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The map label for this gene is mcbR

Identifier: 157160929

GI number: 157160929

Start: 1541232

End: 1541897

Strand: Direct

Name: mcbR

Synonym: EcHS_A1536

Alternate gene names: 157160929

Gene position: 1541232-1541897 (Clockwise)

Preceding gene: 157160928

Following gene: 157160931

Centisome position: 33.19

GC content: 43.69

Gene sequence:

>666_bases
ATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGG
CGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAG
CATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGT
CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCA
AGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATG
TAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTC
AGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATCCAGCGGAATTACGGGAGCATATAGAAAACTATCATCT
GTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTA
TTTTATACCAGCAATACAATCGTTAA

Upstream 100 bases:

>100_bases
AACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAACTCCTTCTGCCATGCAGGCAGGGTTGGACAGA
AAACACGCGAAGGAATTAAA

Downstream 100 bases:

>100_bases
AGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCGCCATGCGATATTCACGCCGACACCATAATTTCG
CCCGGGCGCAGGTTCGTAAT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKR
QLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR

Sequences:

>Translated_221_residues
MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKR
QLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR
>Mature_220_residues
PGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQ
LDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWVR
MGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87081914, Length=221, Percent_Identity=100, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): MCBR_ECOLI (P76114)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64897
- RefSeq:   AP_002073.1
- RefSeq:   NP_415967.2
- ProteinModelPortal:   P76114
- SMR:   P76114
- DIP:   DIP-12749N
- IntAct:   P76114
- MINT:   MINT-1303162
- STRING:   P76114
- EnsemblBacteria:   EBESCT00000004686
- EnsemblBacteria:   EBESCT00000014406
- GeneID:   946014
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1445
- KEGG:   eco:b1450
- EchoBASE:   EB3535
- EcoGene:   EG13773
- eggNOG:   COG1802
- GeneTree:   EBGT00050000008287
- HOGENOM:   HBG700007
- OMA:   ERIENYH
- ProtClustDB:   PRK11414
- BioCyc:   EcoCyc:G6761-MONOMER
- Genevestigator:   P76114
- GO:   GO:0005622
- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00895
- SMART:   SM00345

Pfam domain/function: PF07729 FCD; PF00392 GntR

EC number: NA

Molecular weight: Translated: 25152; Mature: 25021

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVS
CCCCCCEEEEEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQ
CCHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWVRMGPGLHYLYEAINPAELRE
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
HIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQLGMSITPVREALLRLVS
CCCCCEEEEEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVENLTPQDLAELQELLEKLQ
CCHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWVRMGPGLHYLYEAINPAELRE
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
HIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQYNR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503