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The map label for this gene is galM

Identifier: 157160231

GI number: 157160231

Start: 814399

End: 815439

Strand: Reverse

Name: galM

Synonym: EcHS_A0810

Alternate gene names: 157160231

Gene position: 815439-814399 (Counterclockwise)

Preceding gene: 157160232

Following gene: 157160230

Centisome position: 17.56

GC content: 55.52

Gene sequence:

>1041_bases
GTGCTGAACGAAACTCCCGCACTGGCACCCGATGGTCAGCCGTACCGACTGTTAACTTTGCGTAACAACGCAGGGATGGT
AGTCACGCTGATGGACTGGGGTGCGACTTTACTTTCAGCCCGTATTCCGCTTTCCGATGGCAGCGTCCGCGAGGCGCTGC
TCGGCTGTGCCAGCCCGGAATGCTATCAGGATCAGGCCGCGTTTCTGGGGGCCTCTATTGGTCGTTATGCCAACCGTATC
GCCAATAGCCGTTATACCTTTGACGGTGAAACCGTGACCCTTTCGCCAAGTCAGGGCGTTAACCAGCTGCACGGCGGCCC
GGAAGGGTTCGACAAACGTCGCTGGCAGATTGTGAACCAGAACGATCGTCAGGTGCTGTTTGCCCTGAGTTCAGATGATG
GCGATCAGGGCTTCCCGGGTAATCTCGGCGCAACGGTGCAATATCGTCTGACCGACGATAACCGTATCTCCATTACTTAT
CGCGCCACAGTTGATAAACCTTGCCCGGTGAATATGACTAATCACGTCTATTTCAATCTTGACGGCGAGCAGTCTGACGT
GCGCAATCACAAGTTGCAGATTCTGGCGGACGAATATCTGCCGGTTGATGAAGGCGGCATTCCGCACGACGGCCTGAAAT
CTGTCGCCGGAACGTCTTTTGATTTCCGCAGCGCCAAAATCATCGCCAGTGAGTTTCTTGCCGACGACGATCAGCGCAAA
GTGAAAGGTTACGATCACGCATTCTTGTTACAGGCCAAAGGCGATGGCAAGAAAGTGGCGGCGCATGTCTGGTCAGCAGA
TGAAAAATTGCAGCTGAAGGTCTACACCACCGCTCCGGCTCTTCAATTCTACTCCGGCAACTTCCTCGGCGGCACACCGT
CGCGGGGAACCGAACCTTACGCCGACTGGCAAGGGCTGGCGTTGGAAAGCGAGTTTCTGCCGGACAGCCCGAACCACCCT
GAATGGCCGCAACCGGACTGCTTCCTGCGTCCTGGCGAAGAGTATTCCAGCCTGACGGAATATCAGTTTATTGCTGAGTA
A

Upstream 100 bases:

>100_bases
TGGTGCCTGCCGTACAGCAAGCTGTCGCTGAACAATATGAAGCAAAAACAGGTATTAAAGAGACTTTTTACGTTTGTAAA
CCATCACAAGGAGCAGGACA

Downstream 100 bases:

>100_bases
TGTTAAGCCCTCGTCATGAGGGCTTTATCTTCATATTGTTCAAATCACCAGCAAACACCGACATATTTGCAACTCAATAT
TCACAACAACCTTACACTGC

Product: aldose 1-epimerase

Products: NA

Alternate protein names: Galactose mutarotase; Type-1 mutarotase

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPLSDGSVREALLGCASPECYQDQAAFLGASIGRYANRI
ANSRYTFDGETVTLSPSQGVNQLHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGTSFDFRSAKIIASEFLADDDQRK
VKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYTTAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHP
EWPQPDCFLRPGEEYSSLTEYQFIAE

Sequences:

>Translated_346_residues
MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPLSDGSVREALLGCASPECYQDQAAFLGASIGRYANRI
ANSRYTFDGETVTLSPSQGVNQLHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGTSFDFRSAKIIASEFLADDDQRK
VKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYTTAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHP
EWPQPDCFLRPGEEYSSLTEYQFIAE
>Mature_346_residues
MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPLSDGSVREALLGCASPECYQDQAAFLGASIGRYANRI
ANSRYTFDGETVTLSPSQGVNQLHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGTSFDFRSAKIIASEFLADDDQRK
VKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYTTAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHP
EWPQPDCFLRPGEEYSSLTEYQFIAE

Specific function: Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose

COG id: COG2017

COG function: function code G; Galactose mutarotase and related enzymes

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldose epimerase family

Homologues:

Organism=Homo sapiens, GI20270355, Length=322, Percent_Identity=37.5776397515528, Blast_Score=202, Evalue=5e-52,
Organism=Escherichia coli, GI1786971, Length=346, Percent_Identity=100, Blast_Score=719, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17557428, Length=329, Percent_Identity=32.8267477203647, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI115533334, Length=329, Percent_Identity=32.8267477203647, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6319493, Length=348, Percent_Identity=25, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322004, Length=357, Percent_Identity=27.7310924369748, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324399, Length=352, Percent_Identity=24.7159090909091, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24668278, Length=338, Percent_Identity=33.7278106508876, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24659048, Length=342, Percent_Identity=34.5029239766082, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24583720, Length=336, Percent_Identity=32.1428571428571, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24641876, Length=354, Percent_Identity=27.9661016949153, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24668282, Length=340, Percent_Identity=28.5294117647059, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GALM_ECOLI (P0A9C3)

Other databases:

- EMBL:   U13636
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64811
- RefSeq:   AP_001387.1
- RefSeq:   NP_415277.1
- ProteinModelPortal:   P0A9C3
- SMR:   P0A9C3
- DIP:   DIP-35901N
- MINT:   MINT-1249454
- STRING:   P0A9C3
- SWISS-2DPAGE:   P0A9C3
- 2DBase-Ecoli:   P0A9C3
- EnsemblBacteria:   EBESCT00000000736
- EnsemblBacteria:   EBESCT00000000737
- EnsemblBacteria:   EBESCT00000000738
- EnsemblBacteria:   EBESCT00000015491
- GeneID:   944943
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0739
- KEGG:   eco:b0756
- EchoBASE:   EB1649
- EcoGene:   EG11698
- eggNOG:   COG2017
- GeneTree:   EBGT00050000010070
- HOGENOM:   HBG703640
- OMA:   SPNHPDW
- ProtClustDB:   PRK11055
- BioCyc:   EcoCyc:ALDOSE1EPIM-MONOMER
- BioCyc:   MetaCyc:ALDOSE1EPIM-MONOMER
- Genevestigator:   P0A9C3
- GO:   GO:0005737
- InterPro:   IPR018052
- InterPro:   IPR013458
- InterPro:   IPR008183
- InterPro:   IPR015443
- InterPro:   IPR011013
- InterPro:   IPR014718
- Gene3D:   G3DSA:2.70.98.10
- PANTHER:   PTHR10091
- TIGRFAMs:   TIGR02636

Pfam domain/function: PF01263 Aldose_epim; SSF74650 Gal_mut_like

EC number: =5.1.3.3

Molecular weight: Translated: 38191; Mature: 38191

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00545 ALDOSE_1_EPIMERASE

Important sites: ACT_SITE 175-175 ACT_SITE 309-309 BINDING 79-79 BINDING 245-245

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPLSDGSVREALLGCASPE
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCEEHEEECCCCCCHHHHHHHHCCCCH
CYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQLHGGPEGFDKRRWQIVNQ
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCHHHHCCCCCCCCCCEEEEECC
NDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITYRATVDKPCPVNMTNHVYFNL
CCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCEEEEEE
DGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGTSFDFRSAKIIASEFLADDDQRK
CCCHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHH
VKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYTTAPALQFYSGNFLGGTPSRGTEPY
HCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCCCC
ADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEEYSSLTEYQFIAE
CCCCCEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHCEEEECC
>Mature Secondary Structure
MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPLSDGSVREALLGCASPE
CCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCEEHEEECCCCCCHHHHHHHHCCCCH
CYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQLHGGPEGFDKRRWQIVNQ
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCHHHHCCCCCCCCCCEEEEECC
NDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITYRATVDKPCPVNMTNHVYFNL
CCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCEEEEEE
DGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGTSFDFRSAKIIASEFLADDDQRK
CCCHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHH
VKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYTTAPALQFYSGNFLGGTPSRGTEPY
HCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCCCC
ADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEEYSSLTEYQFIAE
CCCCCEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7966338; 8905232; 9278503