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The map label for this gene is sfcA [H]

Identifier: 157160955

GI number: 157160955

Start: 1570784

End: 1572481

Strand: Reverse

Name: sfcA [H]

Synonym: EcHS_A1564

Alternate gene names: 157160955

Gene position: 1572481-1570784 (Counterclockwise)

Preceding gene: 157160956

Following gene: 157160954

Centisome position: 33.86

GC content: 51.88

Gene sequence:

>1698_bases
ATGGAACCAAAAACAAAAAAACAGCGTTCGCTTTATATCCCTTACGCTGGCCCTGTACTGCTGGAATTTCCGTTGTTGAA
TAAAGGCAGTGCCTTCAGCATGGAAGAACGCCGTAACTTCAACCTGCTGGGGTTACTGCCGGAAGTGGTCGAAACCATCG
AAGAACAAGCGGAACGAGCATGGATCCAGTATCAGGGATTCAAAACCGAAATCGACAAACACATCTACCTGCGTAACATC
CAGGACACTAACGAAACCCTCTTCTACCGTCTGGTAAACAATCATCTTGATGAGATGATGCCTGTTATTTATACCCCAAC
CGTCGGCGCAGCCTGTGAGCGTTTTTCTGAGATCTACCGCCGTTCACGCGGCGTGTTTATCTCTTACCAGAACCGGCACA
ATATGGACGATATTCTGCAAAACGTGCCGAACCATAATATTAAAGTGATTGTGGTGACTGACGGTGAACGTATTCTGGGG
CTTGGTGACCAGGGCATCGGCGGGATGGGCATTCCGATCGGTAAACTGTCGCTCTATACCGCCTGTGGCGGCATCAGCCC
GGCGTATACCCTTCCGGTGGTGCTGGATGTCGGAACGAACAACCAACAGCTGCTTAACGATCCGCTGTATATGGGCTGGC
GTAATCCGCGTATCACTGACGACGAATACTATGAATTCGTTGATGAATTTATCCAGGCTGTGAAACAACGCTGGCCAGAC
GTGCTGTTGCAGTTTGAAGACTTTGCTCAAAAAAATGCGATGCCGTTACTTAACCGCTATCGCAATGAAATTTGTTCTTT
TAACGATGACATTCAGGGCACTGCGGCGGTAACAGTCGGCACACTGATCGCAGCAAGCCGAGCGGCAGGTGGTCAGTTAA
GCGAGAAAAAAATCGTCTTCCTTGGTGCAGGTTCAGCGGGATGCGGCATTGCCGAAATGATCATCGCCCAGACCCAGCGC
GAAGGATTAAGCGAGGAAGCGGCGCGGCAGAAAGTCTTTATGGTCGATCGCTTTGGCTTGCTGACTGACAAGATGCCGAA
CCTGCTGCCTTTCCAGACCAAACTGGTGCAGAAGCGCGAAAACCTCAGTGACTGGGATACCGACAGCGATGTGCTGTCAC
TGCTGGATGTGGTGCGCAATGTAAAACCAGATATTCTGATTGGCGTCTCAGGACAGACCGGGCTGTTTACGGAAGAGATC
ATCCGTGAGATGCATAAACACTGTCCGCGTCCGATCGTGATGCCGCTGTCTAACCCGACGTCACGCGTGGAAGCCACACC
GCAGGACATTATCGCCTGGACCGAAGGTAACGCGCTGGTCGCCACGGGCAGCCCGTTTAATCCAGTGGTATGGAAAGATA
AAATCTACCCTATCGCCCAGTGTAACAACGCCTTTATTTTCCCGGGCATCGGCCTGGGTGTTATTGCTTCCGGCGCGTCA
CGTATCACCGATGAGATGCTGATGTCGGCAAGTGAAACGCTGGCGCAGTATTCACCATTGGTGCTGAACGGCGAAGGTAT
GGTACTGCCGGAACTGAAAGATATTCAGAAAGTCTCCCGCGCAATTGCGTTTGCGGTTGGCAAAATGGCGCAGCAGCAAG
GCGTGGCGGTGAAAACCTCTGCCGAAGCCCTGCAACAGGCCATTGACGATAATTTCTGGCAAGCCGAATACCGCGACTAC
CGCCGTACCTCCATCTAA

Upstream 100 bases:

>100_bases
TTTGTTTTTATCTGCTTTATACTTGAGGCCGACGCCCTGGCGGTAAAGCAAAGACGATAAAAGCCCCCCAGGGATGGATA
TTCAAAAAAGAGTGAGTGAC

Downstream 100 bases:

>100_bases
GCCTGCGCCCGGTAGTGAAGGCTACCGGGCTATTTCCCTCTCCCTTTTTCAGTTCTCATCCATACTGGGTAGTGGCGAAT
AAATCTCATTTGCCTCACCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNI
QDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQR
EGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEI
IREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
RITDEMLMSASETLAQYSPLVLNGEGMVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDY
RRTSI

Sequences:

>Translated_565_residues
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNI
QDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQR
EGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEI
IREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
RITDEMLMSASETLAQYSPLVLNGEGMVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDY
RRTSI
>Mature_565_residues
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNI
QDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQR
EGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEI
IREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
RITDEMLMSASETLAQYSPLVLNGEGMVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDY
RRTSI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=540, Percent_Identity=42.7777777777778, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI62420882, Length=515, Percent_Identity=44.0776699029126, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI62420880, Length=515, Percent_Identity=44.0776699029126, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI239049447, Length=515, Percent_Identity=44.0776699029126, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI4505145, Length=552, Percent_Identity=40.9420289855072, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI270265879, Length=463, Percent_Identity=44.060475161987, Blast_Score=391, Evalue=1e-108,
Organism=Escherichia coli, GI87081919, Length=565, Percent_Identity=99.8230088495575, Blast_Score=1169, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17537199, Length=567, Percent_Identity=43.9153439153439, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322823, Length=521, Percent_Identity=45.6813819577735, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI21356279, Length=516, Percent_Identity=44.7674418604651, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281362674, Length=516, Percent_Identity=44.7674418604651, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281362672, Length=516, Percent_Identity=44.7674418604651, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24646388, Length=512, Percent_Identity=42.1875, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24646386, Length=512, Percent_Identity=42.1875, Blast_Score=431, Evalue=1e-120,
Organism=Drosophila melanogaster, GI78707242, Length=537, Percent_Identity=38.9199255121043, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI78707238, Length=537, Percent_Identity=38.9199255121043, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281363505, Length=540, Percent_Identity=35.9259259259259, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707236, Length=540, Percent_Identity=35.9259259259259, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707232, Length=507, Percent_Identity=38.2642998027613, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707240, Length=513, Percent_Identity=39.766081871345, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281363503, Length=538, Percent_Identity=35.6877323420074, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI19922384, Length=536, Percent_Identity=31.3432835820896, Blast_Score=275, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 63182; Mature: 63182

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERA
CCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
WIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYR
HHHCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHH
RSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYT
HCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHH
ACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
HHCCCCCCEEEEEEEEECCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCHH
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVF
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRE
EECCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEEHHHHHHHHHCCCCCCCHHHHHHHHHC
NLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPT
CCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCH
SRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
HHCCCCHHHEEEEECCCEEEECCCCCCCCEECCCCCCCEECCCEEEECCCCHHHHHCCHH
RITDEMLMSASETLAQYSPLVLNGEGMVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTS
HHHHHHHHHHHHHHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
AEALQQAIDDNFWQAEYRDYRRTSI
HHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERA
CCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
WIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYR
HHHCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHH
RSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYT
HCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHH
ACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
HHCCCCCCEEEEEEEEECCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCHH
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVF
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRE
EECCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEEHHHHHHHHHCCCCCCCHHHHHHHHHC
NLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPT
CCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCH
SRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
HHCCCCHHHEEEEECCCEEEECCCCCCCCEECCCCCCCEECCCEEEECCCCHHHHHCCHH
RITDEMLMSASETLAQYSPLVLNGEGMVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTS
HHHHHHHHHHHHHHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
AEALQQAIDDNFWQAEYRDYRRTSI
HHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA