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The map label for this gene is dosC [H]

Identifier: 157160966

GI number: 157160966

Start: 1582570

End: 1583952

Strand: Reverse

Name: dosC [H]

Synonym: EcHS_A1575

Alternate gene names: 157160966

Gene position: 1583952-1582570 (Counterclockwise)

Preceding gene: 157160967

Following gene: 157160965

Centisome position: 34.11

GC content: 44.76

Gene sequence:

>1383_bases
ATGGAGATGTATTTTAAAAGAATGAAAGATGAGTGGACCGGACTTGTCGAACAGGCAGATCCGCCCATTCGTGCTAAAGC
CGCGGAAATTGCCGTTGCGCATGCTCATTATCTGAGTATTGAGTTTTATCGAATTGTCCGCATCGACCCGCATGCCGAAG
AATTCTTGAGTAATGAACAAGTTGAGCGGCAGTTGAAAAGTGCGATGGAGCGCTGGATTATTAACGTGCTTTCTGCCCAG
GTTGACGATGTCGAAAGGCTAATACAAATCCAGCATACCGTCGCGGAAGTGCATGCCCGCATAGGAATTCCGGTAGAAAT
TGTCGAGATGGGGTTTCGGGTGCTGAAAAAAATCCTCTATCCGGTAATCTTCTCTTCGGATTATTCTGCCGCAGAAAAAC
TTCAGGTCTACCATTTCTCGATTAACAGTATTGATATCGCTATGGAAGTGATGACCCGCGCGTTTACCTTTAGTGACAGT
AGTGCCTCAAAGGAAGATGAAAACTATCGTATCTTCTCGTTACTGGAAAACGCCGAAGAAGAAAAAGAACGGCAAATAGC
CTCAATACTTTCATGGGAAATAGATATTATCTATAAAGTCCTGCTGGATTCTGATTTAGGCAGTAGTTTGCCTTTAAGCC
AGGCTGATTTTGGCCTGTGGTTTAACCATAAAGGTCGACATTATTTTAGCGGCATTGCCGAAGTGGGCCATATCTCCCGT
CTGATTCAGGATTTCGACGGTATTTTCAATCAAACCATGCGTAACACCAGGAATTTGAATAACAGAAGTCTGCGGGTGAA
ATTTTTATTACAGATAAGAAATACCGTATCGCAAATTATTACCTTGCTGCGTGAATTGTTTGAAGAAGTATCGCGCCACG
AAGTCGGTATGGATGTACTGACGAAATTACTTAACCGCCGTTTCCTGCCGACTATCTTCAAACGCGAAATTGCCCATGCC
AACCGGACCGGTACACCGCTGTCAGTGCTGATTATTGACGTTGATAAATTCAAAGAGATCAACGATACGTGGGGCCATAA
CACTGGCGATGAAATTCTGCGTAAAGTCTCTCAGGCCTTTTATGACAACGTCCGCAGTAGTGATTATGTTTTCCGCTACG
GGGGCGATGAATTTATCATTGTATTGACTGAAGCTTCTGAAAACGAAACGTTACGTACCGCAGAACGTATTCGCAGTCGG
GTGGAGAAAACCAAACTGAAAGCCGCAAACGGCGAGGATATTGCCCTCTCACTTTCCATCGGTGCCGCCATGTTTAATGG
TCATCCTGACTATGAGCGCCTCATTCAAATAGCCGATGAAGCTCTGTATATCGCCAAAAGACGAGGTAGAAACCGTGTTG
AACTCTGGAAAGCCAGTCTTTAG

Upstream 100 bases:

>100_bases
ATCTTCATATTTCAGGAGAATATGGTGCAGTTAGTCGATCTCGCACGTTGCGTTTCGTTTTGATTCCGTCTGATTTCCCA
GCCTTATAAGGGTGTGAATT

Downstream 100 bases:

>100_bases
ATGCGCCAGGATGCAGAGGTAATCATGAAGCTAACCGATGCGGAAAATGCCGCCGATGGCATTTTTTTCCCCGCCCTTGA
ACAAAATATGATGGGCGCGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQ
VDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHA
NRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSR
VEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL

Sequences:

>Translated_460_residues
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQ
VDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHA
NRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSR
VEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
>Mature_460_residues
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQ
VDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHA
NRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSR
VEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=460, Percent_Identity=99.7826086956522, Blast_Score=938, Evalue=0.0,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=34.5911949685535, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=36.5269461077844, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=187, Percent_Identity=35.2941176470588, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=31.5789473684211, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=33.7278106508876, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=35.8024691358025, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=33.1210191082803, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788956, Length=153, Percent_Identity=32.6797385620915, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=31.5217391304348, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787802, Length=131, Percent_Identity=33.587786259542, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=29.2817679558011, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53164; Mature: 53164

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHCHHH
VERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
PVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFSLLENAEE
HHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
EKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAF
HHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
YDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSI
HHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHCHHH
VERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
PVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFSLLENAEE
HHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
EKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAF
HHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
YDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSI
HHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]