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The map label for this gene is ycdT [H]

Identifier: 157160550

GI number: 157160550

Start: 1155880

End: 1157310

Strand: Direct

Name: ycdT [H]

Synonym: EcHS_A1143

Alternate gene names: 157160550

Gene position: 1155880-1157310 (Clockwise)

Preceding gene: 157160544

Following gene: 157160552

Centisome position: 24.89

GC content: 38.16

Gene sequence:

>1431_bases
TTGACAACAGTACAGTTCTTATATCTATTAATAATAGAAAGGGATCTACAACCTACAGATTGGTGTAGCTTTATGGAAAA
AGACTATTTGAGAATTAGTAGTACTGTATTAGTGAGCTTATTGTTTGGGCTTGCTTTGGTTTTGGTGAATAGTTGGTTTA
ATCAGCCAGGCGTTGAGGAAGTTGTTCCACGATCAACCTATCTGATGGTAATGATTGCTTTATTTTTTATCGATACTGTT
GCATTTATTTTTATGCAGTTGTATTTCATTTATGACCGTAGGCAATTTTCAAACTGTGTACTTAGTTTGGCTTTTCTGAG
TTGTTTGATTTACTTTGTTAAAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAA
ATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGT
GAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGAT
AGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGG
TTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTA
CGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGTTATTTATGAGCAG
GTATAGCGTATCCACTTGGTATATTAGTCGCACTATTGAAGTAGTCAGTAAGTTAACCGTTATGGTAATATTTATGTGCC
ATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTT
TTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAA
AAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATAC
GACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTCTTGCTGACGGACATCGATACTGAACGTGCGAAA
GCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGAC
GATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTT
ACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAA

Upstream 100 bases:

>100_bases
CAAGCCTGGCTTGTTGCAAACAATGTATAAAGCACTTAGGCAATAATAATTACATTCAGCAACTATCATCATCGGTATTG
TTTGTGGGCGGAATTTCATA

Downstream 100 bases:

>100_bases
AGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGG
CCGCCACACCAACTGTGCCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTV
AFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEERLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
ENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGL
RYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYF
FNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAK
ALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP

Sequences:

>Translated_476_residues
MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTV
AFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEERLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
ENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGL
RYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYF
FNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAK
ALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP
>Mature_475_residues
TTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVA
FIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEERLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSE
NTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLR
YDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFF
NELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKA
LAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=452, Percent_Identity=99.7787610619469, Blast_Score=924, Evalue=0.0,
Organism=Escherichia coli, GI1788085, Length=449, Percent_Identity=37.4164810690423, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=35.2941176470588, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=35.1190476190476, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=34.3373493975904, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=32.183908045977, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=217, Percent_Identity=31.3364055299539, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=33.9506172839506, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081974, Length=170, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=29.4478527607362, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787056, Length=245, Percent_Identity=28.1632653061224, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 54714; Mature: 54582

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIIEERLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVFGGPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGL
HHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
RYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQ
HHHHHHHCCHHHHHHCCCCEEHHHEEECCCCCCCCEEEEEEEHHHHHHCCCCCCCCCHHH
VIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEY
HHHHHHHHHCCCCCHHHHHHHCCCCHHCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
AIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP
CCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEE
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIIEERLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVFGGPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGL
HHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
RYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQ
HHHHHHHCCHHHHHHCCCCEEHHHEEECCCCCCCCEEEEEEEHHHHHHCCCCCCCCCHHH
VIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEY
HHHHHHHHHCCCCCHHHHHHHCCCCHHCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
AIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP
CCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]