Click here to switch to the map view.

The map label for this gene is seqA

Identifier: 157160167

GI number: 157160167

Start: 743341

End: 743886

Strand: Direct

Name: seqA

Synonym: EcHS_A0734

Alternate gene names: 157160167

Gene position: 743341-743886 (Clockwise)

Preceding gene: 157160162

Following gene: 157160168

Centisome position: 16.01

GC content: 49.82

Gene sequence:

>546_bases
ATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGA
CATTTTACGGCGTATGTTGAAATTTTCCGCCGCATCACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTGCGTCAC
CTGCTATCGTCGAAGCGAAGCCGGTCAAAACGATTAAAGACAAGGTTCGCGCAATGCGTGAACTTCTGCTTTCGGACGAA
TACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGC
CGAAGCAACGGAATCGTTGCACGGTCGTACACGCGTTTACTTTGCGGCAGATGAACAAACGCTGCTGAAAAATGGTAATC
AGACCAAGCCGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATC
GAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAA

Upstream 100 bases:

>100_bases
CGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTA
AGACACTGCACTGGATTAAG

Downstream 100 bases:

>100_bases
AACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACG
TCGCCCAACTGACGGCGCAA

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDE
YAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
EHIMQSMQFPAELIEKVCGTI

Sequences:

>Translated_181_residues
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDE
YAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
EHIMQSMQFPAELIEKVCGTI
>Mature_181_residues
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDE
YAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
EHIMQSMQFPAELIEKVCGTI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=181, Percent_Identity=100, Blast_Score=379, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SEQA_ECOL6 (P0AFY9)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_752704.2
- ProteinModelPortal:   P0AFY9
- SMR:   P0AFY9
- EnsemblBacteria:   EBESCT00000040652
- GeneID:   1036013
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c0774
- GeneTree:   EBGT00050000011480
- HOGENOM:   HBG296989
- OMA:   YRHIASK
- ProtClustDB:   PRK11187
- InterPro:   IPR005621
- InterPro:   IPR010985
- Gene3D:   G3DSA:1.20.1380.10

Pfam domain/function: PF03925 SeqA; SSF47598 Met_repress_like; SSF82808 Regulation_modulator_SeqA

EC number: NA

Molecular weight: Translated: 20316; Mature: 20316

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAK
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
PVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVY
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCGT
EECCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
I
C
>Mature Secondary Structure
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAK
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
PVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVY
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCGT
EECCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157