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The map label for this gene is paaX [H]

Identifier: 157160881

GI number: 157160881

Start: 1486732

End: 1487682

Strand: Direct

Name: paaX [H]

Synonym: EcHS_A1486

Alternate gene names: 157160881

Gene position: 1486732-1487682 (Clockwise)

Preceding gene: 157160880

Following gene: 157160882

Centisome position: 32.02

GC content: 50.58

Gene sequence:

>951_bases
ATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGTA
TGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGCTTGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTG
AGCGTTTCGTGCGCACCGCTTTGTTTCGTCTTAATAAAGAAGGCTGGCTGGATGTTTCCCGCATCGGGCGACGCAGTTTC
TATAGCCTCAGTGATAAAGGCTTGCGCCTGACGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGA
TGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAAGCGACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAG
GTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCGGATGTACAGACACTTTTGCATGAAGCG
GGGGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGA
AGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTGTTAA
AAGAGACGGCAGACGAGTTAACCCCGGAGCGTGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTC
CTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCTCACTGGGCAGGGCATACGGCGCGTCAGCTGTGTATCAACAT
TTATCAGCGCGTAGCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCAGTCGGTGAACTGCCTGCGCCGG
GAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTACCAGATAG

Upstream 100 bases:

>100_bases
CGTGTCGTCTGGCCCTGGTGAGGTAAAGCGTCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACA
ATAACAACAGACTGAATCGA

Downstream 100 bases:

>100_bases
ATGGTCTGACTCCGGTTGTGCCAGAAGAGAGCTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAG
GGCGTTTACGTTGGGCCTAA

Product: phenylacetic acid degradation operon negative regulatory protein PaaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLAALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSF
YSLSDKGLRLTRRAESKIYRAEQPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETADELTPERAFHIQLLLIHFYRRVV
LKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAALAFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR

Sequences:

>Translated_316_residues
MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLAALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSF
YSLSDKGLRLTRRAESKIYRAEQPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETADELTPERAFHIQLLLIHFYRRVV
LKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAALAFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR
>Mature_315_residues
SKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLAALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFY
SLSDKGLRLTRRAESKIYRAEQPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEAG
VADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETADELTPERAFHIQLLLIHFYRRVVL
KDPLLPEELLPAHWAGHTARQLCINIYQRVAPAALAFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR

Specific function: Negative regulator of the paaZ and paaABCDEFGHIJK catabolic operons. Binds the consensus sequence 5'- WWTRTGATTCGYGWT-3'. Binding of paaX is specifically inhibited by phenylacetyl-coenzyme A [H]

COG id: COG3327

COG function: function code K; Phenylacetic acid-responsive transcriptional repressor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787666, Length=316, Percent_Identity=99.0506329113924, Blast_Score=637, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012906
- InterPro:   IPR013225
- InterPro:   IPR011965 [H]

Pfam domain/function: PF07848 PaaX; PF08223 PaaX_C [H]

EC number: NA

Molecular weight: Translated: 35297; Mature: 35166

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLAALLEGLGFGERFVRTA
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
LFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAEQPAWDGKWLLLLSEGLDK
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCH
ATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEAGVADNVICFEAQIPLALSRA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
ALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETADELTPERAFHIQLLLIHFYRRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAALAFVSEKGETSVGELPAPGSLYFQR
HCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FGGLNIEQEALCQFTR
HCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLAALLEGLGFGERFVRTA
CHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHH
LFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAEQPAWDGKWLLLLSEGLDK
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCH
ATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEAGVADNVICFEAQIPLALSRA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
ALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETADELTPERAFHIQLLLIHFYRRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAALAFVSEKGETSVGELPAPGSLYFQR
HCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FGGLNIEQEALCQFTR
HCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]