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The map label for this gene is dadX [H]

Identifier: 157160693

GI number: 157160693

Start: 1287318

End: 1288388

Strand: Direct

Name: dadX [H]

Synonym: EcHS_A1293

Alternate gene names: 157160693

Gene position: 1287318-1288388 (Clockwise)

Preceding gene: 157160692

Following gene: 157160696

Centisome position: 27.72

GC content: 58.08

Gene sequence:

>1071_bases
ATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCA
CGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGCTCGGGGCCACCG
ATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAA
GGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAA
AGCACTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCC
AGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTT
GCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCG
TTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATG
GCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATT
GGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCAT
TGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTGCGCACCA
TGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTT
GAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGATGTGCGCGCT
GGCGCTACGCGTCCCGGTTGTGACGGTGTAA

Upstream 100 bases:

>100_bases
AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCG
CACACAGCTAAGGAAACGAG

Downstream 100 bases:

>100_bases
CTTGTTGTAAGCCGGATCGGAGGCAACGTCTTCTGGGTGCAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGT
TAATGTGACAGAGCCATTGC

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Sequences:

>Translated_356_residues
MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
>Mature_355_residues
TRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLEG
FFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFA
EAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEIIG
VQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVE
LWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=356, Percent_Identity=99.7191011235955, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI1790487, Length=346, Percent_Identity=42.485549132948, Blast_Score=278, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 38845; Mature: 38714

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
TRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]