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The map label for this gene is dam [C]

Identifier: 157160336

GI number: 157160336

Start: 921196

End: 922053

Strand: Direct

Name: dam [C]

Synonym: EcHS_A0917

Alternate gene names: 157160336

Gene position: 921196-922053 (Clockwise)

Preceding gene: 157160335

Following gene: 157160337

Centisome position: 19.84

GC content: 46.5

Gene sequence:

>858_bases
ATGAGCACCATCCTGAAATGGGCGGGAAATAAAACCGCCATTATGTCCGAACTGAAAAAACATCTTCCTGCTGGCCCGCG
ACTGGTTGAACCTTTCGCGGGGTCCTGTGCTGTGATGATGGAGACGGATTATCCCAGCTATCTGGTTGCGGATATTAATC
CTGATTTAATCAACCTCTATAAAAAGGTTGCCGCTGATTGTGAATCGTTTATATCTCGCGCCAGAGTTTTATTTGAGATC
GCAAACAGGGAGGTGGCTTATTACAACATAAGGCAGGAGTTTAATTACTCAACTGAAATTACTGATTTCATGAAAGCGGT
ATATTTCCTGTATCTCAATCGTCACGGTTACCGTGGTTTATGTCGCTATAACAAGAGCGGGCATTTCAACATTCCCTACG
GTAATTATAAAAATCCGTATTTCCCTGAAAAAGAAATTCGCGCATTTGCAGAAAAGGCCCAGCGAGCAACGTTTATCTGC
GCCAGCTTTGATGAAACGCTGGCGATGTTGAAGGCGGGAGATGTGGTGTATTGCGATCCGCCGTATGACGGTACGTTTTC
CGGCTATCACACTGATGGTTTCACTGAAGATGACCAGTATCACCTGGCATCCGTTCTTGAACATCGGTCATCAGAAGGAC
ATCCGGTCATTGTTTCTAACAGTGACACATCCCTGATCCGTTCGCTGTATCGCAATTTTACTCACCACTATATCAAGGTA
AAACGCAGCATCGGTGTGGCAGCTGGCGAGGGTAAATCAGCAACAGAAATCATTGCTGTTTCCGGGCCGCGCTGCTGGAT
GGGATTTGATTATTCGCGTGGCGTGGATAGTTCTGCCGTGTACGGAGTACGTGCATGA

Upstream 100 bases:

>100_bases
TTCCGCCAGCACGCCGCCGCGCCATTCCGGGTGTGCAGCTTTGCATTACCTGTCAGGAAATCGCAGAGCTGAAAGGCAAA
CATTACAACGGAGGTGTTGT

Downstream 100 bases:

>100_bases
GTCATGCCGATATGAACAACTGCTGCGGCTTTAACGAGGCTGCCGCAGCGTTCTCATGGAACAGCCCGAAAAAGGCCATT
AACCCTTATCTGGACCCGGC

Product: retron adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDLINLYKKVAADCESFISRARVLFEI
ANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKV
KRSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVDSSAVYGVRA

Sequences:

>Translated_285_residues
MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDLINLYKKVAADCESFISRARVLFEI
ANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKV
KRSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVDSSAVYGVRA
>Mature_284_residues
STILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDLINLYKKVAADCESFISRARVLFEIA
NREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICA
SFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVK
RSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVDSSAVYGVRA

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=267, Percent_Identity=46.8164794007491, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32210; Mature: 32079

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDLINLY
CCCCCHHCCCHHHHHHHHHHHCCCCCCEECCCCCCEEEEEECCCCCEEEECCCHHHHHHH
KKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGL
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
CRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDP
EEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCEEEECC
PYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKV
CCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEE
KRSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVDSSAVYGVRA
HHHCCEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
STILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDLINLY
CCCCHHCCCHHHHHHHHHHHCCCCCCEECCCCCCEEEEEECCCCCEEEECCCHHHHHHH
KKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGL
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
CRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDP
EEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCEEEECC
PYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKV
CCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEE
KRSIGVAAGEGKSATEIIAVSGPRCWMGFDYSRGVDSSAVYGVRA
HHHCCEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]