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The map label for this gene is puuB

Identifier: 157160812

GI number: 157160812

Start: 1411685

End: 1412965

Strand: Direct

Name: puuB

Synonym: EcHS_A1416

Alternate gene names: 157160812

Gene position: 1411685-1412965 (Clockwise)

Preceding gene: 157160811

Following gene: 157160813

Centisome position: 30.4

GC content: 58.7

Gene sequence:

>1281_bases
ATGACCGAACATACCAGCAGTTACTACGCCGCCAGTGCGAATAAATATGCACCATTCGACACACTGAATGAGTCAATCAC
CTGCGACGTTTGCGTGGTTGGCGGCGGCTATACCGGGCTCTCCTCCGCGCTGCATCTGGCGGAAGCGGGCTTTGACGTAG
TGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGGCAACTTGTGAACTCCTACAGCCGCGAC
ATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAGGGTGGCGAGATCAT
CCGCGAACGTATCAAACGCTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGC
TCGCCACACTCGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAGCTGCTGGACGCTAACGCCATT
CGCCGTGAAGTAGCCAGCGATCGCTACACTGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCACCCGCTAAACCTCGC
CATTGGTGAAGCCGATGCCATCCGCCTGAACGGCGGGCGCGTGTATGAGCTTTCTGCGGTCACGCAGATCCAGCACACCA
CGCCCGCCGTGGTGCGTACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTACCTGGGCGAT
AAAGTGGAGCCGGAGCTGGCGAAACGCAGCATGCCATGCGGTACGCAGGTGATCACCACCGAGCGGCTGTCGGAAGATTT
AGCCCGTTCGCTGATCCCGAAAAACTACTGCGTGGAAGACTGTAATTATCTGCTTGATTACTACCGTCTTACCGCCGATA
ACCGCCTGCTGTACGGCGGCGGCGTAGTGTATGGCGCGCGCGACCCGGATGACGTTGAGCGTCTTGTGGTGCCGAAACTG
CTGAAAACCTTCCCGCAGTTGAAGGGCGTGAAAATCGACTATCGCTGGACGGGCAACTTCCTGCTGACCCTGTCGCGTAT
GCCGCAGTTTGGTCGCCTCGATACCAACATCTATTACATGCAGGGCTACAGCGGCCACGGCGTCACCTGTACTCATCTCG
CCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTC
CCCGGCGGGCGCACGCTGCGCGTACCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTA
A

Upstream 100 bases:

>100_bases
GGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCCTTGAAAAATTCACTGAACTGAAAACCATCTG
GATAAGCCTGGAGGCCTGAA

Downstream 100 bases:

>100_bases
TTTCCGATTAACAGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACCC
CGCGCGGGGTTGGCGTGATG

Product: gamma-glutamylputrescine oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTAKGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV

Sequences:

>Translated_426_residues
MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTAKGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV
>Mature_425_residues
TEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDI
DVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIR
REVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTAKGQVTAKYVIVAGNAYLGDK
VEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLL
KTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFP
GGRTLRVPFTAMGAAYYSLRDRLGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=100, Blast_Score=878, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUUB_ECOLI (P37906)

Other databases:

- EMBL:   U38543
- EMBL:   AB200320
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M38433
- PIR:   H64878
- RefSeq:   AP_001927.1
- RefSeq:   NP_415817.1
- ProteinModelPortal:   P37906
- SMR:   P37906
- STRING:   P37906
- EnsemblBacteria:   EBESCT00000001256
- EnsemblBacteria:   EBESCT00000016539
- GeneID:   945072
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1294
- KEGG:   eco:b1301
- EchoBASE:   EB1769
- EcoGene:   EG11822
- eggNOG:   COG0665
- GeneTree:   EBGT00050000011287
- HOGENOM:   HBG692171
- OMA:   NNYCVED
- ProtClustDB:   CLSK867510
- BioCyc:   EcoCyc:EG11822-MONOMER
- BioCyc:   MetaCyc:EG11822-MONOMER
- Genevestigator:   P37906
- InterPro:   IPR006076

Pfam domain/function: PF01266 DAO

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47170; Mature: 47039

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCCEEEECCCCCCCHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTAKGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCHHHEEECCCCEEEEEEEEECCCCCCC
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHH
RDRLGV
HHHHCC
>Mature Secondary Structure 
TEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCEEEECCCCCCCHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTAKGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCHHHEEECCCCEEEEEEEEECCCCCCC
KVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHH
RDRLGV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428