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The map label for this gene is rssA

Identifier: 157160740

GI number: 157160740

Start: 1337503

End: 1338408

Strand: Direct

Name: rssA

Synonym: EcHS_A1342

Alternate gene names: 157160740

Gene position: 1337503-1338408 (Clockwise)

Preceding gene: 157160737

Following gene: 157160741

Centisome position: 28.8

GC content: 50.22

Gene sequence:

>906_bases
ATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT
AAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATC
GATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGC
GGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGATCGAAAATTGTTCCCG
TCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCTCCATCTTGCTATTC
GCGCATCATGCAGTATTCCAGGACTCATGGCACCTGTTGCACATAACGGCTACTGGCTGGTTGATGGAGCAGTCGTTAAC
CCAATTCCTATTTCCCTCACGCGTGCATTGGGGGCTGATATTGTGATAGCGGTTGACCTGCAGCACGATGCTCATTTGAT
GCAACAAGATTTGCTCTCCTTTAATGTCAGTGAAGAAAATAGCGAGAATGGTGATTCTCTGCCGTGGCATGCGCGTCTGA
AAGAAAGGTTAGGCAGCATAACGACACGTCGGGCGGTGACAGCGCCAACGGCAACAGAGATTATGACCACTTCTATCCAG
GTGCTGGAGAACCGCCTTAAAAGGAACCGCATGGCAGGTGATCCGCCCGATATTCTGATTCAACCTGTTTGCCCGCAAAT
ATCTACGCTTGATTTCCATCGCGCGCACGCTGCCATTGCGGCCGGACAGCTGGCAGTGGAAAGGAAAATGGACGAACTTT
TGCCGTTGGTACGCACCAACATTTGA

Upstream 100 bases:

>100_bases
TGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCC
AGGGGAATCTTGCGGGAATA

Downstream 100 bases:

>100_bases
CCAGAATTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCA
GGGGAGAGAACATGACGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGSLVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQR
GGLLRGERVFNQYREIMPETEIENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLGSITTRRAVTAPTATEIMTTSIQ
VLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAHAAIAAGQLAVERKMDELLPLVRTNI

Sequences:

>Translated_301_residues
MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGSLVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQR
GGLLRGERVFNQYREIMPETEIENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLGSITTRRAVTAPTATEIMTTSIQ
VLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAHAAIAAGQLAVERKMDELLPLVRTNI
>Mature_301_residues
MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGSLVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQR
GGLLRGERVFNQYREIMPETEIENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLGSITTRRAVTAPTATEIMTTSIQ
VLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAHAAIAAGQLAVERKMDELLPLVRTNI

Specific function: Unknown

COG id: COG1752

COG function: function code R; Predicted esterase of the alpha-beta hydrolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 patatin domain

Homologues:

Organism=Homo sapiens, GI260656041, Length=183, Percent_Identity=32.2404371584699, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI116256487, Length=183, Percent_Identity=32.2404371584699, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI260656037, Length=183, Percent_Identity=32.2404371584699, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI260656039, Length=183, Percent_Identity=32.2404371584699, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI260656043, Length=183, Percent_Identity=32.2404371584699, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI148727335, Length=182, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI148727290, Length=182, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI226510936, Length=301, Percent_Identity=100, Blast_Score=616, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI193204778, Length=182, Percent_Identity=33.5164835164835, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71997299, Length=166, Percent_Identity=31.9277108433735, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71997289, Length=166, Percent_Identity=31.9277108433735, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323581, Length=304, Percent_Identity=28.9473684210526, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571388, Length=190, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281376913, Length=190, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSSA_ECOLI (P0AFR0)

Other databases:

- EMBL:   M64675
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B36871
- RefSeq:   AP_001860.1
- RefSeq:   NP_415750.2
- ProteinModelPortal:   P0AFR0
- SMR:   P0AFR0
- DIP:   DIP-35948N
- IntAct:   P0AFR0
- STRING:   P0AFR0
- EnsemblBacteria:   EBESCT00000001925
- EnsemblBacteria:   EBESCT00000018320
- GeneID:   945725
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1222
- KEGG:   eco:b1234
- EchoBASE:   EB2041
- EcoGene:   EG12120
- eggNOG:   COG1752
- GeneTree:   EBGT00050000009695
- HOGENOM:   HBG729555
- ProtClustDB:   PRK10279
- BioCyc:   EcoCyc:EG12120-MONOMER
- Genevestigator:   P0AFR0
- InterPro:   IPR016035
- InterPro:   IPR001423
- InterPro:   IPR002641

Pfam domain/function: PF01734 Patatin; SSF52151 Acyl_Trfase/lysoPlipase

EC number: NA

Molecular weight: Translated: 33068; Mature: 33068

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01237 UPF0028

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGSLVGAAYACDRLSALED
CCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
WVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEIENCSRRFAAVATNLSTGR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
ELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVNPIPISLTRALGADIVIAVDL
EEEEECCCEEEEEEECCCCCCCCCHHCCCCEEEECCCEECCCCHHHHHHCCCCEEEEEEC
QHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLGSITTRRAVTAPTATEIMTTSIQ
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHEECCCCHHHHHHHHHH
VLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAHAAIAAGQLAVERKMDELLPLVRTN
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
I
C
>Mature Secondary Structure
MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGSLVGAAYACDRLSALED
CCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
WVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEIENCSRRFAAVATNLSTGR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
ELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVNPIPISLTRALGADIVIAVDL
EEEEECCCEEEEEEECCCCCCCCCHHCCCCEEEECCCEECCCCHHHHHHCCCCEEEEEEC
QHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLGSITTRRAVTAPTATEIMTTSIQ
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHEECCCCHHHHHHHHHH
VLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAHAAIAAGQLAVERKMDELLPLVRTN
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8282700; 9097039; 8905232; 9278503