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The map label for this gene is pgaD

Identifier: 157160545

GI number: 157160545

Start: 1148405

End: 1148818

Strand: Reverse

Name: pgaD

Synonym: EcHS_A1136

Alternate gene names: 157160545

Gene position: 1148818-1148405 (Counterclockwise)

Preceding gene: 157160546

Following gene: 157160539

Centisome position: 24.74

GC content: 44.2

Gene sequence:

>414_bases
ATGAACAATTTAATTATTACGACCCGACAATCACCAGTACGTTTACTGGTTGATTATGTTGCCACAACCATCTTGTGGAC
ATTATTTGCGTTGTTCATATTCTTATTCGCCATGGATCTGCTGACGGGTTATTACTGGCAAAGCGAGGCCAGAAGCCGAC
TTCAGTTCTATTTTTTGCTGGCAGTGGCGAATGCCGTCGTGTTAATTGTCTGGGCGCTGTACAATAAGCTGCGTTTTCAA
AAACAGCAGCATCATGCAGCCTACCAATATACGCCGCAAGAATATGCAGAGAGCTTAGCAATACCTGATGAGCTCTATCA
GCAACTACAAAAAAGCCACAGGATGAGCGTACACTTCACCAGCCAGGGGCAAATAAAAATGGTTGTTTCAGAAAAAGCGC
TAGTCCGGGCATAA

Upstream 100 bases:

>100_bases
CTGGCAACGACATTGGTATCATTTACACGAGTCATGTTGATGCCTAAAAAGCAACGCGCCCGTTGGGTAAGTCCCGATCG
CGGGATTCTGAGAGGTTAAT

Downstream 100 bases:

>100_bases
ACACCCAAAACAAAGCCCGGTTCGCCCGGGCTCTGCACCGATAACACACTTAACTGTAGGCATGCAGCGTACGTTGGCAA
AGTGCCGAACGTACGCAGTC

Product: biofilm PGA synthesis protein PgaD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGYYWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQ
KQQHHAAYQYTPQEYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA

Sequences:

>Translated_137_residues
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGYYWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQ
KQQHHAAYQYTPQEYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA
>Mature_137_residues
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGYYWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQ
KQQHHAAYQYTPQEYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA

Specific function: Required for the synthesis of poly-beta-1,6-N-acetyl-D- glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787258, Length=137, Percent_Identity=100, Blast_Score=280, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PGAD_ECO57 (P69433)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85647
- PIR:   C90787
- RefSeq:   NP_287028.1
- RefSeq:   NP_309294.1
- ProteinModelPortal:   P69433
- EnsemblBacteria:   EBESCT00000026762
- EnsemblBacteria:   EBESCT00000059356
- GeneID:   912758
- GeneID:   959159
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1523
- KEGG:   ecs:ECs1267
- GeneTree:   EBGT00050000011617
- HOGENOM:   HBG469158
- OMA:   IFLFAID
- ProtClustDB:   PRK14585
- BioCyc:   ECOL83334:ECS1267-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16082; Mature: 16082

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1403a6c4)-; HASH(0x13a68d64)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGYYWQSEARSRLQFYFLL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIPDELYQQLQKSHRMSVHFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEC
SQGQIKMVVSEKALVRA
CCCCEEEEECCHHHHCH
>Mature Secondary Structure
MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGYYWQSEARSRLQFYFLL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIPDELYQQLQKSHRMSVHFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEC
SQGQIKMVVSEKALVRA
CCCCEEEEECCHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796