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The map label for this gene is galK [H]

Identifier: 157160232

GI number: 157160232

Start: 815433

End: 816581

Strand: Reverse

Name: galK [H]

Synonym: EcHS_A0811

Alternate gene names: 157160232

Gene position: 816581-815433 (Counterclockwise)

Preceding gene: 157160233

Following gene: 157160231

Centisome position: 17.59

GC content: 53.09

Gene sequence:

>1149_bases
ATGAGTCTGAAAGAAAAAACACAATCTCTGTTTGCCAACGCATTTGGCTACCCTGCCACTCACACCATTCAGGCGCCTGG
CCGCGTGAATTTGATTGGTGAACACACCGACTACAACGACGGTTTCGTTCTGCCCTGCGCGATTGATTATCAAACCGTGA
TCAGTTGTGCACCACGCGATGACCGTAAAGTTCGCGTGATGGCAGCCGATTATGAAAATCAGCTCGACGAGTTTTCCCTC
GATGCGCCCATTGTCGCACATGAAAACTATCAATGGGCTAACTACGTTCGTGGCGTGGTGAAACATCTGCAACTGCGTAA
CAACAGCTTCGGCGGCGTGGACATGGTGATCAGCGGCAATGTGCCGCAGGGTGCCGGGTTAAGTTCTTCCGCTTCACTGG
AAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAA
GCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTT
GCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACT
TCAAACGTACCCTGGTTGGCAGCGAATACAACACCCGTCGTGAACAGTGCGAAACCGGTGCGCGTTTCTTCCAGCAGCCA
GCCCTGCGTGATGTCACCATTGAAGAGTTCAACGCTGTTGCGCATGAACTGGACCCGATCGTGGCGAAACGCGTGCGGCA
TATCCTGACTGAAAACGCCCGCACCGTTGAAGCTGCCAGCGCGCTGGAGCAGGGCGACCTGAAACGTATGAGCGAGTTGA
TGGCGGAGTCTCATGCCTCTATGCGCGATGATTTCGAAATCACCGTGCCGCAAATTGACACTCTGGTAGAAATCGTCAAA
GCTGTGATTGGCGACAAAGGTGGCGTACGCATGACCGGCGGCGGATTTGGCGGCTGTATCGTCGCGTTGATCCCGGAAGA
GCTGGTGCCTGCCGTACAGCAAGCTGTCGCTGAACAATATGAAGCAAAAACAGGTATTAAAGAGACTTTTTACGTTTGTA
AACCATCACAAGGAGCAGGACAGTGCTGA

Upstream 100 bases:

>100_bases
AATGCTGGCAGAGACCCAGCGAGACCTGACCGCAGAACAGGCAGCAGAGCGTTTGCGCGCAGTCAGCGATATCCATTTTC
GCGAATCCGGAGTGTAAGAA

Downstream 100 bases:

>100_bases
ACGAAACTCCCGCACTGGCACCCGATGGTCAGCCGTACCGACTGTTAACTTTGCGTAACAACGCAGGGATGGTAGTCACG
CTGATGGACTGGGGTGCGAC

Product: galactokinase

Products: NA

Alternate protein names: Galactose kinase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSL
DAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQP
ALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMSELMAESHASMRDDFEITVPQIDTLVEIVK
AVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC

Sequences:

>Translated_382_residues
MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSL
DAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQP
ALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMSELMAESHASMRDDFEITVPQIDTLVEIVK
AVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC
>Mature_381_residues
SLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLD
APIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEA
ENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQPA
LRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMSELMAESHASMRDDFEITVPQIDTLVEIVKA
VIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC

Specific function: Galactose metabolism; first step. [C]

COG id: COG0153

COG function: function code G; Galactokinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. GalK subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503895, Length=379, Percent_Identity=44.5910290237467, Blast_Score=284, Evalue=8e-77,
Organism=Homo sapiens, GI48527957, Length=439, Percent_Identity=26.879271070615, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4503897, Length=439, Percent_Identity=26.879271070615, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786972, Length=382, Percent_Identity=99.738219895288, Blast_Score=790, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71989053, Length=441, Percent_Identity=25.8503401360544, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319494, Length=264, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320212, Length=186, Percent_Identity=29.5698924731183, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24661292, Length=412, Percent_Identity=25.4854368932039, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355577, Length=412, Percent_Identity=25.4854368932039, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24661285, Length=412, Percent_Identity=25.4854368932039, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000705
- InterPro:   IPR022963
- InterPro:   IPR019741
- InterPro:   IPR019539
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR006206
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF10509 GalKase_gal_bdg; PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.6 [H]

Molecular weight: Translated: 41473; Mature: 41341

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00106 GALACTOKINASE ; PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRD
CCHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCCEEEEEEECHHHHHCCCCCC
DRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGN
CCEEEEEECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
VPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISAL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCCCCHHHHHHHHH
GKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQP
CCCCCEEEEEHHHCCCHHCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCC
ALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMSELMAESHAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
MRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQY
CCCCCEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHHH
EAKTGIKETFYVCKPSQGAGQC
HHHCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
SLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRD
CHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCCEEEEEEECHHHHHCCCCCC
DRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGN
CCEEEEEECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
VPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISAL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCCCCHHHHHHHHH
GKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQP
CCCCCEEEEEHHHCCCHHCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCC
ALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMSELMAESHAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
MRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQY
CCCCCEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHHH
EAKTGIKETFYVCKPSQGAGQC
HHHCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA