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The map label for this gene is 157160337

Identifier: 157160337

GI number: 157160337

Start: 922050

End: 924464

Strand: Direct

Name: 157160337

Synonym: EcHS_A0918

Alternate gene names: NA

Gene position: 922050-924464 (Clockwise)

Preceding gene: 157160336

Following gene: 157160338

Centisome position: 19.86

GC content: 52.75

Gene sequence:

>2415_bases
ATGAGTCATGCCGATATGAACAACTGCTGCGGCTTTAACGAGGCTGCCGCAGCGTTCTCATGGAACAGCCCGAAAAAGGC
CATTAACCCTTATCTGGACCCGGCGGAAGTTGCGCCGGTTTCTACGCTTTCAAACCTGATCACTCTGTACGCTGCCGATA
ACGAGCAGGAACAGTTGCGCCGCGAGGCACTGAGTGATCAGGTCTGGGAGCGTTATTTCTTTAATGAATCACGTGATCCT
GTCCAGCGCGAAATGGAGCAGGATAAGCTCATTAGCCGGGCAAAGCTGGCGCATGAGCAGCAGCGTTTTAATTCAGACAT
GGTCATTCTGGCGGACGTCAACGCCCAGCCTTCCCATATCAGCAAGCCGCTGATGCAACGTATTGAATACTTCAGCAGCC
TGGGCAGGCCAAAGGCTTATTCCCGCTATTTACGTGAGACGATTAAGCCATGTCTGGAACGACTGGAGCATGTACGCGAC
AGTCAGCTATCTGCATCTTTTCGCTTTATGGCAAGCCATGAAGGGCTGGACGGCCTGCTGATCCTGCCTGAAATGAGTCA
GGATCAGGTGAAACGCCTGTCCACCTTGGTAGCTGCGCATATGAGTATGTGCCTTGATGCAGCTTGTGGTGATTTGTATG
CCACCGATGACGTTAAGCCAGAAGAAATCCGCAAGACATGGGAAAAGGTGGCAGCGGAAACCCTGCGTCTGGATGTCATC
CCGCCTGCGTTTGAGCAACTCCGTCGGAAAAGAAACCGCCGTAAACCCGTGCCCTATGAACTCATTCCGGGTTCGCTGGC
GCGTATGCTGTGCGCCGACTGGTGGTATCGGAAATTGTGGAAGATGCGTTGCGAATGGCGGGAAGAGCAGTTGCGTGCTG
TCTGCCTGGTCAGCAAAAAAGCATCTCCCTATGTCAGCTATGAAGCCGTGATGCATAAACGTGAGCAGCGCCGTAAGTCA
CTGGAGTTTTTCCGTTCTCATGAACTGGTGAACGAAGACGGAGACGCGCTGGATATGGAAGATGTGGTAAACGCCAGCAG
CAGCAACCCGGCGCATCGTCGCAATGAGATGATGGCCTGTGTTAAAGGTCTGGAGCTTATCGCGGAAATGCGCGGTGACT
GCGCCGTTTTCTACACCATTACCTGTCCGTCACGTTTCCATTCCACGCTCAATAACGGCAGACCCAACCCGACATGGACA
AATGCGACGGTAAGACAAAGCAGCGATTATCTGGTCGGTACGTTTGCTGCATTTCGTAAGGCGATGCACAAAGCCGGGTT
GCGCTGGTATGGCGTGCGGGTGGCTGAGCCGCATCATGACGGCACAGTTCACTGGCACCTGTTGTGTTTCATGCGCAAAA
AAGACCGCCGCGCCATTACTGCATTGTTGCGTAAGTTTGCTATCCGTGAAGACCGTGAGGAGCTGGGCAATAACACGGGG
CCGCGCTTTAAGTCTGAGCTGATTAACCCGCGAAAAGGAACGCCGACAAGCTACATCGCGAAATACATCAGTAAGAACAT
TGATGGGCGTGGTCTGGCTGGCGAAATCAGCAAGGAAACGGGTAAATCCCTGCGTGATAACGCTGAATACGTTAATGCCT
GGGCGTCTCTGCATCGTGTTCAGCAATTTCGCTTCTTTGGTATTCCGGGGCGTCAGGCGTACCGTGAATTGCGATTGCTG
GCTGGTCAGGCGGCAAGGCAACAGGGTGACAAAAAAGCAGGTGCGCCGGTACTGGATAACCCGCGCCTTGATGCCATTCT
GGCTGCTGCTGATGCTGGTTGTTTTGCCACCTACATTATGAAGCAGGGCGGCGTACTGGTTCCCCGCAAATATCACCTTA
TCAGAACAGCTTATGAAATTAACGAAGAGCCGACCGCCTATGGCGATCACGGTATTCGTATTTATGGCATCTGGTCACCC
ATTGCAGAGGGCAAGATTTGTACTCATGCAGTGAAGTGGAAAATGGTTCGTAAGGCCGTTGACGTTCAGGAGGCGGCAGC
CGACCAGGGCGCTTGCGCCCCTTGGACTCGTGGCAATAACTGTCCCCTTGCTGAAAATTTGAACCAACAGGAGAAAGATA
AATCAGCTGATGGGGACCCCAGAACGGACTTTACCCGCATGGATGACAAGGAGTTGCACGATTACCTGCACAGTATGAAC
AAAAAGGAGCGCCGGGAACTGGCTGCAAGGTTACGTCTGGTGAAACCGAAACGGCGTAGAGACTACAAACAGCGAATTAC
AGACCATCAACGACAGCAGCTCGTCTATGAACTGAAATCCAGAGGATTTGATGGCAGCGAGAAAGAGGTCGATTTACTCC
TTCGCGGCGGCAGTATTCCGTCAGGAGCAGGCCTGCGTATCTTCTATCGGAACCAGCGTTTGCAGGAAGATGATAAGTGG
CGGAACCTGTATTAA

Upstream 100 bases:

>100_bases
AATCAGCAACAGAAATCATTGCTGTTTCCGGGCCGCGCTGCTGGATGGGATTTGATTATTCGCGTGGCGTGGATAGTTCT
GCCGTGTACGGAGTACGTGC

Downstream 100 bases:

>100_bases
TTACGCTGGTTAACAATTCGTGCTCTTAATAATACCAGGCATATCAAGCTGATAAGCGTAAAAAAAACGTTTTACATCAG
TAAGATTATTATATACTGTA

Product: putative replication gene A protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLSNLITLYAADNEQEQLRREALSDQVWERYFFNESRDP
VQREMEQDKLISRAKLAHEQQRFNSDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
SQLSASFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEEIRKTWEKVAAETLRLDVI
PPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKS
LEFFRSHELVNEDGDALDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWT
NATVRQSSDYLVGTFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLRKFAIREDREELGNNTG
PRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLL
AGQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIWSP
IAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLNQQEKDKSADGDPRTDFTRMDDKELHDYLHSMN
KKERRELAARLRLVKPKRRRDYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDDKW
RNLY

Sequences:

>Translated_804_residues
MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLSNLITLYAADNEQEQLRREALSDQVWERYFFNESRDP
VQREMEQDKLISRAKLAHEQQRFNSDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
SQLSASFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEEIRKTWEKVAAETLRLDVI
PPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKS
LEFFRSHELVNEDGDALDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWT
NATVRQSSDYLVGTFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLRKFAIREDREELGNNTG
PRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLL
AGQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIWSP
IAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLNQQEKDKSADGDPRTDFTRMDDKELHDYLHSMN
KKERRELAARLRLVKPKRRRDYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDDKW
RNLY
>Mature_803_residues
SHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLSNLITLYAADNEQEQLRREALSDQVWERYFFNESRDPV
QREMEQDKLISRAKLAHEQQRFNSDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDS
QLSASFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEEIRKTWEKVAAETLRLDVIP
PAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSL
EFFRSHELVNEDGDALDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWTN
ATVRQSSDYLVGTFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLRKFAIREDREELGNNTGP
RFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLA
GQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIWSPI
AEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLNQQEKDKSADGDPRTDFTRMDDKELHDYLHSMNK
KERRELAARLRLVKPKRRRDYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDDKWR
NLY

Specific function: Possible endonuclease which induces a single-strand cut and initiates DNA replication (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phage GPA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020220
- InterPro:   IPR008766
- ProDom:   PD092055 [H]

Pfam domain/function: PF05840 Phage_GPA [H]

EC number: NA

Molecular weight: Translated: 92461; Mature: 92330

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLSNLITLYAADNEQEQLR
CCCCCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHH
REALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQRFNSDMVILADVNAQPSHI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
SKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDSQLSASFRFMASHEGLDGLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
ILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEEIRKTWEKVAAETLRLDVI
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQLRAVCLVSKK
CHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDALDMEDVVNASSSNPAHRRNEMMAC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHH
VKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWTNATVRQSSDYLVGTFAAFRK
HHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLRKFAIREDREELGNNTG
HHHHCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGKSLRDNAEYVNAWASLHRV
CCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
QQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIM
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHH
KQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAV
HCCCEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
DVQEAAADQGACAPWTRGNNCPLAENLNQQEKDKSADGDPRTDFTRMDDKELHDYLHSMN
HHHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
KKERRELAARLRLVKPKRRRDYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
SGAGLRIFYRNQRLQEDDKWRNLY
CCCCEEEEEECCCCCCCHHHCCCC
>Mature Secondary Structure 
SHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLSNLITLYAADNEQEQLR
CCCCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHH
REALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQRFNSDMVILADVNAQPSHI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
SKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDSQLSASFRFMASHEGLDGLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
ILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEEIRKTWEKVAAETLRLDVI
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQLRAVCLVSKK
CHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDALDMEDVVNASSSNPAHRRNEMMAC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHH
VKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWTNATVRQSSDYLVGTFAAFRK
HHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLRKFAIREDREELGNNTG
HHHHCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGKSLRDNAEYVNAWASLHRV
CCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
QQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIM
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHH
KQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAV
HCCCEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
DVQEAAADQGACAPWTRGNNCPLAENLNQQEKDKSADGDPRTDFTRMDDKELHDYLHSMN
HHHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
KKERRELAARLRLVKPKRRRDYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
SGAGLRIFYRNQRLQEDDKWRNLY
CCCCEEEEEECCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]