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The map label for this gene is uspE

Identifier: 157160844

GI number: 157160844

Start: 1443924

End: 1444874

Strand: Reverse

Name: uspE

Synonym: EcHS_A1449

Alternate gene names: 157160844

Gene position: 1444874-1443924 (Counterclockwise)

Preceding gene: 157160845

Following gene: 157160842

Centisome position: 31.12

GC content: 50.26

Gene sequence:

>951_bases
ATGGCTATGTATCAGAACATGCTCGTTGTTATCGATCCTAACCAGGACGACCAACCAGCATTGCGGCGAGCTGTTTATTT
ACATCAACGGATTGGTGGCAAAATTAAAGCCTTTTTGCCGATCTATGACTTCTCATACGAAATGACCACCCTGCTCTCCC
CGGACGAACGTACCGCTATGCGTCAGGGCGTCATCAGCCAGCGTACAGCCTGGATCCACGAGCAGGCAAAATATTATCTC
AATGCTGGCGTTCCCATTGAAATTAAAGTGGTCTGGCATAACCGTCCTTTCGAAGCCATTATTCAGGAAGTGATCAGCGG
CGGACACGATTTGGTGCTAAAAATGGCGCACCAACATGACCGTCTGGAAGCGGTGATTTTTACGCCAACGGACTGGCATC
TGTTACGCAAATGCCCAAGCCCGGTGTGGATGGTGAAAGACCAGCCGTGGCCGGAAGGAGGCAAGGCGCTGGTGGCGGTG
AATCTCGCCAGTGAAGAGCCGTATCATAATGCGCTCAATGAAAAACTGGTCAAAGAGACGATCGAACTGGCAGAACAAGT
CAACCATACCGAAGTTCATCTGGTTGGCGCTTATCCGGTAACGCCAATTAATATCGCGATTGAACTGCCGGAATTTGACC
CGAGCGTTTATAACGATGCCATTCGTGGGCAACATTTGCTGGCAATGAAAGCTCTGCGGCAGAAATTCGGCATTAATGAA
AACATGACGCACGTAGAAAAAGGTCTGCCTGAAGAGGTGATTCCTGATTTGGCGGAGCATTTACAGGCGGGAATTGTGGT
TCTGGGCACGGTCGGACGCACCGGTATTTCAGCAGCATTCCTCGGCAACACGGCGGAACAGGTGATTGATCATCTTCGCT
GCGACCTGCTGGTCATTAAACCTGACCAGTATCAGACACCCGTTGAACTGGATGACGAAGAAGACGATTAA

Upstream 100 bases:

>100_bases
TTATTGATCTGGCAGAAGGTTCATCACTGTTTCATTCACCAGATATGGGTTAATCTTTTAATTACAAACTGCGTTGACAG
TTGTTGTAAGGAGACCCTGT

Downstream 100 bases:

>100_bases
CGACTCTTCAATAACTGACAAGGCCCGAGTTATCGGGCCTATTTTTTACTTACTGCTCTTCGCCGCCAAGGAAATAAATT
CCCAACGGGATTGCCAGCAA

Product: universal stress protein UspE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDFSYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYL
NAGVPIEIKVVWHNRPFEAIIQEVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYNDAIRGQHLLAMKALRQKFGINE
NMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGISAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD

Sequences:

>Translated_316_residues
MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDFSYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYL
NAGVPIEIKVVWHNRPFEAIIQEVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYNDAIRGQHLLAMKALRQKFGINE
NMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGISAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD
>Mature_315_residues
AMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDFSYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLN
AGVPIEIKVVWHNRPFEAIIQEVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAVN
LASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYNDAIRGQHLLAMKALRQKFGINEN
MTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGISAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD

Specific function: Required for resistance to DNA-damaging agents

COG id: COG0589

COG function: function code T; Universal stress protein UspA and related nucleotide-binding proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the universal stress protein A family

Homologues:

Organism=Escherichia coli, GI1787594, Length=316, Percent_Identity=100, Blast_Score=652, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): USPE_ECO57 (P0AAC2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90868
- PIR:   G85750
- RefSeq:   NP_287853.1
- RefSeq:   NP_309941.1
- ProteinModelPortal:   P0AAC2
- SMR:   P0AAC2
- EnsemblBacteria:   EBESCT00000027590
- EnsemblBacteria:   EBESCT00000059055
- GeneID:   912394
- GeneID:   961275
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2435
- KEGG:   ecs:ECs1914
- GeneTree:   EBGT00050000011173
- HOGENOM:   HBG638855
- OMA:   YDLSYEM
- ProtClustDB:   PRK11175
- BioCyc:   ECOL83334:ECS1914-MONOMER
- GO:   GO:0005737
- InterPro:   IPR014729
- InterPro:   IPR006016
- Gene3D:   G3DSA:3.40.50.620

Pfam domain/function: PF00582 Usp

EC number: NA

Molecular weight: Translated: 35707; Mature: 35576

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDFSYEMTTLLSPDERTAM
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCHHHHH
RQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQEVISGGHDLVLKMAHQHD
HHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCHHHHHHHHHCCCHHEEHHHHHCCC
RLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAVNLASEEPYHNALNEKLVKET
CEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
IELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYNDAIRGQHLLAMKALRQKFGINE
HHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
NMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGISAAFLGNTAEQVIDHLRCDLLVIK
CHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCHHHHHHHHCCCEEEEC
PDQYQTPVELDDEEDD
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDFSYEMTTLLSPDERTAM
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCHHHHH
RQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQEVISGGHDLVLKMAHQHD
HHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCHHHHHHHHHCCCHHEEHHHHHCCC
RLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAVNLASEEPYHNALNEKLVKET
CEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
IELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYNDAIRGQHLLAMKALRQKFGINE
HHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
NMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGISAAFLGNTAEQVIDHLRCDLLVIK
CHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCHHHHHHHHCCCEEEEC
PDQYQTPVELDDEEDD
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796