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The map label for this gene is ydeM

Identifier: 157160973

GI number: 157160973

Start: 1596456

End: 1597613

Strand: Reverse

Name: ydeM

Synonym: EcHS_A1582

Alternate gene names: 157160973

Gene position: 1597613-1596456 (Counterclockwise)

Preceding gene: 157160974

Following gene: 157160972

Centisome position: 34.41

GC content: 38.69

Gene sequence:

>1158_bases
ATGCACGTTACAGCCAAGCCCTCCAGTTTTCAATGTAATCTCAAATGTGATTACTGTTTTTACCTTGAAAAAGAGTCGCA
GTTTACTCATGAAAAATGGATGGATGACAGCACTCTGAAAGAGTTCATCAAACAATATATCGCAGCGTCTGGCAATCAGG
TCTATTTTACCTGGCAAGGCGGTGAACCCACTCTGGCTGGCCTGGATTTTTTCCGTAAAGTTATTCACTATCAACAACGC
TATGCAGGCCAAAAACGTATTTTTAATGCATTACAAACGAATGGCATTTTATTGAATAATGAATGGTGTGCCTTTCTCAA
AGAACATGAGTTTCTGGTTGGTATCTCGATCGATGGACCCCAGGAGCTACATGACCGTTACAGACGCAGTAATTCAGGTA
ATGGTACTTTTGCAAAAGTGATAGCAGCCATCGAGCGTCTGAAATCATATCAAGTAGAGTTTAATACGTTAACCGTCATT
AATAACGTTAATGTCCATTACCCTCTTGAGGTTTATCATTTTTTAAAATCTATCGGCAGTAAACATATGCAATTTATCGA
ATTGCTAGAAACCGGGACGCCGAATATTGATTTCAGTGGTCATAGTGAGAACACATTCCGTATCATTGATTTTTCTGTGC
CTCCCACGGCTTATGGCAAGTTTATGTCAACCATTTTTATGCAATGGGTTAAAAACGATGTGGGTGAAATTTTCATCCGT
CAGTTTGAAAGCTTTGTCAGCCGTTTTTTGGGGAATGGGCATACCAGTTGTATTTTCCAGGAGTCCTGCAAGGATAATCT
GGTTGTTGAAAGTAATGGAGACATTTACGAATGCGACCATTTTGTCTATCCACAGTACAAAATTGGAAACATTAATAAAT
CTGAACTCAAAACGATGAACAGTGTACAACTGACAGCGCAAAAAAAACGGATTCCAGCGAAATGTCAGCAATGTGCATAT
AAACCTATCTGCAATGGCGGTTGTCCTAAGCATCGTATTACTAAAGTAAACAATGAGACTGTTTCTTATTTTTGCGAAGG
TTATAAAATCCTTTTTTCAACCATGGTACCTTATATGAACGCCATGGTAGAGTTAGCTAAGAACAGAGTACCGCTTTACC
ACATTATGGATGTTGCAAAACAAATGGAGAATAATTAA

Upstream 100 bases:

>100_bases
TCAGGAGAAGTTTAACAATATCAAGAAAGCACTAAGCGAAGCGAAATAACTAAACCTTCATGCGGCGGAATTTTCCGCCG
CCTTATTGAGCGAGATAGCG

Downstream 100 bases:

>100_bases
TAACGCTCTCTTTTAACTCCATTCTTAGCCTCCTTCTTATGGCCTTAACAAGGCACCCGACATAAATTCCTGTCGGGTGT
TTCGTCATCATCATCTTCCC

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME homolog

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQR
YAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRIPAKCQQCAY
KPICNGGCPKHRITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN

Sequences:

>Translated_385_residues
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQR
YAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRIPAKCQQCAY
KPICNGGCPKHRITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN
>Mature_385_residues
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQR
YAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRIPAKCQQCAY
KPICNGGCPKHRITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN

Specific function: Unknown

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family

Homologues:

Organism=Escherichia coli, GI87081923, Length=385, Percent_Identity=100, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI48994973, Length=392, Percent_Identity=41.3265306122449, Blast_Score=334, Evalue=7e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YDEM_ECOLI (P76134)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64903
- RefSeq:   AP_002120.1
- RefSeq:   NP_416014.4
- ProteinModelPortal:   P76134
- IntAct:   P76134
- STRING:   P76134
- EnsemblBacteria:   EBESCT00000004113
- EnsemblBacteria:   EBESCT00000018002
- GeneID:   945981
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1492
- KEGG:   eco:b1497
- EchoBASE:   EB3556
- EcoGene:   EG13795
- eggNOG:   COG0641
- GeneTree:   EBGT00050000010066
- HOGENOM:   HBG484795
- OMA:   MSEIAMH
- ProtClustDB:   CLSK880036
- BioCyc:   EcoCyc:G6787-MONOMER
- Genevestigator:   P76134
- InterPro:   IPR007197

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 44518; Mature: 44518

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQG
CCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEC
GEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGP
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEECCHHHHHHCCCCEEEEEECCCH
QELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVINNVNVHYPLEVYHFLKSIGS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEECCCEECHHHHHHHHHHHHH
KHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKIGNINKSELKTMN
HHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCEEECCCEECCCEEECCCCHHHHHHHH
SVQLTAQKKRIPAKCQQCAYKPICNGGCPKHRITKVNNETVSYFCEGYKILFSTMVPYMN
HHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
AMVELAKNRVPLYHIMDVAKQMENN
HHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQG
CCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEC
GEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGP
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEECCHHHHHHCCCCEEEEEECCCH
QELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVINNVNVHYPLEVYHFLKSIGS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEECCCEECHHHHHHHHHHHHH
KHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKIGNINKSELKTMN
HHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCEEECCCEECCCEEECCCCHHHHHHHH
SVQLTAQKKRIPAKCQQCAYKPICNGGCPKHRITKVNNETVSYFCEGYKILFSTMVPYMN
HHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
AMVELAKNRVPLYHIMDVAKQMENN
HHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503