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The map label for this gene is ynaI

Identifier: 157160842

GI number: 157160842

Start: 1442342

End: 1443373

Strand: Reverse

Name: ynaI

Synonym: EcHS_A1447

Alternate gene names: 157160842

Gene position: 1443373-1442342 (Counterclockwise)

Preceding gene: 157160844

Following gene: 157160840

Centisome position: 31.08

GC content: 43.22

Gene sequence:

>1032_bases
ATGATCGCTGAACTGTTTACAAATAATGCGCTTAATCTGGTCATTATTTTCGGTAGCTGCGCAGCATTGATTCTGATGAG
CTTTTGGTTTCGCCGTGGAAATCGTAAAAGAAAAGGATTTTTATTCCATGCGGTGCAATTTTTAATCTACACCATAATTA
TCAGTGCTGTTGGTAGCATTATTAATTATGTTATAGAAAACTATAAACTCAAATTTATCACCCCAGGCGTTATCGATTTT
ATCTGTACGTCCCTGATTGCGGTTATTCTGACGATTAAGCTATTTCTGCTGATTAATCAGTTTGAAAAACAGCAGATTAA
AAAAGGTCGCGATATCACCAGTGCGCGGATTATGTCGCGTATCATCAAAATCACCATTATTGTGGTGCTTGTTCTGCTTT
ATGGCGAACATTTCGGCATGAGCCTTTCTGGCTTGCTGACCTTTGGTGGTATTGGTGGTCTGGCTGTCGGTATGGCCGGT
AAAGATATTCTGAGTAACTTCTTTTCCGGGATTATGCTCTATTTCGACCGTCCTTTCAGTATTGGCGACTGGATCCGTTC
ACCGGACAGAAATATCGAAGGTACAGTAGCGGAAATTGGCTGGCGAATTACCAAAATTACGACCTTTGATAATCGTCCAT
TGTACGTACCGAACTCGCTGTTTTCGTCGATCAGCGTAGAAAACCCAGGACGAATGACCAACCGCCGCATTACCACGACC
ATTGGTTTACGTTATGAGGATGCGGCAAAAGTGGGCGTTATTGTCGAAGCTGTACGTGAGATGCTGAAAAATCACCCGGC
CATCGACCAGCGACAAACCTTACTGGTTTATTTCAACCAGTTTGCTGACTCTTCATTGAATATTATGGTTTATTGCTTTA
CCAAAACCACGGTATGGGCTGAATGGCTTGCTGCACAGCAAGACGTTTATTTGAAGATTATCGATATTGTACAGTCACAC
GGCGCGGACTTTGCCTTCCCCAGCCAGACGCTGTATATGGATAACATTACACCGCCGGAACAGGGTCGCTAA

Upstream 100 bases:

>100_bases
TTCCCTGACTATTGATGATCTGAATGAATTGTCGCGGTAAAATAGCCCCGCCAGGGGTTTCCGGCATGACGACAATTATC
TGGAGGAGCCTGATAAAACA

Downstream 100 bases:

>100_bases
ATCAGACATCACTGGCGTTATGCACAAGGGTAACGCCAGTTCCCATCACATCAATGCTTCACAATATACATAGTCCGACT
GTACGCCACATCTTCAGGAT

Product: small conductance mechanosensitive ion channel family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDF
ICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTT
IGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSH
GADFAFPSQTLYMDNITPPEQGR

Sequences:

>Translated_343_residues
MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDF
ICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTT
IGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSH
GADFAFPSQTLYMDNITPPEQGR
>Mature_343_residues
MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDF
ICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTT
IGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSH
GADFAFPSQTLYMDNITPPEQGR

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family

Homologues:

Organism=Escherichia coli, GI1787591, Length=343, Percent_Identity=100, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI1789291, Length=236, Percent_Identity=22.4576271186441, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YNAI_ECO57 (P0AEB6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85751
- PIR:   H90867
- RefSeq:   NP_287855.1
- RefSeq:   NP_309939.1
- ProteinModelPortal:   P0AEB6
- SMR:   P0AEB6
- EnsemblBacteria:   EBESCT00000029130
- EnsemblBacteria:   EBESCT00000056047
- GeneID:   912403
- GeneID:   961278
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2437
- KEGG:   ecs:ECs1912
- GeneTree:   EBGT00050000009775
- HOGENOM:   HBG710405
- OMA:   RKERKTI
- ProtClustDB:   CLSK880101
- BioCyc:   ECOL83334:ECS1912-MONOMER
- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014

Pfam domain/function: PF00924 MS_channel; SSF82689 MscC_channel_C; SSF82861 MscS_transmembr; SSF50182 Sm_like_riboprot

EC number: NA

Molecular weight: Translated: 38755; Mature: 38755

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13e00b6c)-; HASH(0x146a6c6c)-; HASH(0x142fbb40)-; HASH(0x12a166a8)-; HASH(0x143a1aa4)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSI
CCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
INYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSR
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCC
IGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTT
HHHHHCCCCCCCCCHHHHHCEEEEEEEEECCCCEECCHHHHHHCCCCCCCCCCCCEEEEE
IGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWA
EEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECHHHHH
EWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCH
>Mature Secondary Structure
MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHAVQFLIYTIIISAVGSI
CCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
INYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSR
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCC
IGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTT
HHHHHCCCCCCCCCHHHHHCEEEEEEEEECCCCEECCHHHHHHCCCCCCCCCCCCEEEEE
IGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWA
EEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECHHHHH
EWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796