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The map label for this gene is hyaF

Identifier: 157160496

GI number: 157160496

Start: 1098363

End: 1099220

Strand: Direct

Name: hyaF

Synonym: EcHS_A1085

Alternate gene names: 157160496

Gene position: 1098363-1099220 (Clockwise)

Preceding gene: 157160495

Following gene: 157160497

Centisome position: 23.65

GC content: 54.9

Gene sequence:

>858_bases
ATGAGCGAAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGAT
CACCTGTCAGGTGAATGATGAACCGAGTATGGCGGCCCTGGAGCAATGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAA
ACGAGTTACAACATCAACTAAGCGAACGCCAACCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCC
GACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAG
TGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGG
AGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCC
ATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAG
CATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGA
TTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACC
TTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGA
CTCTGCGCAGCGGCTTAGCGAGGTATGTCAGTGGCTGGCGGAAGCTGCACCGACGTAA

Upstream 100 bases:

>100_bases
CCGGCGGAAACTATCGCGGCGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAA
CCGCAGCAGGAGCGTGCCTC

Downstream 100 bases:

>100_bases
AAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGA
GGGAGATCTACCATTATCGG

Product: hydrogenase-1 operon protein HyaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNA
DDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT

Sequences:

>Translated_285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNA
DDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT
>Mature_284_residues
SETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNAD
DRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPI
DGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTL
KGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT

Specific function: Not known. Could enhance the incorporation of nickel to the hydrogenase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family

Homologues:

Organism=Escherichia coli, GI1787211, Length=285, Percent_Identity=100, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYAF_ECOLI (P19932)

Other databases:

- EMBL:   M34825
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JV0077
- RefSeq:   AP_001606.1
- RefSeq:   NP_415496.1
- ProteinModelPortal:   P19932
- STRING:   P19932
- EnsemblBacteria:   EBESCT00000002100
- EnsemblBacteria:   EBESCT00000017580
- GeneID:   945572
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0959
- KEGG:   eco:b0977
- EchoBASE:   EB0468
- EcoGene:   EG10473
- eggNOG:   NOG09293
- GeneTree:   EBGT00050000009823
- HOGENOM:   HBG494754
- OMA:   GEIIEAY
- ProtClustDB:   CLSK879889
- BioCyc:   EcoCyc:EG10473-MONOMER
- Genevestigator:   P19932
- InterPro:   IPR006894

Pfam domain/function: PF04809 HupH_C

EC number: NA

Molecular weight: Translated: 31431; Mature: 31300

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
CCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT
HCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
CCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT
HCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503