCTAATGAAGCAAGTTGC
GCTGCGAGCCGACTTTGATTTTCTGACTCAGGTTTACTCACGGTCCGGTCTTTATGAGGCGCTGAAAAGTCCATCGCTGA
AACAGACACAACATCTGACTGTCATGCTGCTTGATATCGACTATTTCAAAAGCATTAACGATAACTATGGACATGAATGT
GGCGATAAAGTGTTAAGCGTGTTTGCCCGGCATATTCAGAAGATTGTCGGTGATAAGGGGCTGGTGGCGCGAATGGGCGG
CGAAGAATTTGCTGTTGCAGTGCCGTCGGTGAATCCTGTCGATGGTCTGCTAATGGCGGAAAAAATCCGTAAAGGCGTTG
AACTGCAACCATTCACCTGGCAACAAAAAACGCTTTATCTCACGGTAAGTATTGGCGTCGGTAGTGGTCGCGCATCGTAC
CGAACGCTGACCGATGACTTTAATAAATTGATGGTCGAAGCCGATACGTGTCTGTATCGCTCGAAGAAAGATGGGCGCAA
CCGTACCAGCACTATGCGTTACGGTGAGGAAGTTGTCTGA

Upstream 100 bases:

>100_bases
GGACTGGTTCAGTGAGCAATTCTCAACGGGCGTGTTGATCGTGCCTTGTATGCTGACGTTGGCTATTCCTGGAGTGCTGC
CGCGCTTTAAAGCAGAACAG

Downstream 100 bases:

>100_bases
CATCTTTATTCATTACGCGCGATTTGAATGCAATGTGTTCTACCACTTTATGATCCAGGAAAAGGCGTTTTTCCTCGATT
AAACCATGATCCGCGCCACA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARL
GIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHEC
GDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASY
RTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Sequences:

>Translated_279_residues
MMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARL
GIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHEC
GDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASY
RTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV
>Mature_279_residues
MMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARL
GIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHEC
GDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASY
RTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain

Homologues:

Organism=Escherichia coli, GI1787802, Length=279, Percent_Identity=99.6415770609319, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=37.4269005847953, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787262, Length=212, Percent_Identity=30.188679245283, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=36.2068965517241, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786584, Length=201, Percent_Identity=28.3582089552239, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=193, Percent_Identity=28.4974093264249, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=30.939226519337, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=181, Percent_Identity=30.939226519337, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693134, Length=133, Percent_Identity=32.3308270676692, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=33.1460674157303, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YNEF_ECO57 (Q8XAZ4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A90895
- PIR:   H85722
- RefSeq:   NP_287632.1
- RefSeq:   NP_310156.1
- ProteinModelPortal:   Q8XAZ4
- SMR:   Q8XAZ4
- EnsemblBacteria:   EBESCT00000028988
- EnsemblBacteria:   EBESCT00000055621
- GeneID:   917328
- GeneID:   960644
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2182
- KEGG:   ecs:ECs2129
- GeneTree:   EBGT00050000008781
- HOGENOM:   HBG657465
- OMA:   LAWFSEQ
- ProtClustDB:   CLSK880027
- BioCyc:   ECOL83334:ECS2129-MONOMER
- InterPro:   IPR001054
- InterPro:   IPR000160
- SMART:   SM00267
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00990 GGDEF; SSF55073 A/G_cyclase

EC number: NA

Molecular weight: Translated: 30374; Mature: 30374

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x149bd7ec)-; HASH(0x11ae8cd8)-; HASH(0x139f54fc)-; HASH(0x11a1988c)-;

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECC
VIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RSGLYEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKG
HCCHHHHHCCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
LVARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASY
EEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEECCEEEEEEEEEECCCCCCHH
RTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCH
>Mature Secondary Structure
MMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECC
VIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RSGLYEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKG
HCCHHHHHCCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
LVARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASY
EEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEECCEEEEEEEEEECCCCCCHH
RTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796