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The map label for this gene is paaF [H]

Identifier: 157160880

GI number: 157160880

Start: 1485318

End: 1486631

Strand: Direct

Name: paaF [H]

Synonym: EcHS_A1485

Alternate gene names: 157160880

Gene position: 1485318-1486631 (Clockwise)

Preceding gene: 157160879

Following gene: 157160881

Centisome position: 31.99

GC content: 51.83

Gene sequence:

>1314_bases
ATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTTACAGGCGTTACAAACACAGCGTCTGAA
ATGGACGCTCAAACACGCGTATGAAAATGTCCCGATGTATCGGCGCAAATTCAACGCAGCAGGCGTACATCCTGATGATT
TCAGGGAACTTTCAGACCTGCGTAAATTTCCCTGTACCACCAAACAGGATCTGCGCGACAACTATCCTTTCGACACCTTT
GCCGTGCCGATGGAACAAGTGGTGCGCATTCATGCTTCTTCGGGAACCACAGGTAAACCGACAGTTGTCGGCTATACGCA
AAACGATATTGATAACTGGGCCAATATTGTAGCGCGTTCGTTGCGTGCGGCGGGTGGCACGCCCAAAGACAAAATTCATG
TCGCCTATGGTTACGGGCTGTTTACTGGTGGGCTGGGTGCGCACTATGGTGCCGAACGTCTGGGCGCTACGGTGATCCCG
ATGTCTGGCGGGCAGACAGAAAAACAAGCGCAACTGATCCGTGATTTTCAACCAGATATGATCATGGTTACGCCATCTTA
TTGCCTTAACCTGATTGAAGAGCTGGAGCGGCAGTTGGGCGGTGATGCCAGCGGTTGCTCGCTGCGGGTTGGAGTGTTTG
GTGCTGAGCCGTGGACACAGGCCATGCGTAAAGAAATTGAGCGTCGCCTGGGGATCACCGCACTGGATATTTATGGCCTG
TCAGAAGTGATGGGGCCGGGGGTGGCGATGGAGTGTCTGGAAACTACCGACGGCCCAACCATTTGGGAAGATCATTTCTA
TCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCA
AAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGC
ATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGA
GATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGA
AAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATT
AAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCG
GGTGTTTGATCTGCGCAATATTGTTGGTGCCTGA

Upstream 100 bases:

>100_bases
ACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATAT
CAACCTGCGAGTACCCTACA

Downstream 100 bases:

>100_bases
CGTGTCGTCTGGCCCTGGTGAGGTAAAGCGTCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACA
ATAACAACAGACTGAATCGA

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFNAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTF
AVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRR
MDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRI
KSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA

Sequences:

>Translated_437_residues
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFNAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTF
AVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRR
MDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRI
KSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA
>Mature_437_residues
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFNAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTF
AVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRR
MDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRI
KSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=437, Percent_Identity=99.5423340961098, Blast_Score=907, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48967; Mature: 48967

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFNAAGVHPDDFRELSDL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHH
RKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARS
HHCCCCCHHHHHCCCCCCHHHHCHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHH
LRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIPMSGGQTEKQAQLIRDFQPDM
HHHCCCCCCCEEEEEEECHHHCCCCCHHCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
EEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCH
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVI
HHHHCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
RYRTRDLTRLLPGTARTMRRMDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
EVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRIKSMVGISTDVMIVNCGSIPR
EECCCCCCCEEEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SEGKACRVFDLRNIVGA
CCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFNAAGVHPDDFRELSDL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHH
RKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARS
HHCCCCCHHHHHCCCCCCHHHHCHHHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHH
LRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIPMSGGQTEKQAQLIRDFQPDM
HHHCCCCCCCEEEEEEECHHHCCCCCHHCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCE
IMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
EEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCEEEECCCH
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVI
HHHHCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
RYRTRDLTRLLPGTARTMRRMDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
EVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRIKSMVGISTDVMIVNCGSIPR
EECCCCCCCEEEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SEGKACRVFDLRNIVGA
CCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]