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The map label for this gene is dos [H]

Identifier: 157160965

GI number: 157160965

Start: 1580146

End: 1582569

Strand: Reverse

Name: dos [H]

Synonym: EcHS_A1574

Alternate gene names: 157160965

Gene position: 1582569-1580146 (Counterclockwise)

Preceding gene: 157160966

Following gene: 157160964

Centisome position: 34.08

GC content: 50.21

Gene sequence:

>2424_bases
ATGCGCCAGGATGCAGAGGTAATCATGAAGCTAACCGATGCGGAAAATGCCGCCGATGGCATTTTTTTCCCCGCCCTTGA
ACAAAATATGATGGGCGCGGTGTTAATTAACGAAAATGATGAAGTGATGTTTTTCAACCCCGCCGCAGAGAAGCTCTGGG
GATACAAACGTGAAGAAGTCATTGGCAATAACATTGATATGCTGATTCCGCGGGATTTGCGTCCTGCGCATCCTGAATAC
ATTCGTCATAACCGTGAAGGCGGTAAAGCGCGTGTTGAGGGGATGAGTCGGGAGCTGCAGCTGGAGAAAAAAGACGGCAG
TAAAATCTGGACCCGTTTTGCGCTATCGAAAGTGAGCGCCGAGGGGAAAGTTTATTACCTGGCGCTGGTACGGGATGCCA
GCGTAGAAATGGCGCAAAAAGAACAGACCCGACAATTGATTATTGCCGTTGACCATCTCGACCGACCGGTGATTGTCCTC
GATCCGGAACGCCATATTGTGCAGTGCAATCGCGCATTTACCGAAATGTTTGGTTACTGCATTAGCGAAGCCAGCGGTAT
GCAGCCCGATACACTCCTGAACATTCCTGAATTCCCTGCCGATAACCGCATTCGTTTACAACAGTTGCTATGGAAAACCG
CCCGCGATCAGGACGAATTTCTGCTGTTGACGCGCACCGGTGAAAAAATCTGGATTAAAGCCTCTATCAGCCCGGTTTAT
GACGTGCTCGCGCATCTGCAGAACCTGGTAATGACTTTCTCGGATATCACCGAAGAACGGCAGATTCGCCAGCTTGAAGG
CAATATTCTCGCCGCCATGTGCAGCAGCCCGCCATTTCATGAAATGGGGGAAATCATTTGTCGTAACATCGAATCTGTAC
TCAACGAATCGCATGTTTCGCTGTTCGCACAGCGCAACGGGATGCCGATACACTGGGCGTCATCTTCCCACGGTGCAGAA
ATTCAAAATGCGCAAAGCTGGTCAGCGACCATTCGTCAGCGTGATGGCGCGCCTGCGGGGATCCTGCAAATTAAAACCTC
GTCAGGAGCAGAAACCAGCGCCTTTATCGAACGCGTGGCAGATATCAGCCAGCATATGGCCGCGCTGGCGCTGGAACAGG
AAAAAAGCCGTCAGCATATTGAACAACTCATCCAATTTGATCCGATGACCGGTCTGCCAAATCGCAATAACCTGCACAAT
TACCTCGATGACCTGGTCGACAAAGCCGTCTCTCCCGTGGTGTATCTCATCGGTGTTGACCATATTCAGGATGTGATTGA
TAGCCTTGGCTATGCGTGGGCCGATCAGGCATTGCTGGAAGTGGTCAATCGCTTTCGTGAAAAACTCAAACCGGATCAGT
ATCTCTGTCGTATCGAAGGTACGCAGTTTGTCCTCGTGAGCCTCGAAAACGACGTCAGTAACATTACCCAAATCGCCGAT
GAGCTACGGAATGTGGTCAGCAAGCCGATAATGATTGACGATAAACCCTTCCCGCTTACCTTGAGTATTGGCATCAGCTA
CGACGTGGGTAAAAACCGCGATTACTTGCTCTCCACTGCTCACAATGCAATGGATTATATTCGCAAGAATGGCGGTAACG
GCTGGCAGTTCTTCAGCCCGGCGATGAACGAAATGGTAAAAGAGCGTTTGGTTTTAGGCGCAGCGCTGAAAGAAGCGATT
AGCAATAACCAACTGAAACTGGTTTACCAGCCGCAAATCTTCGCAGAAACGGGTGAACTGTACGGCATCGAAGCCCTTGC
TCGCTGGCACGATCCCCTGCATGGTCATGTGCCCCCTTCACGGTTTATTCCTCTCGCAGAAGAGATTGGTGAAATCGAAA
ATATTGGGCGCTGGGTCATCGCGGAAGCTTGCCGTCAGTTAGCAGAATGGCGTAGCCAGAATATTCATATCCCGGCGTTA
TCCGTGAACTTGTCGGCGCTGCACTTTCGCAGTAATCAACTGCCTTATCAGGTGTCTGATGCAATGCACGCCTGGGGTAT
TGACGGCCACCAGCTGACGGTAGAAATCACGGAAAGCATGATGATGGAACACGATACCGAAATCTTTAAGCGCATTCAGA
TCCTGCGTGATATGGGCGTGGGCTTATCGGTAGATGATTTTGGTACGGGCTTTTCCGGATTATCCCGCTTAGTCAGTCTT
CCGGTAACGGAAATCAAAATTGACAAAAGTTTTGTCGATCGTTGTCTGACCGAAAAACGCATCCTTGCCTTACTTGAAGC
CATTACCAGCATTGGGCAAAGCCTCAATTTAACCGTCGTGGCGGAAGGCGTCGAAACCAAAGAGCAATTTGAGATGCTAC
GCAAGATCCACTGTCGCGTTATTCAGGGATATTTCTTTTCCCGCCCCCTACCCGCCGAAGAAATTCCAGGCTGGATGAGC
AGCGTGTTACCGCTGAAAATCTGA

Upstream 100 bases:

>100_bases
TCCTGACTATGAGCGCCTCATTCAAATAGCCGATGAAGCTCTGTATATCGCCAAAAGACGAGGTAGAAACCGTGTTGAAC
TCTGGAAAGCCAGTCTTTAG

Downstream 100 bases:

>100_bases
CAAATTCCTCTCGCCCGCACTCGCGGGTTTTCATTTAACGTGACACTGTCACTTAAATACTGTGATTTCAGCTACGATTC
CGGGAGATTCCTTCCTTAAC

Product: cAMP phosphodiesterase

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 807; Mature: 807

Protein sequence:

>807_residues
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEY
IRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
DPERHIVQCNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFAQRNGMPIHWASSSHGAE
IQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAI
SNNQLKLVYQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPAL
SVNLSALHFRSNQLPYQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMS
SVLPLKI

Sequences:

>Translated_807_residues
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEY
IRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
DPERHIVQCNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFAQRNGMPIHWASSSHGAE
IQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAI
SNNQLKLVYQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPAL
SVNLSALHFRSNQLPYQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMS
SVLPLKI
>Mature_807_residues
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEY
IRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
DPERHIVQCNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFAQRNGMPIHWASSSHGAE
IQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAI
SNNQLKLVYQPQIFAETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPAL
SVNLSALHFRSNQLPYQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMS
SVLPLKI

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=799, Percent_Identity=99.4993742177722, Blast_Score=1646, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=439, Percent_Identity=33.9407744874715, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI226510982, Length=413, Percent_Identity=31.2348668280872, Blast_Score=187, Evalue=3e-48,
Organism=Escherichia coli, GI1790496, Length=244, Percent_Identity=36.8852459016393, Blast_Score=148, Evalue=2e-36,
Organism=Escherichia coli, GI87081845, Length=238, Percent_Identity=34.8739495798319, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI87081980, Length=261, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=4e-33,
Organism=Escherichia coli, GI1786507, Length=325, Percent_Identity=29.8461538461538, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=33.8775510204082, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1788502, Length=255, Percent_Identity=29.8039215686275, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787055, Length=275, Percent_Identity=30.5454545454545, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1788849, Length=462, Percent_Identity=25.5411255411255, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87082096, Length=265, Percent_Identity=27.5471698113208, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 91269; Mature: 91269

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEV
CCCCHHHHEEECCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEECCHHHHHCCCCHHHH
IGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSA
CCCCCCEEECCCCCCCCHHHHHCCCCCCHHHHHCCCHHEEEECCCCHHHHHHHHHHHHCC
EGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYC
CCCEEEEEEEECCHHHHHHHHHHHEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
ISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
HHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHH
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHH
LFAQRNGMPIHWASSSHGAEIQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVA
HHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
DISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEHH
HIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
HHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCHHHHHHHHH
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSP
HHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCH
AMNEMVKERLVLGAALKEAISNNQLKLVYQPQIFAETGELYGIEALARWHDPLHGHVPPS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHCCCCCCCCCHH
RFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPYQVSD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEEECCCCCCHHHH
AMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
HHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRV
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
IQGYFFSRPLPAEEIPGWMSSVLPLKI
HHHHHHCCCCCHHHCCCHHHHCCCCCC
>Mature Secondary Structure
MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEV
CCCCHHHHEEECCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEECCHHHHHCCCCHHHH
IGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSA
CCCCCCEEECCCCCCCCHHHHHCCCCCCHHHHHCCCHHEEEECCCCHHHHHHHHHHHHCC
EGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYC
CCCEEEEEEEECCHHHHHHHHHHHEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
ISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVY
HHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHH
DVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHH
LFAQRNGMPIHWASSSHGAEIQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVA
HHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
DISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEHH
HIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIAD
HHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCHHHHHHHHH
ELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSP
HHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEECCH
AMNEMVKERLVLGAALKEAISNNQLKLVYQPQIFAETGELYGIEALARWHDPLHGHVPPS
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHCCCCCCCCCHH
RFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPYQVSD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEEECCCCCCHHHH
AMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSL
HHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
PVTEIKIDKSFVDRCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRV
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
IQGYFFSRPLPAEEIPGWMSSVLPLKI
HHHHHHCCCCCHHHCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]